Genetic variations in ARE1 mediate grain yield by modulating nitrogen utilization in rice

Wang, Qing; Nian, Jinqiang; Xie, Xianzhi; Yu, Hong; Zhang, Jian; Bai, Jiaoteng; Dong, Guojun; Hu, Jiang; Bai, Bo; Chen, Lichao; Xie, Qingjun; Feng, Jian; Yang, Xiaolu; Peng, Juli; Chen, Fan; Qian, Qian; Li, Jiayang; Zuo, Jianru

doi:10.1038/s41467-017-02781-w

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Published: 21 February 2018

Genetic variations in ARE1 mediate grain yield by modulating nitrogen utilization in rice

Qing Wang ORCID: orcid.org/0000-0003-2110-926X^1,2^na1,
Jinqiang Nian ORCID: orcid.org/0000-0002-5689-0335¹^na1,
Xianzhi Xie³^na1,
Hong Yu ORCID: orcid.org/0000-0002-1748-8693¹,
Jian Zhang¹,
Jiaoteng Bai^1,2,
Guojun Dong⁴,
Jiang Hu⁴,
Bo Bai³,
Lichao Chen^1,2,
Qingjun Xie ORCID: orcid.org/0000-0002-6372-3260^1,2,
Jian Feng^1,2,
Xiaolu Yang^1,2,
Juli Peng¹,
Fan Chen^2,5,
Qian Qian⁴,
Jiayang Li ORCID: orcid.org/0000-0002-0487-6574^1,2 &
…
Jianru Zuo ORCID: orcid.org/0000-0002-9605-3822^1,2,6

Nature Communications volume 9, Article number: 735 (2018) Cite this article

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Abstract

In crops, nitrogen directly determines productivity and biomass. However, the improvement of nitrogen utilization efficiency (NUE) is still a major challenge in modern agriculture. Here, we report the characterization of are1, a genetic suppressor of a rice fd-gogat mutant defective in nitrogen assimilation. ARE1 is a highly conserved gene, encoding a chloroplast-localized protein. Loss-of-function mutations in ARE1 cause delayed senescence and result in 10–20% grain yield increases, hence enhance NUE under nitrogen-limiting conditions. Analysis of a panel of 2155 rice varieties reveals that 18% indica and 48% aus accessions carry small insertions in the ARE1 promoter, which result in a reduction in ARE1 expression and an increase in grain yield under nitrogen-limiting conditions. We propose that ARE1 is a key mediator of NUE and represents a promising target for breeding high-yield cultivars under nitrogen-limiting condition.

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Introduction

Nitrogen is an essential element for all living organisms and the nitrogen nutrients derived from plants are primary sources for humans and animals. In crops, nitrogen is one of the most predominantly limiting factors for productivity and breeding cultivars with the improved nitrogen utilization efficiency (NUE) is urgently demanding for sustainable development of agriculture^1,2. Most non-legume plants absorb inorganic nitrogen compounds from soil, followed by converting inorganic into organic nitrogen compounds, a process known as nitrogen assimilation^2,3,4,5. Plants mainly acquire nitrate (NO₃⁻) and ammonium (NH₄⁺) from soil by the plasma membrane-localized transporters. After entering the plant cell, nitrate is sequentially reduced to nitrite and ammonium by nitrate reductase and nitrite reductase, respectively, of which ammonium is converted into organic nitrogen compounds by the primary assimilation^2,4,6.

The biochemical framework of nitrogen assimilation has been well established, while the underpinning regulatory mechanisms are not well understood^1,2,5. The primary nitrogen assimilation is mediated by the coupled reactions catalyzed by glutamine synthetase (GS) and glutamate synthase, the latter one also known as glutamine:2-oxoglutarate amidotransferase (GOGAT). GS catalyzes the conversion of glutamate into glutamine by incorporating a molecule of ammonia, whereas GOGAT transfers an amide group from glutamine to 2-oxoglutarate to produce two molecules of glutamate. Two types of GOGAT, Fd-GOGAT and NADH-GOGAT, have been characterized, which use different reductants with distinctive tissue specificities and biochemical properties^7,8,9. The GS/GOGAT cycle is highly conserved in the plant kingdom, ranging from algae to higher plants. In rice (Oryza sativa L.), weak mutant alleles of fd-gogat (also known as abnormal cytokinin response1 or abc1) causes severe developmental defects and enhances bacterial blight resistance, whereas a null mutant allele is seedling lethal^10,11,12. In this study, we functionally characterize a genetic suppressor of abc1-1, designated as are1 (for abc1-1 repressor1), which partially rescues the nitrogen assimilation-deficiency phenotype of abc1-1. Null mutations in ARE1 cause the delayed senescence, the enhanced NUE, and the increased grain yield under nitrogen-limiting conditions. We also find that the natural variations in the ARE1 promoter are directly associated with its expression and grain yield, thereby identifying ARE1 as a key mediator of NUE and a promising locus for the genetic improvement of NUE in rice.

