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Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus

Nature Biotechnology volume 16, pages 253–257 (1998)Cite this article

An Erratum to this article was published on 01 August 1998

Abstract

Expression of transgenes within a single generation by direct DNA injection into vertebrate embryos has been plagued by inefficient and nonuniform gene expression. We report a novel strategy for efficient and stable expression of transgenes driven by both ubiquitous and tissue-specific promoters by direct DNA injection into developing Xenopus laevis embryos. This strategy involves flanking expression cassettes of interest with inverted terminal repeat sequences (ITRs) from adeno-associated virus. Our results suggest that the ITR strategy may be generally applicable to other systems, such as zebra fish and embryonic stem cells, and may enable tissue-specific expression of transgenes in problematic contexts.

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  1. Department of Medicine, University of California, San Diego, La Jolla, CA, 92093-0613

    Yuchang Fu, Yibin Wang & Sylvia M. Evans

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  1. Yuchang Fu
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Correspondence to Sylvia M. Evans.

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Fu, Y., Wang, Y. & Evans, S. Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus. Nat Biotechnol 16, 253–257 (1998). https://doi.org/10.1038/nbt0398-253

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