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Engineering of in vivo immune responses to DNA immunization via codelivery of costimulatory molecule genes

Nature Biotechnology volume 15, pages 641–646 (1997)Cite this article

Abstract

Nucleic acid immunization is a novel vaccination technique to induce antigen-specific immune responses. We have developed expression cassettes for cell surface markers CD80 and CD86, two functionally related costimulatory molecules that play an important role in the induction of T cell-mediated immune responses. Coimmunization of these expression plasmids, along with plasmid DNA encoding for HIV-1 antigens, did not result in any significant change in the humoral response; however, we observed a dramatic increase in cytotoxic T-lymphocyte (CTL) induction as well as T-helper cell proliferation after the coadministration of CD86 genes, in contrast, coimmunization with a CD80 expression cassette resulted in a minor, but positive increase in T-helper cell or CTL responses. This strategy may be of value for the generation of rationally designed vaccines and immune therapeutics.

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering,

    Jong J. Kirn

  2. Department of Pathology and Laboratory Medicine,

    Jong J. Kirn, Neil Trivedi, Darren M. Wilson, Michael A. Chattergoon, Kesan Dang, Sundarasamy Mahalingam, Michael G. Agadjanyan, Jean D. Boyer, Bin Wang & David B. Weiner

  3. Department of Pediatrics,

    Mark L. Bagarazzi

  4. Department ofOtorhinolaryngology/Head and Neck Surgery, University of Pennsylvania, Philadelphia, PA, 19104

    Yin Hu, Ken Kazahaya & Ara A. Chalian

  5. Apollon, Inc., Malvern, PA, 19355

    Richard Ciccarelli

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Correspondence to David B. Weiner.

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Kirn, J., Bagarazzi, M., Trivedi, N. et al. Engineering of in vivo immune responses to DNA immunization via codelivery of costimulatory molecule genes. Nat Biotechnol 15, 641–646 (1997). https://doi.org/10.1038/nbt0797-641

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  • DOI: https://doi.org/10.1038/nbt0797-641

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