Results

Characterization of the are1 mutant

We previously identified a rice abc1 mutant (in the Nipponbare or NPB background) that shows a typical nitrogen deficient syndrome, including the reduction in the plant height, tiller number, chlorophyll level, and grain yield¹⁰. ABC1 encodes Fd-GOGAT, a key enzyme in the GS/GOGAT cycle for nitrogen assimilation¹⁰. We subsequently performed a genetic screen for abc1-1 repressor (are) mutants by examining the phenotype of the plant height, tiller number, and leaf color under field growth conditions. Two of those identified mutations, are1-1 and are1-2, partially rescued the pleiotropic phenotype of abc1-1, including defects in the plant height, tiller number and the leaf chlorophyll content (Fig. 1a–d and Supplementary Fig. 1a). In an allelism test, all F₁ progeny obtained from a cross between are1-1 and are1-2 showed an abc1-supressor phenotype (Fig. 1a and Supplementary Fig. 1a), demonstrating that these two mutations are allelic. The abc1-1 are1-1 and abc1-1 are1-2 double mutants were backcrossed with wild-type NPB plants three times, respectively, and the are1-1 and are1-2 single mutants were identified from respective BC₂F₂ progenies. These two alleles were phenotypically indistinguishable and are1-1 was used in most experiments hereafter unless indicated otherwise.

During the early vegetative growth stages, are1 showed a phenotype similar to wild type. At the later growth stages, are1 had a slightly increased plant height and reduced tiller number (Fig. 1e). The heading date of are1-1 was often delayed for 3–5 days than wild type. At the grain-filling stage, are1-1 plants exhibited a stay-green phenotype (Fig. 1e, f). Consistent with this result, the accumulation of chlorophylls in are1-1 was significantly higher than that in wild type after the heading stage (Fig. 1g), indicating that are1-1 causes delayed senescence and prolonged efficient photosynthetic activities.

The abc1-1 is a leaky mutant allele, retaining approximately 50–60% Fd-GOGAT activity, and the T-DNA insertional mutant abc1-2 is a null allele¹⁰. Whereas the are1-1 mutation did not cause detectable effects on the accumulation of Fd-GOGAT protein (Supplementary Fig. 1b), the Fd-GOGAT enzymatic activity in abc1-1 are1-1 was nearly restored to that in the wild type (Fig. 1h). However, the abc1-2 are1-1 double mutant showed an abc1-2-like or seedling-lethal phenotype (Supplementary Fig. 1c), indicating that are1-1 is incapable of rescuing the phenotype of a null mutation in ABC1/Fd-GOGAT. These results suggest that the suppressing effect of are1 on abc1-1 is partly attributable to the rescued activity of Fd-GOGAT.

Enhanced tolerance of are1 to nitrogen deficiency

Given that the are1 mutations largely rescue the nitrogen deficient phenotype of abc1-1, ARE1 may be involved in nitrogen metabolism. Plants evolved various strategies for survival when grown under nutrient limiting environments, including the increase of the root-to-shoot ratio¹³. Compared to the wild type, are1-1 showed an increased root-to-shoot ratio under nitrogen deficiency condition, mainly resulted from increased root biomass (Fig. 2a and Supplementary Fig. 2a, b). Consistently, are1-1 seedlings retained a higher level of chlorophylls than the wild type (Fig. 2b and Supplementary Fig. 2c). Nitrogen depletion or reduction rapidly induced the expression of key genes involved in ammonia transport and nitrogen assimilation in wild-type rice seedlings, and this induction was reduced in are1-1 (Fig. 2c, d and Supplementary Fig. 2d–k), indicating that are1-1 is less sensitive to nitrogen starvation.

The reduced sensitivity of are1 to nitrogen starvation may be contributed by the increased accumulation of nitrogen in planta when grown under sufficient nitrogen condition. To test this possibility, we analyzed the nitrogen content in the field-grown plants. The total nitrogen contents in various organs were significantly higher in are1-1 than that in wild type plants, and the nitrogen uptake efficiency (NUpE) and NUE were consequently increased when the nitrogen fertilizer was progressively reduced (Fig. 2e, f and Supplementary Table 1). Consequently, the expression of the ammonia transport and nitrogen assimilation genes was increased in are1-1 than that in wild type plants under limited nitrogen supplies (Fig. 2g, h and Supplementary Fig. 2l–n), suggesting that ARE1 is negatively correlated to NUpE and NUE.

Molecular characterization of ARE1

In a genetic analysis, all F₁ progeny (18 plants) obtained from a cross between are1-1 and NPB showed a wild-type-like phenotype by analyzing the senescence phenotype. In the F₂ population, the normal and delayed senescence phenotypes were segregated approximately in a ratio of 3:1 (561:196 = 2.86:1; χ²_C < χ²_0.05 = 3.84), indicating that are1 is a recessive Mendelian mutation in a single nuclear gene. Using a map-based cloning approach, we genetically mapped ARE1 to chromosome 8 in a region containing 13 predicted open reading frames (ORFs) (Fig. 3a; see Methods for details). DNA sequencing analysis revealed the presence of a single base deletion (A-899; the putative transcription start is referred to as +1) in the fourth exon of LOC_Os08g12780 in the are1-1 genome, resulting in frame-shift mutations after this base (Fig. 3a and Supplementary Fig. 3). In the are1-2 genome, a single base substitution (C-T) in the sixth exon caused a highly conserved Pro residue being substituted with Leu (Fig. 3a and Supplementary Fig. 3).

