Abstract
Two hundred and twenty individuals of the moss, Climacium americanum, from three localities in the Piedmont of North Carolina were sampled for a study of electrophoretically detectable genetic variation. Eight enzymes (ACP, ADH, ESTfl, GOT, PER, PGI, PGM, and SOD) were assayed and only two (GOT, SOD) were monomorphic at all three localities, in spite of the extreme rarity of sexual reproduction in this species. Although the var. kindbergii has generally been considered a form of C. americanum induced by submersion in water, there was a strong correlation between enzyme phenotypes and morphological characters distinguishing the two varieties of C. americanum. These data suggest that there is linkage disequilibrium among the loci coding for ACP, PGM, and PGI, and between these loci and those controlling several morphological characters that distinguish the two varieties of C. americanum.
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References
Bedford, T H B. 1938. Sex distribution in colonies of Climacium dendroides W. & M. and its relation to fruit bearing. NW Nat, 13, 213–221.
Bishop, Y M M, Feinberg, S E, and Holland, P W. 1975. Discrete multivariate analysis: theory and practice, MIT Press, Cambridge, Mass.
Cardot, J, and Theriot, I. 1904. New or unrecorded mosses of North America, II. Bot Gaz, 37, 363–382.
Cardot, J, and Theriot, I. 1906. New or unrecorded mosses of North America. Bryologist, 9, 6–10.
Cardy, B J, Stuber, C W, and Goodman, M M. 1983. Techniques for starch gel electrophoresis of enzymes from maize (Zea mays L.). Institute of Statistics Mimeo Series No. 1317. North Carolina State University, Raleigh, NC. 35 pp.
Christensen, B, Berg, M, and Jelnes, J. 1982. Genotypic diversity in Oenothera laciniata (Onagraceae), a permanent translocation heterozygote. Evolution, 36, 63–69.
Crum, H A, and Anderson, L E. 1981. Mosses of Eastern North America, Columbia Univ. Press, New York.
Crum, H A W C, Steere, W C, and Anderson, L E. 1973. A new list of mosses of North America north of Mexico. Bryologist, 76, 85–130.
Cummins, H, and Wyatt, R. 1981. Genetic variability in natural populations of the moss Atrichum angustatum. Bryologist, 84, 30–38.
Daniels, R E. 1982. Isozyme variation in British populations of Sphagnum pulchrum (Braithw.) Warnst. J Bryol, 12, 1–11.
Devries, A, Van Zanten, B O, and Van Dijk, H. 1983. Genetic variability within and between populations of two species of Racopilum (Racopilaceae, Bryopsida). Lindbergia, 9, 73–80.
Ellstrand, N C, and Levin, D A. 1982. Genotypic diversity in Oenothera laciniata (Onagraceae), a permanent translocation heterozygote. Evolution, 36, 63–69.
Grout, A J. 1901. Climacium Web. & Mohr., Iter. Suec. 96. 1804. Bryologist, 4, 54.
Horton, D G, and Vitt, D H. 1976. Morphological characters, relative to distribution, and taxonomic considerations of the genus Climacium. Can J Bot, 54, 1872–1883.
Jeffries, R J, and Gottlieb, L D. 1983. Genetic variation within and between populations of the asexual plant Puccinellia × phyganodes. Can J Bot, 61, 774–779.
Kelley, W A, and Adams, R P. 1977. Preparation of extracts from Juniper leaves for electrophoresis. Phytochemistry, 16, 513–516.
Krzakowa, M. 1977. Isoenzymes as markers of inter- and intraspecific differentiation in hepatics. Bryophyt Biblio, 13, 427–434.
Krzakowa, M, and Szweykowski, J. 1979. Isoenzyme polymorphism in natural populations of a liverwort Plagiochila asplenioides. Genetics, 93, 711–719.
Lewontin, R C. 1974. The Genetic Basis of Evolutionary Change, Columbia University Press, New York.
Mitton, J B, and Koehn, R K. 1973. Population genetics of marine Pelecypods. III. Epistasis between functionally related isozymes of Mytilus edulis. Genetics, 73, 489–496.
Renauld, F, and Cardot, J. 1890. New mosses of North America. IV. Bot Gaz, 15, 57–62.
Roughgarden, J. 1979. Theory of population genetics and ecology: an introduction, MacMillan Publishing Co., Inc.; New York.
Shaw, J. 1987. Growth form variation within and among populations of Climacium americanum Brid. Acta Bot Hung (in press).
Shaw, D R, and Prasad, R. 1970. Starch gel electrophoresis of enzymes—a compilation of recipes. Biochem Gen, 4, 297–320.
Soltis, D E, Haufler, C H, Darrow, D C, and Gastony, G J. 1983. Starch gel electrophoresis of ferns: a compilation of grinding buffers, gel and electrode buffers, and staining schedules. Am Fern J, 73, 9–27.
Szweykowski, J, and Krzakowa, M. 1979. Variation of four enzyme systems in Polish populations of Conocephalum conicum (L.) Dum. (Hepaticae, Marchantiales). Bull Acad Polon Sci, Ser Sci Biol, 27, 37–41.
Usberti, J A, and Jain, S K. 1978. Variation in Panicum maximum: a comparison of sexual and asexual populations. Botanical Gazette, 139, 112–116.
Zielinski, R. 1984a. Genetic variation and evolution of the liverwort genus Pellia with special reference to Central European territory. I. Electrophoretic evidence of cross-fertilization in the monoecious Pellia epiphilla, n = 9. J Hattori Bot Lab, 56, 255–262.
Zielinski, R. 1984b. Genetic variation and evolution of the liverwort genus Pellia with special reference to Central European territory. II. Electrophoretic and cytological study of the Pellia epiphylla and P. borealis complex. J Hattori Bot Lab, 56, 263–269.
Zielinski, R. 1986. Cross-fertilization in the monoecius Pellia borealis, n = 18, and spatial distribution of two peroxidase genotypes. Heredity 56, 299–304.
Zielinski, R, Szweykowski, J, and Rutkowska, E. 1985. A further electrophoretic study of peroxidase isoenzyme variation in Pellia epiphylla (L.) Dum. from Poland, with special reference to the status of Pellia borealis Lorbeer. Monogr Syst Bot Missouri Bot Gard, 11, 199–209.
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Shaw, J., Meagher, T. & Harley, P. Electrophoretic evidence of reproductive isolation between two varieties of the moss, Climacium americanum. Heredity 59, 337–343 (1987). https://doi.org/10.1038/hdy.1987.140
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DOI: https://doi.org/10.1038/hdy.1987.140
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