Abstract
The anchorage-independence of cells is closely related to their tumorigenicity. In the screening of inhibitors of anchorage-independent growth of tumor cells, anicemycin was isolated from the fermentation broth of an actinomycete strain TP-A0648. The producing strain was isolated from a leaf of Aucuba japonica collected in Toyama, Japan and identified as Streptomyces sp. based on the taxonomic data. The structure of anicemycin was elucidated as a new analog of spicamycin by NMR and MS analysis. Anicemycin inhibited the anchorage-independent growth of the human ovary cancer SKOV-3 cells with an IC50 of 0.015 µM about three times more potently than their anchorage-dependent growth.
Similar content being viewed by others
Article PDF
References
Uehara Y . Natural product origins of Hsp90 inhibitors. Curr Cancer Drug Targets 3: 325–330 ( 2003)
Zhan M, Zhao H, Han ZC . Signaling mechanisms of anoikis. Hitol Histopathol 19: 973–983 ( 2004)
Douma S, Van Laar T, Zevenhoven J, Meuwissen R, Van Garderen E, Peeper DS . Suppression of anoikis and induction of metastasis by the neurotrophic receptor TrkB. Nature 430: 1034–1040 ( 2004)
Igarashi Y, Iida T, Sasaki T, Saito N, Yoshida R, Furumai T . Isolation of actinomycetes from live plants and evaluation of antiphytopathogenic activity of their metabolites. Actinomycetol 16: 9–13 ( 2002)
Igarashi Y, Sekine A, Fukazawa H, Uehara Y, Yamaguchi K, Endo Y, Okuda T, Furumai T, Oki T . Anicequol, a novel inhibitor for anchorage-independent growth of tumor cells from Penicillium aurantiogriseum Dierckx TP-F0213. J Antibiot 55: 371–376 ( 2002)
Shirling EB, Gottlieb D . Methods for characterization of Streptomyces species. Intern J Syst Bact 16: 313–340 ( 1966)
Waksman SA ( Ed.). Classification, identification and description of genera and species. In The Actinomycetes, Vol. II. Williams & Wilkins, Baltimore, pp. 328–334 ( 1961)
Arai T ( Ed.). Culture Media for Actinomycetes. The Society for Actinomycetes Japan, Tokyo ( 1975)
Lechevalier HA, Lechevalier MP . A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales, Ed., Prauser H. pp. 393–405. Jena, Gustav Fischer Verlag ( 1970)
Staneck JL, Roberts GD . Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28: 226–231 ( 1974)
Furumai T, Eto K, Sasaki T, Higuchi H, Onaka H, Saito N, Fujita T, Naoki H, Igarashi Y . TPU-0037-A, B, C and D, novel lydicamycin congeners with anti-MRSA activity from Streptomyces platensis TP-A0598. J Antibiot 55: 873–880 ( 2002)
Pouchert CJ, Behnke J . The Aldrich Library of 13C and 1H FT NMR Spectra, Edition I, Volume I. Aldrich Chemical Company, inc. pp. 782–783 ( 1993)
Hayakawa Y, Nakagawa M, Kawai H, Tanabe K, Nakayama H, Shimazu A, Seto H, Otake N . Studies on the differentiation inducers of myeloid leukemic cells III. Spicamycin, a new inducer of differentiation of HL-60 human promyelocytic leukemia cells. J Antibiot 36: 934–937 ( 1983)
Acton EM, Ryan KJ, Luetzow AE . Antitumor septacidin analogues. J Med Chem 20: 1362–1370 ( 1977)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Igarashi, Y., Ootsu, K., Onaka, H. et al. Anicemycin, a New Inhibitor of Anchorage-independent Growth of Tumor Cells from Streptomyces sp. TP-A0648. J Antibiot 58, 322–326 (2005). https://doi.org/10.1038/ja.2005.40
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/ja.2005.40
Keywords
This article is cited by
-
Discovery of novel septacidin congeners from a high yield heterologous expression strain Streptomyces albus 1597
The Journal of Antibiotics (2022)
-
Biosynthesis of 4-aminoheptose 2-epimers, core structural components of the septacidins and spicamycins
The Journal of Antibiotics (2014)