To verify the identity of the ARE1 candidate gene, we performed a molecular complementation test by transforming of abc1-1 are1-1 plants with a wild-type genomic fragment containing the entire LOC_Os08g12780 gene under the control of its native promoter (~2.3 kb) and 3′-untranscribed region (~1.9 kb). All the T₁ transgenic plants showed an abc1-1-like phenotype (Fig. 3b), indicating that the transgene reverts the suppressing effect of are1-1 on the abc1-1 mutation. When transformed into are1-1 plants, the transgene rescued the delayed senescence phenotype of the mutant (Fig. 3c–e). These results demonstrate that LOC_Os08g12780 represents ARE1.

ARE1 encodes a putative protein that is highly conserved from lower to higher plants with unknown function (Supplementary Figs. 3 and 4a). Overall, ARE1 shares approximately 27–85% identity with ARE1-like proteins from cyanobacteria to flowering plants (Supplementary Fig. 4a). In the rice genome, a putative protein encoded by LOC_Os02g24598 shares low homology with ARE1 (22.6% identity). ARE1 is predominately expressed in photosynthetic tissues, including leaves and leaf sheaths, with a lower expression level in culms, young panicles and spikes (Fig. 3f and Supplementary Fig. 4b–d). ARE1 was predicted as a chloroplast-localized protein containing a putative transit signal peptide of 47 amino acid residues (Supplementary Figs. 3 and 4e, f). When transiently expressed in rice protoplasts, ARE1-YFP (yellow fluorescence protein) fusion protein was localized in chloroplasts, whereas the deletion of the putative transit signal peptide retained the fusion protein in the cytoplasm (Fig. 3g).

Increased grain yield of are1 under low nitrogen conditions

Data presented above indicate that are1 is an important mediator of NUE. To assess the potential contribution of ARE1 to grain yield, we performed a multi-year field trial to analyze the major agronomic traits of are1-1 plants grown under various nitrogen conditions, in comparison with the wild-type NPB variety. The experiment was repeated for four years (2013–2016) and each sample included at least three duplicates. Under our assay condition, the saturated nitrogen fertilizer concentrations are approximately 280–300 kg/ha urea, similar to the routine usage by farmers in most rice-growing areas in China¹⁴.

During early growth stages, no substantial difference was observed between NPB and are1-1 plants. However, after heading, are1-1 showed significantly delayed senescence than NPB under various nitrogen conditions (Fig. 4a and Supplementary Fig. 5a), accompanying with increased photosynthetic activities (Fig. 4b) and higher nitrogen utilization efficiency (Fig. 2e, f and Supplementary Table 1). Compared to that of NPB, although the plant height and the tiller number of are1-1 were marginally altered, the panicle length and the numbers of primary and secondary branches of panicles were increased in are1-1, resulting in an increase in grain number per panicle (Fig. 4c, d and Supplementary Fig. 5b–f). However, one-thousand-grain weight of are1-1 was slightly reduced than that of NPB (Fig. 4e). Overall, the grain yield of are1-1 plants increased by 10–20% than NPB grown under the nitrogen-limiting condition (40–60% of the saturated concentration; Fig. 4f and Supplementary Fig. 5g, h), under which conditions the differences of NUpE and NUE between NPB and are1-1 were also most apparent (Fig. 2e, f and Supplementary Table 1). Most notably, the grain yield of are1-1 grown under the nitrogen-limiting condition was comparable to that of NPB grown under the saturated urea condition (Fig. 4f and Supplementary Fig. 5g, h). These results indicate that are1-1 is a beneficial allele of high NUE and is particularly useful under nitrogen-limiting growth conditions.

Analysis of genetic variations in ARE1

Given that ARE1 plays an important role in mediating NUE and grain yield, we then explored potential association between these traits and genetic variations in ARE1. By analyzing a panel of Asian cultivated rice accessions from the 3000 Rice Genomes Project^15,16, multiple variations were identified in the ARE1 promoters and coding sequences (Fig. 5a, b). In a panel of 2747 accessions, 15 single nucleotide polymorphisms (SNPs) were identified in the coding region, which were classified as 12 major haplotypes (Fig. 5b). Among these 15 SNPs, five variations caused substitutions in the coded amino acid residues, of which all occurred at very low frequencies (0.07–2.33%; Fig. 5b). The functional significance of these five SNPs remains elusive. In 2155 accessions with available data in the promoter region, three major haplotypes, ARE1^NPB, ARE1⁹³¹¹, and ARE1^MH63, were identified, represented by the NPB, 9311 and Minghui63 (MH63) promoters, respectively. In these three haplotypes, three insertion–deletion polymorphisms (InDels) were identified in the promoter region of ARE1, of which the ARE1⁹³¹¹ and ARE1^MH63 haplotypes contained two small insertions of 6-bp at different positions (Fig. 5a).

Because of relatively more dramatic alterations in the genome structure, we focused on the analysis of the InDel polymorphisms in the ARE1 promoter. We found that the majority of japonica varieties were the ARE1^NPB haplotype, with only 2.00, 3.50, and 4.46% occurrence of the ARE1⁹³¹¹/ARE1^MH63 alleles in the ancient japonica, temperate japonica, and tropical japonica accessions, respectively (Fig. 5c). However, the occurrence of the ARE1⁹³¹¹/ARE1^MH63 alleles was increased to 18.84% in the examined 1449 indica accessions, and higher frequency of the ARE1⁹³¹¹/ARE1^MH63 alleles was observed in aus (48.13%) and aromatic (100%) (Fig. 5c), suggesting that the ARE1 locus has been likely subjected to artificial selection during breeding.

