Abstract
To assist in the description of the cellular heterogeneity present in normal and neoplastic urothelium, a panel of monoclonal antibodies (MoAbs) was raised against human transitional cell carcinoma (TCC) of the urinary bladder. All immunizations were carried out using whole cells and membrane preparations from well differentiated human TCCs. Two fusions produced 145 hybridomas. Following primary screening by ELISA and secondary screening with immunohistochemistry, three useful antibodies were identified. MoAb 35.48 binds to all cell layers of the normal urothelium and well differentiated tumours, but not to the majority of poorly differentiated tumours. MoAb 21.48 binds preferentially to the basal cell layer of normal urothelium and to some well differentiated papillary TCCs, but poorly differentiated tumours exhibit diffusely positive staining. MoAb 21.48 also shows cross-reactivity with basal cell layers of other epithelia. MoAb 5.48 binds preferentially to the superficial cell layers of normal urothelium and well differentiated TCCs, but exhibits less binding in poorly differentiated tumours with loss of the preferential superficial staining. Quantitative flow cytometric studies indicate that MoAb 5.48 binds to a cell-surface antigen which is present on significantly fewer cells of poorly differentiated tumours than on either normal urothelium (P less than 0.05), or well differentiated tumours (P = 0.05).
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 24 print issues and online access
$259.00 per year
only $10.79 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dotsikas, G., Konowalchuk, T., Major, P. et al. Cellular heterogeneity in normal and neoplastic human urothelium: A study using murine monoclonal antibodies. Br J Cancer 56, 439–444 (1987). https://doi.org/10.1038/bjc.1987.220
Issue Date:
DOI: https://doi.org/10.1038/bjc.1987.220
This article is cited by
-
Normal and neoplastic urothelial stem cells: getting to the root of the problem
Nature Reviews Urology (2012)
-
Preclinical studies of monoclonal antibodies for intravesical radioimmunotherapy of human bladder cancer
Cell Biophysics (1994)
-
Monoclonal antibodies for intravesical radioimmunotherapy of human bladder cancer
Cell Biophysics (1993)
-
Cellular heterogeneity in human transitional cell carcinoma: an anlysis of optical properties and lectin binding
The Histochemical Journal (1992)