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Molecular technologies for biodiversity evaluation: Opportunities and challenges

New technologies for detecting variation in DNA complement traditional methods in biodiversity.

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References

  1. Karp, A. et al. Molecular techniques in the assessment of botanical diversity. Ann. Bot. 78: 143–149.

    Article  CAS  Google Scholar 

  2. Avise, J.C. 1994. Molecular Matters, Natural History and Evolution. Chapman & Hall, London

  3. Vane-Wright, R.I. et al. 1991. Whattoprotect—Systematics and the agony of choice. Biol. Conserv. 55: 235.

    Article  Google Scholar 

  4. Clark, A.G. and Lanigan, C.M.S. 1993. Prospects for estimating nucteotide divergence with RAPDs. Mol. Biol. Ecol. 10: 1096–1111.

    CAS  Google Scholar 

  5. Moritz, C. 1994. Applications of mitochondrial DNA analysis in conservation: A critical review. Mol. Ecol. 3: 401–411.

    Article  CAS  Google Scholar 

  6. Milligan, B.G. et al. 1994. Conservation genetics: Beyond the maintenance of marker diversity. Mol. Ecol. 3: 423–435.

    Article  Google Scholar 

  7. Lande, R. 1988. Genetics and demography in biological conservation. Science 241: 14515–1460.

    Article  Google Scholar 

  8. Bruford, M.W. et al. 1992. Single locus and multi locus DMA fingerprinting. In Molecular Genetic Analysis of Populations: A Practical Approach. Hoelzel, A.R. (ed). IRL Press. Oxford.

    Google Scholar 

  9. Helentjaris, T. et al. 1985. Restriction fragment poly-rrophisrns as probes for plant diversity and their development as tools for applied plant breeding. Plant Mol. Biol. 5: 109–118.

    Article  CAS  Google Scholar 

  10. Jeffreys, A.J. et al. 1985. Hypervariabte ‘minisatellite’ regions in human DNA. Nature 314: 67–73.

    Article  CAS  Google Scholar 

  11. Caetano-Anolles, G. 1994. MAAP—A versatile and universal tool for genome analysis. Plant Mol. Biol. 25: 1011–1026.

    Article  CAS  Google Scholar 

  12. Vos, P. et al. 1995. AFLP: A new technique for DMA fingerprinting. Nucl. Acids Res. 23: 4407–4414.

    Article  CAS  Google Scholar 

  13. Demesure, B. et al. 1995. A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chtaroplast DNA in plants. Mol. Ecol. 4: 129–131.

    Article  CAS  Google Scholar 

  14. Hillis, D.M. and Dixon, M.T. 1991. Ribosomal DNA-Molecular evolution and phytogenetic inference. Quart Rev. Biol. 66: 411–453.

    Article  CAS  Google Scholar 

  15. Tautz, D. 1989. Hypervariability of simple sequences as a general source for polymorphic DNA. Nucl. Acids Res. 17: 6463.

    Article  CAS  Google Scholar 

  16. Morgante, M. and Olivieri, A.M. 1993. PCR-amplified microsateHitBS as markers in plant genetics. Plant J. 3: 175–182.

    Article  CAS  Google Scholar 

  17. Hillis, D.M. and Mable, B.K. 1996. Applications of molecular systematics. The state of the field and a look to the future, pp. 515–543, in Molecular Systematics, Edn 2. Hillis D.M., Moritz Q, and Mabte B.K. (eds). Sinauer Associates, Sunderiand,MA.

    Google Scholar 

  18. Weir, B.S. and Cockerham, C.C. 1984. Estimating F-statistics for the analysis of population structure. Evolution 38: 1358–1370.

    CAS  PubMed  Google Scholar 

  19. Goldstein, D.B. et al. 1995. An evaluation of genetic distances for use with microsatellite loci. Genetics. 139: 463–471.

    CAS  PubMed  PubMed Central  Google Scholar 

  20. Lynch, M. and Crease, T.J. 1990. The analysis of population survey data on DNA sequence variation. Mol. Biol. Evol. 7: 377–394.

    CAS  PubMed  Google Scholar 

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Karp, A., Edwards, K., Bruford, M. et al. Molecular technologies for biodiversity evaluation: Opportunities and challenges. Nat Biotechnol 15, 625–628 (1997). https://doi.org/10.1038/nbt0797-625

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