Genetic variations modulate ARE1 expression and grain yield

To evaluate the biological function of these InDels, we examined the ARE1 expression levels in representative indica and japonica accessions of different haplotypes. All the analyzed ARE1⁹³¹¹/ARE1^MH63 haplotypes, regardless of their genetic backgrounds, had a decreased expression level of ARE1 than the ARE1^NPB haplotypes under high nitrogen growth condition (Fig. 6a). Moreover, whereas low nitrogen treatment repressed the ARE1 expression, the ARE1^NPB haplotypes were more sensitive to low nitrogen than the ARE1⁹³¹¹/ARE1^MH63 haplotypes (Fig. 6a). This result suggests that the ARE1 expression is negatively regulated by nitrogen availability and the genetic variations in the promoter of the ARE1⁹³¹¹/ARE1^MH63 accessions cause the reduced promoter activity and the responsiveness to nitrogen. Correlated to these results, the differences of grain yield between high and low nitrogen growth conditions in accessions carrying an ARE1^NPB allele were generally greater than that of those carrying an ARE1⁹³¹¹/ARE1^MH63 allele (Fig. 6b and Supplementary Fig. 6a), suggesting that the two insertions in the ARE1⁹³¹¹ and ARE1^MH63 haplotypes are negatively correlated to both the ARE1 expression level and grain yield.

To further test whether these InDels are functional in a similar genetic background, we analyzed the phenotypes of a set of recombinant inbred lines (RILs) generated from crosses between two indica rice varieties 9311 and Peiai64 (PA64), also known as Liangyoupei9 (LYP9), a widely cultivated high-yield hybrid rice¹⁷. Of these two parents, PA64 was characterized as an ARE1^NPB haplotype. In a panel of 134 RILs of LYP9, the ARE1⁹³¹¹ InDel was tightly co-segregated with the phenotype of the reduced ARE1 expression level (Fig. 6c), which, in turn, was negatively correlated to the grain yield under nitrogen-limiting conditions (Fig. 6d and Supplementary Fig. 6b).

In a transgenic study, overexpression of ARE1 caused the reduction in grain yield (Supplementary Fig. 6c, e, f). Conversely, the knockdown of the ARE1 expression by RNAi caused an increased grain yield under nitrogen-limited conditions (Supplementary Fig. 6d, g, h), indicating that the expression level of ARE1 is directly and negatively correlated to the grain yield.

Indica rice varieties generally have higher NUE than japonica varieties^18,19,20. We next asked if the are1-1 allele, which is in the japonica variety NPB genetic background, was also advantageous for high-yield indica varieties. In a near-isogenic line of MH63 carrying an are1-1 allele (MH63^are1-1), the photosynthetic activity was substantially higher than its parent MH63, accompanying with an increase in the grain number and, eventually, an increase in grain yield (Fig. 6e and Supplementary Fig. 6i, j). Taken together, these results suggest that the genetic variations in the ARE1 promoter directly regulate its expression, thereby the rice productivity under nitrogen-limiting conditions.

Discussion

In this study, we have presented multiple lines of evidence demonstrating that ARE1 is a key determinant of the grain yield by modulating NUE in rice. Loss-of-function mutations in ARE1 largely rescue the nitrogen deficient syndrome caused by impaired nitrogen assimilation associated with the reduced Fd-GOGAT/ABC1 activity^10,11, indicating that ARE1 is a negative regulator of nitrogen assimilation. ARE1 is a chloroplast-localized protein with unknown function. Along with its subcellular localization, the enhanced photosynthetic activity and delayed-senescence phenotype of are1 mutants imply that ARE1 is not only involved in nitrogen assimilation, but may also be related to carbon metabolism. Consistent with this notion, the homologous gene of ARE1 in algae is implied in regulating photosynthesis by modulating CO₂ transport^21,22,23. ARE1 shares approximately 27% homology with cotA protein of Synechocystis PCC6803 (see Supplementary Fig. 4a), which is also localized in chloroplasts. Mutations in the cotA gene caused reduced CO₂ transport activity, presumably resulting from defects in light-induced proton extrusion^21,22. In Chlamydomonas reinhardtii, the chloroplast-localized Ycf10 protein, which shares homology with cotA, has been shown to promote inorganic carbon (CO₂ and HCO₃^-) uptake into chloroplasts²³. Although the molecular mechanism of cotA-mediated and Ycf10-mediated inorganic carbon transport remains elusive, a similar biochemical activity may also be utilized by ARE1-like proteins in higher plants to modulate photosynthesis, which provide the carbon skeletons, energy and reductants for nitrogen metabolism. Understanding the precise biochemical function of ARE1 will be crucial to reveal the mechanism of ARE1-mediated carbon and nitrogen metabolism. Because the GS/Fd-GOGAT cycle is a key converging point of C/N balance that is directly linked to the TCA cycle^24,25, a key question to be addressed is the biochemical and molecular mechanisms of the repressing effect of ARE1 on the Fd-GOGAT activity.

Genetically, a suppressor may act upstream or downstream of the modified locus. Because are1-1 partially rescued the phenotype of the weak allele abc1-1, but not that of the null allele abc1-2, the ARE1 function should be dependent on ABC1. Therefore, ARE1 may genetically act upstream of Fd-GOGAT/ABC1. In agreement with this notion, whereas gogat1, which carries a substitution mutation in Fd-GOGAT/ABC1, shows an early senescence phenotype and an increase in grain nitrogen deposition¹¹, are1-1 shows an opposite phenotype, further suggesting that ABC1 and ARE1 act in a linear genetic pathway. Moreover, we notice that the ARE1 expression level is substantially increased in the rice grain number, plant height, and heading date7 (ghd7) mutant^26,27, indicating that the expression of ARE1 is negatively regulated by Ghd7. As implied from its name, Ghd7 regulates multiple traits, including the grain number and chlorophyll contents^26,27,28, which are also affected by the are1 mutations. We speculated that Ghd7 may act genetically upstream to regulate the ARE1 expression, thereby modulating a subset of similar physiological and developmental processes.

The structure of the ARE1 promoter shows significant variations among a large pool of rice varieties. The two small insertions in the ARE1 promoter are rarely found in all japonica varieties, but the occurrence is significantly increased in indica and aus accessions, implying that these genetic variations may have been artificially selected during breeding. We speculate that these two InDels were selected in some indica and aus varieties under certain circumstances, likely using the grain number per panicle as a selectable trait. This notion may explain the high frequency of these two InDels in aus, a class of varieties that are widely grown in the south Asia areas where the nitrogen deposition in soil is significantly lowered than other regions^29,30,31. Finally, when grown under nitrogen-limiting conditions, the grain yield of both japonica and indica varieties carrying an are1-1 allele is comparable to that of their parents supplied with the saturated amount of nitrogen fertilizers. Therefore, discoveries made in this study present a potential solution of reducing the application of nitrogen fertilizers in rice production. Because of the conservation of ARE1-like genes, a similar approach may also be applicable to other crops.

Methods

Plant materials and growth conditions

The abc1-1 mutant is a weak mutant allele carrying a single substitution mutation in Fd-GOGAT/ABC1 in the Nipponbare background, and the abc1-2 mutant is a T-DNA insertion mutant (PFG_3A-01082) in the Dongjin background¹⁰. The abc1-1 seeds were mutagenized by ethyl methanesulfonate and M₂ seeds (approximately 28800 seeds) obtained from self-pollinated M₁ plants were used for screening of putative suppressors under field growth conditions. The screen was performed by mainly analyzing of the plant height, tiller numbers and leaf color during the primary round of screen. Putative are mutants were subjected to the secondary round of screen in the M₃ generation and then backcrossed with wild-type (Nipponbare; NPB) or abc1-1 mutant plants for at least three times. The MH63^are1 plants were constructed by backcrossing of are1-1 with MH63 for seven times, and resulting BC₆F₄ population were used in this study. The LYP9 recombinant inbred lines (RILs) were generated from a cross between an indica variety 9311 and a maternal indica variety PA64, a photo-thermosensitive male sterile line¹⁷. The inbred F₁₃ generation was used in this study.

Plants were grown in paddy fields in Beijing, Jinan (Shandong province), Hangzhou (Zhejiang province), and Lingshui (Hainan province) with routine management. For hydroponic grown seedlings, seeds were surface-sterilized with 75% ethanol for 5 min and then sterilized with 30% bleach for 30 min, followed by washing with sterile water for six times. Sterilized seeds were germinated in distilled water for 36–48 h at 37 °C in darkness, and then transferred to a modified hydroponic culture solution³² and fresh solution was changed every 3 days. The modified hydroponic culture solution contains variable concentrations of NH₄NO₃ (0, 0.15, 0.73, or 1.46 mM), 0.37 mM CaCl₂, 0.55 mM MgSO₄, 0.18 mM KH₂PO₄, 0.09 mM K₂SO₄, 46.26 μM H₃BO₃, 0.32 μM CuSO₄, 0.77 μM ZnSO₄, 9.15 μM MnCl₂, 0.38 μM K₂MoO₄, 20 μM Fe-EDTA, and 0.36 mM Na₂SiO₃, pH5.5. When transferring between hydroponic culture solutions containing different concentrations of NH₄NO₃, seedlings were washed in distill water for 3–5 times. Rice seedlings were cultured in a greenhouse or a growth chamber with 70% relative humidity under 12 h/12 h light/dark and 25–30 °C/25 °C day/night conditions.

Genetic transformation of rice was performed as described^33,34. Brifly, rice embryonic calli were infected with agrobacteria cells for 1–2 min, followed by extensive washing with sterile water. The infected calli were cultured on N6-AS medium (3.981 g/L N6 salts, 2 mg/L 2,4-D, 0.3 g/L casein hydrolysate, 30 g/L sucrose, 10 g/L glucose, 15 mg/L acetosyringone, 4 g/L phytagel, pH 5.2) for 3 days at 25 ^oC and then washed with sterile water containing 500 mg/L carbenicillin for three times. The calli were cultured on selection medium (3.981 g/L N6 salts, 2 mg/L 2,4-D, 0.5 g/L casein hydrolysate, 0.5 g/L l-proline, 30 g/L sucrose, 4 g/L phytagel, 200 mg/L carbenicillin, 200 mg/L cefotaxime, 50 mg/L hygromycin B, pH 5.8) for 4 weeks by changing fresh selection medium once. Putative positive transformants (hygromycin B-resistant calli) were then transferred to differentiation medium (4.4 g/L MS salts, 30 g/L sucrose, 30 g/L sorbitol, 2 g/L casein hydrolysate, 0.02 mg/L NAA, 2 mg/L kinetin, 4 g/L phytagel, 50 mg/L carbenicillin, 50 mg/L hygromycin B, pH 5.8) and cultured for 4–8 weeks. The regenerated plantlets were cultured on rooting medium (2.2 g/L MS salts, 0.5 g/L L-proline, 0.5 g/L casein hydrolysate, 30 g/L sucrose, 0.5 mg/L IBA, 4 g/L phytagel, pH 5.8) for several weeks and then grown in soil in a greenhouse upon rooting.

Field trials

A randomized block design approach was used to analyze the major agronomic traits under various nitrogen conditions. Rice plants were cultivated with a distance of 15 × 25 cm in a 3.8–4.0 × 4.0 m plot and each sample or treatment included six replicates (plots). Urea was used as the only nitrogen source and applied for three times at the seedling, tillering and booting stages, with 30, 40, and 30% of total applied urea at each stage, respectively. Potassium sulfate (120 kg/ha) and calcium phosphate (260 kg/ha) were used as phosphorus and potassium fertilizers, supplied when rice seedling transplanting.

Plasmid construction

Plasmids were constructed following standard methods as described³⁵. To construct pARE1::ARE1, three partially overlapped DNA fragments (5000, 2000, and 3227 bp, respectively) were PCR-amplified using NPB genomic DNA as templates and primers embedded with appropriate restriction sites. The PCR fragments were first cloned into a pBluescript SK (Stratagene) vector and then sequentially inserted into the PstI/SacI sites of a binary vector pCAMBIA1300.

To construct p35S::ARE1-FLAG plasmid, a 279-bp NOS terminator fragment was PCR-amplified and then inserted into the SacI/EcoRI sites of pCAMBIA1300 to generate a pCAMBIA1300-NOS vector. A 1295-bp fragment was PCR-amplified using NPB cDNA as a template and cloned into the PstI/XmaI sites of pBluescript SK, in-frame fused to a FLAG tag to generate an ARE1-FLAG fusion gene, which was then released by PstI/XbaI and inserted into the PstI/XbaI sites of pCAMBIA1300-NOS to generate pARE1-FLAG-NOS. A 35S promoter fragment was PCR-amplified and then inserted into the PstI site of pARE1-FLAG-NOS to generate p35S::ARE1-FLAG.

To construct pUbi::RNAi-ARE1, a 324-bp fragment (nucleotides 101–400; the first nucleotide of the putative translation codon was referred to as +1) was PCR-amplified using NPB cDNA as a template and then inserted into the pTCK303 vector in an inverted orientation³⁶.

To construct p35S::ARE1-YFP, a 1296-bp fragment was PCR-amplified using NPB cDNA as a template and then inserted into HindIII/SalI sites of pSAT6-EYFP-N1 vector³⁷. The p35S::ARE1^Δ1-47-YFP vector was constructed using a similar approach.

To construct pARE1::GUS, the GUS gene was inserted into BamHI/SacI sites of pCAMBIA1300-NOS vector to generate pCAMBIA1300-GUS-NOS. A 2.3-kb DNA fragment spanning the putative ARE1 promoter region (−2276 to +6; the putative transcription start is referred to as +1) was PCR-amplified using NPB genomic DNA as a template. The PCR fragment was digested with PstI (embedded in the PCR primers) and then inserted into the PstI site of pCAMBIA1300-GUS-NOS vector.

All constructs were verified by DNA sequencing analysis and extensive restriction digestion. Sequences of all primers used in cloning are listed in Supplementary Table 2. All binary vector-based plasmids were transformed into Agrobacterium tumefaciens strain EHA105 by electroporation and the resulting bacteria cultures were used for the transformation of rice.

Map-based cloning

F₂ mapping populations were generated by crossing abc1-1 are1-1 (in the japonica NPB background) with indica varieties MH63 and Nanjing6, respectively. F₂ segregants showing rescued leaf chlorosis of abc1-1 were selected for genetic mapping. Using 170 recombinants, the ARE1 locus was tentatively mapped to an interval between two SSR markers, RM3374, and RM3481, on the short arm of chromosome 8. Using additional 1233 recombinants, ARE1 was mapped to a 410-kb region between two newly developed SNP markers M79 and M81. The ARE1 candidate gene was identified by DNA sequencing analysis. Sequences of all primers used in genetic mapping are listed in Supplementary Table 2.

Reverse transcription-quantitative PCR analysis

Total RNA from various organs was extracted using RNAprep Pure Plant Kit (TIANGEN, Beijing, China). Total RNA (~2 μg) was treated with DNase I for 10 min at room temperature, and then used for cDNA synthesis using TransScript^® First-Strand cDNA Synthesis SuperMix (TransGen Biotech, Beijing, China) according to the manufacturer’s instructions. Quantitative PCR experiments were performed with gene-specific primers using UltraSYBR Mixture (CWBIO, Beijing, China) in a CFX96^TM Real-Time System (BIO-RAD, US). The rice Ubiquitin (UBQ) gene was used as an internal control for normalization. Sequences of all primers used in qPCR are listed in Supplementary Table 2.

