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The 3p21 candidate tumor suppressor gene BAF180 is normally expressed in human lung cancer

Oncogene volume 24, pages 2735–2738 (2005)Cite this article

Abstract

BAF180 encoding a subunit of the human SWI/SNF chromatin remodeling complex maps to 3p21, in a region where frequent allele loss has been detected in lung cancer. BAF180 can be mutated and has tumor suppressing properties in breast cancer. In addition, another member of this complex, hSNF5/INI1, is a known tumor suppressor gene (TSG) for malignant rhabdoid and childhood central nervous system tumors. Thus, BAF180 is a strong candidate TSG for lung cancer. The objective of this study was to determine whether BAF180 mRNA or protein expression was inactivated or abnormal in lung cancers to prompt detailed DNA promoter methylation or mutational analyses. In 30 non-small-cell and 26 small-cell lung cancer cell lines, most of which had 3p21 allele loss, BAF180 mRNA and protein expression were evaluated by RT–PCR using three sets of primers and Western blotting using two anti-BAF180 antibodies. In all cases, BAF180 was expressed and no abnormal size BAF180 protein was detected. Finally, we found no amino-acid sequence coding mutations in five non-small-cell and five small-cell lung cancer cell lines, while we did find a new splicing isoform of BAF180 (AY281068). We conclude that abnormalities of BAF180 are not frequently involved in the pathogenesis of lung cancer.

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Acknowledgements

This work was supported by NC1 Grants P50 CA70907 (Lung Cancer SPORE), CA71618, and the Gillson Longenbaugh Foundation.

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Authors and Affiliations

  1. Hamon Center for Therapeutic Oncology Research, The University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd, Dallas, TX, 75390-8593, USA

    Ikuo Sekine, Mitsuo Sato, Noriaki Sunaga, Shinichi Toyooka, Michael Peyton, Adi F Gazdar & John D Minna

  2. Institute for Cancer Genetics, Columbia University, 1150 St Nicholas Avenue, New York, NY, 10032, USA

    Ramon Parsons

  3. Laboratory of Genetics, National Institute on Aging, National Institute of Health, 333 Cassell Drive, TRIAD Center Room 4000, Baltimore, MD, 21224, USA

    Weidong Wang

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  1. Ikuo Sekine
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  2. Mitsuo Sato
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  3. Noriaki Sunaga
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  4. Shinichi Toyooka
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  5. Michael Peyton
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  8. Adi F Gazdar
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Correspondence to John D Minna.

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Sekine, I., Sato, M., Sunaga, N. et al. The 3p21 candidate tumor suppressor gene BAF180 is normally expressed in human lung cancer. Oncogene 24, 2735–2738 (2005). https://doi.org/10.1038/sj.onc.1207694

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