Abstract
The loss of lipid homeostasis can lead to lipid overload and is associated with a variety of disease states. However, little is known as to how the disruption of lipid regulation or lipid overload affects cell survival. In this study we investigated how excess diacylglycerol (DG), a cardinal metabolite suspected to mediate lipotoxicity, compromises the survival of yeast cells. We reveal that increased DG achieved by either genetic manipulation or pharmacological administration of 1,2-dioctanoyl-sn-glycerol (DOG) triggers necrotic cell death. The toxic effects of DG are linked to glucose metabolism and require a functional Rim101 signaling cascade involving the Rim21-dependent sensing complex and the activation of a calpain-like protease. The Rim101 cascade is an established pathway that triggers a transcriptional response to alkaline or lipid stress. We propose that the Rim101 pathway senses DG-induced lipid perturbation and conducts a signaling response that either facilitates cellular adaptation or triggers lipotoxic cell death. Using established models of lipotoxicity, i.e., high-fat diet in Drosophila and palmitic acid administration in cultured human endothelial cells, we present evidence that the core mechanism underlying this calpain-dependent lipotoxic cell death pathway is phylogenetically conserved.
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Introduction
The maintenance of precise lipid compositions is crucial to guarantee membrane integrity, proper signaling, and trafficking. Most eukaryotic organelle membranes consist of phosphatidylcholine (PC), phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidic acid (PA), diacylglycerol (DG), sterols, and sphingolipids. These lipids differ in their characteristics regarding bilayer formation, curvature determination, regulation of fission, and fusion processes, and membrane protein embedding [1]. How cells regulate and maintain the lipid composition of membranes is not yet fully understood but is a crucial requirement to facilitate their diverse functions.
Lipid overload can lead to cellular lipotoxicity, which in higher eukaryotes can trigger tissue degeneration, precipitating a number of diseases, including metabolic syndrome, type II diabetes mellitus, cardiovascular disorders, hepatosteatosis, and cancer [2, 3]. The lipid species which are most relevant for lipotoxicity are under discussion, but most probably include free fatty acids (FFA), ceramide, cholesterol, and DG [3,4,5,6]. Although evidence for the lipotoxic nature of these lipids exists, the exact mechanisms underlying lipotoxic cell death remain unclear [7].
DG is a central intermediate in the synthesis of membrane phospholipids and the storage lipid, triacylglycerol (TG), and its cellular steady state levels are typically very low. De-regulated DG levels, on the other hand, are suspected to be involved in the development of insulin resistance and diabetes [8], and its abundance correlates with the occurrence of non-alcoholic fatty liver disease, including steatosis, steatohepatitis and cirrhosis [6]. An inherent problem of these studies, however, is that the regulation of DG takes place at multiple anabolic and catabolic levels and in various subcellular compartments. Given that, experimental manipulation of DG concentrations is an extremely difficult task. The different DG pools within subcellular compartments such as the endoplasmic reticulum (ER), lipid droplets or plasma membrane, their metabolic origins (TG synthesis, TG lipolysis, and phospholipid turnover) and regio isomerism (sn-1,2-, sn-1,3-, or sn-2,3-) of DG need to be considered for its biological activity and metabolic fate [9]. Given the experimental restrictions in higher eukaryotes, we decided to study DG toxicity in the budding yeast Saccharomyces cerevisiae, since its genetics offer the unique possibility to cut off all DG-catabolizing pathways. Using two different strategies to accumulate DG in yeast, we found that increased levels of DG are sufficient to induce cell death and identified glucose repression and Rim101 signaling as crucial mediators of DG lipotoxicity. Additional experimental evidence obtained using established lipotoxicity models in Drosophila and a human endothelial cell line suggest that the core of this lipotoxicity pathway is evolutionary conserved in metazoans.
Results
A genetically engineered yeast strain accumulates DG
To increase cellular DG levels, we generated an S. cerevisiae triple knockout strain (TKO), which accumulates endogenous DG. This was achieved by deleting genes of three DG-metabolizing enzymes: (i) DGA1, (ii) LRO1—both encoding DG-acyl transferases catalyzing the last step of TG formation—and (iii) DGK1, responsible for the phosphorylation of DG to PA (Fig. 1a). A third major DG-consuming reaction via the Kennedy pathway was modulated by the addition (or omission) of choline [10], which drains DG into the synthesis of PC (Fig. 1a).
Mass spectrometric lipid analysis revealed that, as expected, the TKO (dga1∆ lro1∆ dgk1∆) contained increased intracellular DG levels (Fig. 1b, c), while levels of TG (Fig. 1d) were decreased. Interestingly, a double-knockout (DKO) mutant deleted for DGA1 and LRO1 genes also displayed a moderate but significant increase in DG (Fig. 1b, c) allowing us to comparatively analyze different DG levels by using either the DKO or the TKO strains. Thin layer chromatography revealed that the accumulating DG species had sn-1,2 configuration, consistent with their origin from de novo synthesis (Fig. 1e).