Analysis of GUS activity

The GUS (β-glucuronidase) reporter activity was assayed by histochemical staining as described³⁸. Briefly, Various tissues/organs derived from pARE1::GUS transgenic plants at the seedling stage (seedling), the jointing-booting stage (leaf sheath, leaf blade, and panicle) or the heading stage (spike) were collected and incubated in 90% acetone for 15 min at 4 °C. After washing three times with GUS staining solution [10 mM EDTA, 78.4 mM Na₂HPO₄, 31.6 mM NaH₂PO₄, 0.5 mM K₄Fe(CN)₆, 0.5 mM K₃Fe(CN)₆, 0.1% Triton X-100, and 20 mM 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-Gluc)] and evacuation, samples were incubated in the staining solution for 2–24 h at 37 °C in darkness. The samples were destained by dehydration with 70% ethanol and then photographed.

Immunoblotting analysis

The preparation of protein extracts and immunoblotting analysis were performed as described previously^10,34. Leaves were grinded into fine powder in liquid nitrogen and total proteins were extracted in cold extraction buffer (50 mM HEPES, pH 7.5, 15 mM KCl, 1 mM EDTA, 1 mM DTT, and 1 mM PMSF) at 4 °C. The sample was cleaned by centrifugation at 20000×g at 4 °C for 10 min twice and the supernatant was collected. The sample was mixed with equal volume of 2× loading buffer (100 mM Tris–Cl, pH 6.8, 1.0% β-mercaptoethanol, 0.2% bromophenol blue, 20% glycerol, 4% sodium dodecyl sulfate), boiled for 1–2 min, and then subjected to SDS–PAGE. After gel electrophoresis, the proteins were electrically transferred onto a polyvinylidene difluoride membrane and analyzed by immunoblotting. A rabbit anti-Fd-GOGAT antibody (Agrisera; Cat #: AS07242; 1:20000 dilution) and a mouse anti-HSP82 antibody (Beijing Protein Innovation; Cat #: AbM51099-31-PU; 1:10000 dilution) were used as primary antibodies and HPR-conjugated goat anti-rabbit IgG or HPR-conjugated goat anti-mouse IgG (Dingguo Changsheng Biotech, Beijing, China; 1:50000 dilution) were used as secondary antibodies. The signal was detected using a SuperSignal West Femto Maximun Sensitivity Substrate kit (Thermo Scientific, Cat #: 34096) according to the manufacturer’s instructions.

Transient expression assays in rice protoplasts

Rice protoplasts were prepared as described with minor modification³⁹. Briefly, stem and sheath tissues derived from 14-day-old rice seedlings were cut into 0.5–1.0 mm strips. The sample was immediately transferred into 150 mL of 0.6 M mannitol and incubated for 10 min, followed by incubation in an enzyme solution (0.6 M mannitol, 10 mM MES, pH 5.7, 10 mM CaCl₂, 5 nM β-mercaptoethanol, 1.5% cellulase RS, 0.75% macerozyme R-10, 0.1% BSA, and 50 μg/mL carbenicillin) for ~4 h at 28 °C with gentle shaking (40–60 rpm) in darkness. Protoplasts were collected using a 35 μm mesh filter and resuspended in one volume of W5 solution (154 mM NaCl, 125 mM CaCl₂, 5 mM KCl, and 2 mM MES, pH 5.7) by inverting for 5–8 times, followed by centrifugation at 150×g for 3 min. Protoplasts were then resuspended in MMG solution (0.6 M mannitol, 15 mM MgCl₂, and 4 mM MES, pH 5.7) at a concentration of 1–5 × 10⁶ cells/mL. The viability of protoplasts was determined by the FDA staining method as described⁴⁰.

For transient expression assays, 5 μg plasmid DNA were introduced into 50 μL freshly prepared protoplasts by PEG-mediated transformation as described⁴¹. Briefly, 55 μL freshly prepared PEG solution (0.6 M mannitol, 100 mM CaCl₂ and 40% PEG4000) was gently added, and then incubated for 15 min at 28 °C in darkness, followed by gently mixed with ten volumes of W5 solution and inverted for 5–8 times. The protoplasts were pelleted by centrifugation at 150×g for 3 min, and then gently resuspended in W5 solution.

For the analysis of subcellular localization, after transformation with appropriate expression vectors, protoplasts were cultured for 12–16 h in darkness at 28 °C, and then fluorescence signals were visualized and scanned under a confocal laser scanning microscope (Leica TCS SP5). At least 50 cells were analyzed in each sample.

