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Loss of cyclin D1 in concert with deregulated estrogen receptor α expression induces DNA damage response activation and interrupts mammary gland morphogenesis

Oncogene volume 27, pages 3186–3193 (2008)Cite this article

Abstract

We have previously shown that increased and deregulated estrogen receptor α expression in the mammary gland leads to the development of proliferative disease and cancer. To address the importance of cyclin D1 in ERα-mediated mammary tumorigenesis, we crossed ERα-overexpressing mice with cyclin D1 knockout mice. Mammary gland morphogenesis was completely interrupted in the ERα-overexpressing cyclin D1-deficient triple transgenic mice. In addition to a highly significant reduction in mammary epithelial cell proliferation, cyclin E was upregulated resulting in DNA damage checkpoint activation and apoptosis. This imbalance between proliferative and apoptotic rates in conjunction with remarkable structural defects and cellular disorganization in the terminal end buds interrupted ductal morphogenesis. Interestingly, the structure of the mammary fat pad was fundamentally altered by the consequences of overexpressing ERα in the epithelial cells in the absence of cyclin D1 illustrating how alterations in the epithelial compartment can impact surrounding stromal composition. Transplantation of embryonic ERα-overexpressing and cyclin D1-deficient mammary epithelium into the cleared fat pad of wild-type mice did not rescue the aberrant mammary gland phenotype indicating that it was intrinsic to the mammary epithelial cells. In conclusion, although cyclin D1 is not essential for proliferation of normal mammary epithelial cells, ERα-overexpressing cells are absolutely dependent on cyclin D1 for proliferation. This differential requirement for cyclin D1 in normal vs abnormal mammary epithelial cells supports the application of cyclin D1 inhibitors as therapeutic interventions in ERα-overexpressing breast cancers.

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Acknowledgements

This work was supported by grant NCI, NIH 1RO1CA112176 (PAF) and DOD Breast Cancer Research Predoctoral Award W81XWH-05-1-0302 (MSF).

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Authors and Affiliations

  1. Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA

    M S Frech, K M Torre & P A Furth

  2. Laboratory of Genetics and Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA

    G W Robinson

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  1. M S Frech
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  2. K M Torre
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  3. G W Robinson
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  4. P A Furth
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Correspondence to M S Frech.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc).

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Frech, M., Torre, K., Robinson, G. et al. Loss of cyclin D1 in concert with deregulated estrogen receptor α expression induces DNA damage response activation and interrupts mammary gland morphogenesis. Oncogene 27, 3186–3193 (2008). https://doi.org/10.1038/sj.onc.1210974

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