Abstract
C-banding of P. pedestris chromosomes demonstrated the presence of small procentromeric heterochromatic blocks on all members of the complement. The telocentric X-chromosomes and all of the medium sized autosomes also had small distally located C-bands and the megarmeric M7 chromosome consistently had a number of interstitial bands. The metacentric neo X chromosome found in some populations has a distinctive C-banding pattern in that although the distal C-block of the free X is retained, the centromeric heterochromatin is quite asymetrical with most of the procentric C-band of the X apparently having been lost during or subsequent to the X-autosome fusion event. Two banding polymorphisms were noted, an extra C-band on the S10 chromosome and variability in size of the distal C-band on the M6. B-chromosomes did not appear to have any C-band material other than a small procentric block.
G-banding gave no additional information as the chromosomes appeared light staining only in those regions which were C-positive. Use of the fluorochromes Hoechst 33258 and Chromomycin A3 showed that the heterochomatin of P. pedestris is relatively rich in G + C bases.
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Barton, N, and Hewitt, G M. 1981a. A chromosomal cline in the grasshopper Podisma pedestris. Evolution, 35, 1008–1018.
Barton, N, and Hewitt, G M. 1981b. The genetic basis of hybrid variability in the grasshopper Podisma pedestris. Heredity, 47, 367–383.
Cardosa, H, and Dutra, A. 1979. The neo X neo Y sex pair in Acrididae; its structure and association. Chromosoma (Berl.), 70, 323–336.
Comings, D E. 1975. Mechanisms of chromosome banding. VIII. Hoechst 33258-DNA interactions. Chromosoma (Berl.), 52, 229–243.
Das, B C, Raman, R, and Sharma, T. 1979. Chromosome condensation and Hoechst 33258 fluorescence in meiotic chromosomes of the grasshopper Spathosternum prasiniferum (Walker). Chromosoma (Berl.), 70, 251–258.
Dev, V G, Miller, D A, Tantrahavi, R, Schreck, R R, Roderick, T H, Erlanger, B F, and Miller, O J. 1975. Chromosome markers in Mus musculus: differences in C-banding between the subspecies M.m.musculus and M.m.molossinus. Chromosoma (Berl.), 53, 335–44.
Halliday, B, Barton, N, and Hewitt, G M. 1983. Electrophoretic analysis of a chromosomal hybrid zone in the grasshopper Podisma pedestris. Biol J Linn Soc, 19, 51–62.
Hewitt, G M. 1975. A sex chromosome hybrid zone in the grasshopper Podisma pedestris (Orthoptera: Acrididae). Heredity, 35, 375–87.
Hewitt, G M, and Barton, N. 1981. The structure and maintenance of hybrid zones as exemplified by Podisma pedestris. In R. L. Blackman, Hewitt, G. M. and Ashburner, M. (eds) Blackwell Scientific Publishers.
Hewitt, G M, and John, B. 1972. Interpopulation sex-chromosome polymorphism in the grasshopper Podisma pedestris. II Population parameters. Chromosoma (Berl.) 37, 23–42.
Hilwig, I, and Gropp, A. 1972. Staining of constitutive heterochromatin mammalian chromosomes with a new fluorochrome. Exp Cell Res, 75, 122–126.
John, B. 1981. Heterochromatin variation in natural populations. In Chromosomes Today, 7, 128–137.
John, B, and Hewitt, G M. 1970. Interpopulation sex chromosome polymorphism in the grasshopper Podisma pedestris. I. Fundamental facts. Chromosoma (Berl.), 31, 291–308.
John, B, and King, M. 1977. Heterochromatin variation in Cryptobothrus chrysophorus II. Patterns of C-banding. Chromosoma (Berl.), 65, 59–79.
John, B, and Miklos, G L G. 1979. Functional aspects of satellite DNA and heterochromatin. Int Rev Cytol, 58, 1–114.
Jorgenson, K F, Van De Sande, J H, and Lin, C C. 1978. The use of base pair specific DNA banding agents as affinity labels for the study of mammalian chromosomes. Chromosoma (Berl.), 68, 287–302.
Martin, R F, and Holmes, H. 1983. Use of an I125-labelled DNA to probe DNA structure. Nature (Lond.), 302, 452–454.
Schweizer, D. 1976. Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma (Berl.), 58, 307–324.
Schweizer, D. 1981. Counterstain-enhanced chromosome banding. Hum Genet, 57, 1–14.
Webb, G C. 1976. Chromosome organization in the Australian plague locust, Chortoicetes terminifera I. Banding relationships of the normal and supernumerary chromosomes. Chromosoma (Berl.) 55, 229–49.
Webb, G C, and Westerman, M. 1978. G- and C-banding in the Australian grasshopper Phaulacridium vittatum. Heredity, 41, 131–36.
Weisblum, B, and Haenssler, E. 1974. Fluometric properties of the bisbenzimidazole derivative Hoechst 33258, a fluorescent probe specific for At concentration inc chromosomal DNA. Chromosoma (Berl.) 46, 255–260.
Zhan, T S, Pathak, S, and Liang, J. 1984. Induction of G-bands in the chromosomes of Melanoplus sanguinipes (Orthoptera, Acrididae). Can J Genet Cytol 26, 354–359.
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Westerman, M., Hewittt, G. Chromosome banding in Podisma pedestris. Heredity 55, 157–161 (1985). https://doi.org/10.1038/hdy.1985.85
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DOI: https://doi.org/10.1038/hdy.1985.85
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