DG accumulation leads to increased necrotic cell death and ROS production
The toxic effects of DG accumulation could be observed by a significant decrease in growth in the TKO strain that was partly restored by administration of 1 mM choline chloride (Fig. 2a). A time course monitoring clonogenic survival upon DG accumulation showed that the severity of inflicted lipotoxicity was proportional to DG levels (Fig. 2b). This reduction in survival was accompanied by the overproduction of reactive oxygen species (ROS), as determined by the ROS/superoxide-dependent conversion of non-fluorescent dihydroethidium (DHE) to fluorescent ethidium (Fig. 2c, d). As ROS production often precedes programmed cell death we made use of an AnnexinV/PI assay to differentiate between apoptotic and necrotic cell death [11]. In this assay, apoptosis is represented by the AnnexinV-positive fraction, whereas secondary necrosis and primary necrosis are represented by the AnnexinV/PI-double-positive or PI-only-positive fractions, respectively [11, 12]. The PI-only-positive fraction was predominant in DKO and TKO cells suggesting a necrotic type of cell death (Fig. 2e, f).
We next compared the survival of DKO, TKO and wild-type strains using different carbon sources. Interestingly, cell death was only triggered upon growth on glucose-containing media (Fig. 3a) while ROS accumulation and necrotic cell death were significantly reduced when glucose was replaced with galactose (Fig. 3b–e). In summary, our data suggest that an increase in cellular DG induces glucose-dependent and ROS-associated necrotic cell death in yeast.
Administration of a small, cell permeable DG analog triggers glucose-dependent necrosis in yeast
We made use of the small, cell permeable DG analog 1,2-dioctanoyl-sn-glycerol (DOG) as a complementary approach to increase cellular DG in yeast. DOG administration has been previously used to mimic endogenous DG in the fission yeast Schizosaccharomyces pombe [13] and in mammalian cells [14] for investigating both protein kinase C-dependent and independent roles of DG. Importantly, external DOG administration to wild-type yeast cultures led to the induction of cell death (Fig. 4a), which was accompanied by the accumulation of ROS (Fig. 4b). In order to test whether the production of ROS was causally linked to cell death induction, we made use of the ROS scavenger N-acetyl cysteine [15], which we administered to the yeast cultures. Our results reveal that ROS scavenging only shows limited potential in preventing cell death in both our model systems of DAG-induced cell death (Supplementary Fig. 1a, b). Interestingly, the effects of DOG treatment were limited to cells cultured in glucose medium as growth on galactose (Fig. 4a, b) and raffinose (data not shown) entirely prevented DOG-induced cell death and ROS accumulation. ROS accumulation and cell death were detectable after 14 h of DOG treatment when cells usually begin to exhaust glucose and enter the diauxic shift phase of growth. We hypothesized that the metabolic changes that occur due to glucose depletion would be responsible for cell death induction by DOG. We therefore conducted a series of experiments, in which we shifted DOG-exposed cells onto spent media. Indeed, exposure to spent media led to a rapid decrease in survival (Supplementary Fig. 1c) that was attributable to necrotic cell death (Supplementary Fig. 1d). This cell death could be prevented by glucose supplementation (Supplementary Fig. 1e, f).
DOG-induced lipotoxicity depends on glucose repression and de-repression
Glucose repression has been extensively studied in yeast and is known to regulate a number of genes [16]. We conducted a screen to investigate whether glucose repression is actively involved in DOG-mediated cell death (Supplementary Fig. 1g). Intriguingly, we found that the master regulator of glucose repression, hexokinase 2 (Hxk2), is a crucial factor for DOG-mediated cell death, whereas hexokinase 1, which is not involved in glucose repression, is dispensable for this effect (Fig. 4e). Of note, HXK2 deletion in the TKO background resulted in a similar outcome in that survival was significantly increased whereas ROS accumulation was substantially decreased (Fig. 4f, g).
The regulatory function of Hxk2 with respect to glucose repression can be separated from its enzymatic activity as hexokinase [17]. Phosphorylation of Hxk2 at serine 14 regulates its localization to either the cytoplasm or nucleus, and determines its role in glucose repression [18]. To assess whether abrogation of the regulatory function of Hxk2 was sufficient to prevent cell death, we made use of two Hxk2 mutants, one in which serine 14 residue was replaced by alanine (S14A) and another in which serine 14 was changed to aspartate (S14D). The non-phosphorylatable Hxk2S14A mutant is found in the cytoplasm and nucleus and constitutively activates glucose repression, while the phospho-mimetic Hxk2S14D is localized to the cytosol and fails to activate glucose repression. In line with our hypothesis, expression of Hxk2S14D suppressed DOG-induced cell death (Fig. 4f, g). However, the rescue of cell death was unexpected for the S14A mutation, as our initial hypothesis pictured glucose repression as being crucial and committing for cell death induction. The results from the S14A experiment rather suggested that constant glucose repression was not sufficient to trigger cell death. This led us to conclude that sequential de-repression after glucose repression is required for DOG-induced cell death.
DOG-induced cell death depends on the calpain-like cysteine protease Rim13
In order to further understand the mechanistic details of how yeast cells die in response to DG, we applied DOG treatment to all deletion mutants of known and putative yeast programmed cell death (PCD) regulators [19,20,21,22,23]. This screen revealed that the yeast calpain-like protease Rim13 (alternatively called Cpl1) is essential for DOG-mediated lipotoxicity (Fig. 5a). Cells lacking Rim13 were completely resistant to DOG-mediated toxicity, as determined by PI staining (Fig. 5a). