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Comparative biology of rAAV transduction in ferret, pig and human airway epithelia

Gene Therapy volume 14, pages 1543–1548 (2007)Cite this article

Abstract

Differences between rodent and human airway cell biology have made it difficult to translate recombinant adeno-associated virus (rAAV)-mediated gene therapies to the lung for cystic fibrosis (CF). As new ferret and pig models for CF become available, knowledge about host cell/vector interactions in these species will become increasingly important for testing potential gene therapies. To this end, we have compared the transduction biology of three rAAV serotypes (AAV1, 2 and 5) in human, ferret, pig and mouse-polarized airway epithelia. Our results indicate that apical transduction of ferret and pig airway epithelia with these rAAV serotypes closely mirrors that observed in human epithelia (rAAV1>rAAV2≅rAAV5), while transduction of mouse epithelia was significantly different (rAAV1>rAAV5≫rAAV2). Similarly, ferret, pig and human epithelia also shared serotype-specific differences in the polarity (apical vs basolateral) and proteasome dependence of rAAV transduction. Despite these parallels, N-linked sialic acid receptors were required for rAAV1 and rAAV5 transduction of human and mouse airway epithelia, but not ferret or pig airway epithelia. Hence, although the airway tropisms of rAAV serotypes 1, 2 and 5 are conserved better among ferret, pig and human as compared to mouse, viral receptors/co-receptors appear to maintain considerable species diversity.

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Acknowledgements

This work was supported by NIH RO1 HL58340 (JFE), the Center for Gene Therapy (DK54759) and Targeted Genetics Corporation. We also gratefully acknowledge the Roy J Carver Chair in Molecular Medicine (JFE) and Dr Christine Blaumueller for editorial assistance.

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Authors and Affiliations

  1. Department of Anatomy & Cell Biology, College of Medicine, The University of Iowa, Iowa City, IA, USA

    X Liu, M Luo, C Guo, Z Yan & J F Engelhardt

  2. College of Life Science, Ningxia University, Yingchuan, Ningxia, China

    C Guo, Y Wang & J F Engelhardt

  3. Department of Internal Medicine, College of Medicine, The University of Iowa, Iowa City, IA, USA

    J F Engelhardt

  4. Center for Gene Therapy of Cystic Fibrosis and Other Genetic Diseases, College of Medicine, The University of Iowa, Iowa City, IA, USA

    X Liu, Z Yan & J F Engelhardt

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  1. X Liu
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Correspondence to J F Engelhardt.

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Liu, X., Luo, M., Guo, C. et al. Comparative biology of rAAV transduction in ferret, pig and human airway epithelia. Gene Ther 14, 1543–1548 (2007). https://doi.org/10.1038/sj.gt.3303014

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