Abstract
A resynthesized Brassica napus line (AACC, 2n = 38) was successively backcrossed to its parental B. campestris (AA, 2n = 20) to develop monosomic addition lines that contain the different chromosomes of the other parent B. alboglabra (CC, 2n = 18). Special emphasis was put on identifying monosomic addition lines with B. alboglabra chromosome(s) carrying genes for black seed colour. Four different types of monosomic addition plants were obtained. One of them harboured a B. alboglabra chromosome with gene(s) for black seed colour while another type of monosomic addition plant had the B. alboglabra chromosome with the gene for white flower colour. The remaining two carried hitherto unmarked C-genome chromosomes and were distinguished from each other by the morphology of the alien chromosome at diakinesis of meiosis. Meiotic studies revealed that the monosomic addition plants for the white flower colour or black seed colour had a higher trivalent frequency in the pollen mother cells when compared with the two unmarked monosomic addition plants. Two different types of double-monosomic addition plants were also obtained. Such double-monosomic addition plants will be useful to reveal intragenomic homoeology of the B. alboglabra chromosomes.
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References
Attia, T, and Röbbelen, G. 1986. Cytogenetic relationship within cultivated Brassica analysed in amphihaploids from the three diploid ancestors. Can J Genet Cytol, 28, 323–329.
Attia, T, Busso, C, and Röbbelen, G. 1987. Digenomic triploids for an assessment of chromosome relationships in the cultivated diploid Brassica species. Genome, 29, 326–330.
Banga, S S. 1988. C-genome chromosome substitution lines in Brassica juncea (L.) Coss. Genetica, 77, 81–84.
Chen, B Y, and Heneen, W K. 1992. Inheritance of seed colour in Brassica campestris L. and breeding for yellow-seeded B. napus L. Euphytica, 59, 157–163.
Chen, B Y, Heneen, W K, and Jönsson, R. 1088. Resynthesis of Brassica napus L. through interspecific hybridization between B. alboglabra Bailey and B. campestris L. with special emphasis on seed colour. Plant Breed, 101, 52–59.
Chen, B Y, Heneen, W K, and Simonsen, V. 1989. Comparative and genetic studies of isozymes in resynthesized and cultivated Brassica napus L., B. campestris L. and B. alboglabra Bailey. Theor Appl Genet, 77, 673–679.
Chen, B Y, Simonsen, V, Lannér-Herrera, C, and Heneen, W K. 1992. A Brassica campestris-alboglabra addition line and its use for gene mapping, intergenomic gene transfer and generation of trisomies. Theor Appl Genet, 84, 592–599.
Chevre, A M, This, R, Eber, F, Deschamps, M, Renard, M, Delseny, M, and Quiros, C F. 1991. Characterization of disomic addition lines of Brassica napus-Brassica nigra by isozyme, fatty acid, and RFLP markers. Theor Appl Genet, 81, 43–49.
Gill, B S, Friebe, B, and Endo, T R. 1991. Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum). Genome, 34, 830–839.
Jahier, J, Chevre, A M, Tanguy, A M, and Eber, F. 1989. Extraction of disomic addition lines of Brassica napus-B. nigra. Genome, 32, 408–413.
McGrath, J M, and Ouiros, C F. 1990. Generation of alien chromosome addition lines from synthetic Brassica napus: morphology, cytology, fertility, and chromosome transmission. Genome, 33, 374–383.
Naranjo, T, Roca, A, Goicoechea, P G, and Giraldez, R. 1988. Chromosome structure of common wheat: Genome reassignment of chromosomes 4A and 4B. In: T. E. Miller and R. M. D. Koebner (eds) Seventh International Wheat Genetics Symposium, vol. 1, pp. 115–120. Institute of Plant Science Research, Cambridge Laboratory, Cambridge, U.K.
Quiros, C F, Ochoa, O, Kianian, S F, and Douches, D. 1987. Analysis of the Brassica oleracea genome by the generation of B. campestris-oleracea chromosome addition lines: characterization by isozymes and rDNA genes. Theor Appl Genet, 74, 758–766.
Song, K M, Osborn, T C, and Williams, P H. 1988. Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs). I. Genome evolution of diploid and amphidiploid species. Theor Appl Genet, 75, 784–794.
Struss, D, Bellin, U, and Röbbelen, G. 1991. Development of B-genome chromosome addition lines of B. napus using different interspecific Brassica hybrids. Plant Breed, 106, 209–214.
Tai, W, and Ikonen, H. 1988. Incomplete bivalent pairing in dihaploids of Brassica napus L. Genome, 30, 450–457.
This, P, Ochoa, O, and Quiros, C F. 1990. Dissection of the Brassica nigra genome by monosomic addition lines. Plant Breed, 105, 211–220.
U, N. 1935. Genome-analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jap J Bot, 7, 389–452.
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Cheng, B., Chen, B. & Heneen, W. Addition of Brassica alboglabra Bailey chromosomes to B. campestris L. with special emphasis on seed colour. Heredity 73, 185–189 (1994). https://doi.org/10.1038/hdy.1994.118
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DOI: https://doi.org/10.1038/hdy.1994.118
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