Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Technique
  • Published:

Discrimination of Heterozygous from Wild-Type Mice Bearing a Mutation in the Macrophage–Colony-Stimulating Factor Gene Using Capillary Electrophoresis

Lab Animal volume 33, pages 33–35 (2004)Cite this article

Abstract

Discriminating between phenotypically indistinguishable heterozygous and wild-type mice presents a difficult situation for researchers attempting to find suitable breeding pairs. The authors describe a fast, reliable, nonradioactive method that combines PCR with capillary electrophoresis to determine genotypical differences among heterozygous (+/op), homozygous (op/op), and wild-type (+/+) mice.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: M-CSF sequence of a 134-bp product generated from mice genomic DNA.
Figure 2: Electropherograms from heterozygous, homozygous, and wild-type mice.

References

  1. Schonlau, F., Schlesiger, C., Ehrehen, J., Grabbe, S., Sorg, C. & Sunderkotter, C. Monocyte and macrophage functions in M-CSF–deficient op/op mice during experimental leishmaniasis. J. Leukoc. Biol. 73(5), 564–573 (2003).

    Article  Google Scholar 

  2. Doherty, T.M. et al. Rationale for the role of osteoclast-like cells in arterial calcification. FASEB J. 16(6), 577–582 (2002).

    Article  Google Scholar 

  3. Kaneko, H., Iizuka, K., Morohashi, T., Kokai, Y. & Fujimoto, J. Non-phenotypic detection of osteopetrotic (op/op) mutation by using PCR-SSCP analysis. Lab. Anim. 29(4), 442–446 (1995).

    Article  Google Scholar 

  4. Yoshida, H. et al. The murine mutation osteopetrosis is in the coding region of the macrophage colony stimulating factor gene. Nature 345(6274), 442–443 (1990).

    Article  Google Scholar 

  5. Gerstner, A., DeFord, J. & Papaconstantinou, J. Comparison of two PCR-based methods and automated DNA sequencing for Prop1 genotyping in Ames dwarf mice. Mutat. Res. 528(1–2), 37–44 (2003).

    Article  Google Scholar 

Download references

Acknowledgements

The authors thank Luisita Egger (Institute of Legal Medicine, Zentralkrankenhaus St. Jürgen Strasse, Bremen, Germany) and Stephanie Fischer (Working Group for Experimental and Clinical Arteriogenesis at the Department for Internal Medicine III, Albert-Ludwigs University of Freiburg, Germany) for their superb technical assistance. A grant from the Volkswagen Foundation supported this study.

Author information

Authors and Affiliations

  1. Techel is at the Institute of Legal Medicine, Central Bremen Clinic, 28205 Bremen, Germany.,

    Dieter Techel PhD

  2. and Jost are in the Working Group for Experimental and Clinical Arteriogenesis at the Department for Internal Medicine III, Bergmann, Buschmann, Albert-Ludwigs University of Freiburg, 79106 Freiburg, Germany.,

    Caroline Bergmann DVM, Ivo Buschmann MD & Marco M. Jost PhD

  3. Jost is also affiliated with BioVisioN AG, 30625 Hannover, Germany.,

    Marco M. Jost PhD

Authors
  1. Dieter Techel PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Caroline Bergmann DVM
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ivo Buschmann MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marco M. Jost PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dieter Techel PhD.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Techel, D., Bergmann, C., Buschmann, I. et al. Discrimination of Heterozygous from Wild-Type Mice Bearing a Mutation in the Macrophage–Colony-Stimulating Factor Gene Using Capillary Electrophoresis. Lab Anim 33, 33–35 (2004). https://doi.org/10.1038/laban0904-33

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/laban0904-33

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing