Abstract
An oestrogen sensitive rat mammary tumour was grown in two groups of female and one group of male hooded rats. The male group and one of the female groups were supplemented with oestrogen. The tumours grew most rapidly in the female supplemented group. When the tumours reached 1.5 cm in diameter they were harvested and the cell cycle distribution and number of cells actively synthesising DNA (bromodeoxyuridine (BrdU) labelling index) determined in each case. Chemical extracts were prepared from each tumour and the concentration of phosphorus-containing metabolites determined using high resolution NMR spectroscopy. The concentration of phosphocholine was found to correlate strongly with the number of cells in S-phase and the number of cells labelled with BrdU, whilst a highly significant negative correlation was observed between these two parameters and glycerophosphocholine. The concentration of phosphoethanolamine did not correlate with either of these measures of proliferation rate. The concentration of glycerophosphorylethanolamine showed a weak negative correlation with the number of cells in S-phase.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 24 print issues and online access
$259.00 per year
only $10.79 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Smith, T., Eccles, S., Ormerod, M. et al. The phosphocholine and glycerophosphocholine content of an oestrogen-sensitive rat mammary tumour correlates strongly with growth rate. Br J Cancer 64, 821–826 (1991). https://doi.org/10.1038/bjc.1991.407
Issue Date:
DOI: https://doi.org/10.1038/bjc.1991.407
This article is cited by
-
Probing the PI3K/Akt/mTor pathway using 31P-NMR spectroscopy: routes to glycogen synthase kinase 3
Scientific Reports (2016)
-
[methyl-3H]Choline incorporation into MCF7 tumour cells: correlation with proliferation
European Journal of Nuclear Medicine and Molecular Imaging (2005)
-
Noninvasive estimation of tumour viability in a xenograft model of human neuroblastoma with proton magnetic resonance spectroscopy (1H MRS)
British Journal of Cancer (2003)
-
Use of radiolabelled choline as a pharmacodynamic marker for the signal transduction inhibitor geldanamycin
British Journal of Cancer (2002)
-
Exposure of Human Breast Cancer Cells to the Anti-inflammatory Agent Indomethacin Alters Choline Phospholipid Metabolites and Nm23 Expression
Neoplasia (2002)