Analysis of Fd-GOGAT enzyme activity

Analysis of Fd-GOGAT enzyme activity was performed as described^10,42 with minor modifications. Briefly, plant extracts were prepared by grinding 2 g fresh leaves of 3-week-old seedlings in 1 mL of cold extraction buffer (50 mM HEPES, pH 7.5, 15 mM KCl, 1 mM EDTA, 1 mM DTT, and 1 mM PMSF) at 4 °C, and then cleared by centrifugation at 20000×g for 10 min at 4 °C, followed by collection of the supernatants on ice. To assay Fd-GOGAT activity, 200 μL plant extracts (~240 μg protein) were mixed with 800 μL reaction mixture [50 mM HEPES, pH 8.5, 1% (v/v) β-mercaptoethanol, 3.65 mM glutamine, 3 mM α-oxoglutarate, 0.2 mM NADPH, and 0 or 4 μM ferredoxin], and then transferred into a quartz cuvette incubated in a DU^® 800 Nucleic Acid/Protein Analyzer (Beckman Coulter, US) for the Kinetics/Time Run. The Fd-GOGAT activity [nmoles NADPH oxidized (37 °C, pH 8.5)/min/mg protein] was measured spectrophotometrically by recording the rate of NADPH oxidation at 340 nm (indicated by a change in A₃₄₀ nm blanked using reaction mixture without the addition of NADPH), and corrected by the rate measured from the reaction mixture without ferredoxin. All experiments were repeated at least three times (biological replicates), and mean values of these replicates are presented.

Determination of total chlorophyll content and SPAD

To measure total chlorophyll contents, 10–15 mg leaves was collected in 2 mL 96% ethanol, and then homogenized in a MM 400 grinding apparatus (Retsch, Germany), followed by incubation at 4 °C for 2 h. The chlorophyll content was calculated spectrophotometrically based on the absorbance of the supernatant at 649 and 665 nm as described⁴³. The relative chlorophyll content in leaves of plants grown in the paddy field was determined by a soil plant analysis development (SPAD) value measured by an SPAD-502 Plus chlorophyll meter (Konica Minolta, Japan) following the manufacturer’s instructions.

Determination of total nitrogen content

To measure total nitrogen contents, samples were incubated in a drying oven at 105 °C for 30 min, followed by incubation at 90 °C for 5 days. Dry weights were measured as biomass values, and total nitrogen of various organs was determined by the Kjeldahl method as described^32,44. The nitrogen uptake efficiency (NUpE) was determined by the ratio of total aboveground plant nitrogen to applied fertilizer nitrogen, and the nitrogen use efficiency (NUE) was determined by the ratio of total grain yield to applied fertilizer nitrogen, respectively, as described⁴⁵.

Bioinformatics analysis

Prediction of the subcellular localization and cleavage site of ARE1 protein were used TargetP 1.1 Server (http://www.cbs.dtu.dk/services/TargetP/) with cutoff value 0.500 according to the online instructions⁴⁶. Prediction of transmembrane helices in ARE1 protein was performed via TMHMM 2.0 Server (http://www.cbs.dtu.dk/services/TMHMM/). Construction of the phylogenetic tree was performed using MEGA 5.10 software by Neighbor-Jointing method with 1000 bootstrapping trails.

Data availability

The authors declare that all data supporting the findings of this study are available within the manuscript or its supplementary files or are available from the corresponding authors upon request.

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Acknowledgements

We thank Dr. Kang Chong for providing vectors; Drs. Kang Chong, Chengcai Chu, Zhizhong Gong, Shuhua Yang, and members of Zuo lab for helpful discussion. This study was supported by grants from Chinese Academy of Sciences (XDA08010401-2 and XDPB04), the Ministry of Science and Technology of China (2016YFD0100706 and 2014CB943402), and the State Key Laboratory of Plant Genomics.

Author information

Qing Wang, Jinqiang Nian and Xianzhi Xie contributed equally to this work.

Authors and Affiliations

State Key Laboratory of Plant Genomics and National Plant Gene Research Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China
Qing Wang, Jinqiang Nian, Hong Yu, Jian Zhang, Jiaoteng Bai, Lichao Chen, Qingjun Xie, Jian Feng, Xiaolu Yang, Juli Peng, Jiayang Li & Jianru Zuo
University of Chinese Academy of Sciences, 100049, Beijing, China
Qing Wang, Jiaoteng Bai, Lichao Chen, Qingjun Xie, Jian Feng, Xiaolu Yang, Fan Chen, Jiayang Li & Jianru Zuo
Shandong Rice Research Institute, Shandong Academy of Agricultural Sciences, 250100, Jinan, China
Xianzhi Xie & Bo Bai
State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, 310006, Hangzhou, China
Guojun Dong, Jiang Hu & Qian Qian
State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China
Fan Chen
Chinese Academy of Sciences Center for Excellence in Molecular Plant Sciences, 200032, Shanghai, China
Jianru Zuo

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Qing Wang
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Contributions

J.Zuo., J.L., Q.Q., Q.W., X.X., and F.C. designed the experiments and analyzed the data. Q.W., J.N., and X.X. performed most experiments, with assistance of J.Zhang, J.B., G.D., J.H., B.B., L.C., Q.X., J.F., X.Y., and J.P. H.Y. and Q.W. performed the genome sequence analyses. J.Zuo. and Q.W. wrote the paper. All authors read and commented on the paper.

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Correspondence to Qian Qian, Jiayang Li or Jianru Zuo.

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Wang, Q., Nian, J., Xie, X. et al. Genetic variations in ARE1 mediate grain yield by modulating nitrogen utilization in rice. Nat Commun 9, 735 (2018). https://doi.org/10.1038/s41467-017-02781-w

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Received: 10 July 2017
Accepted: 22 December 2017
Published: 21 February 2018
DOI: https://doi.org/10.1038/s41467-017-02781-w

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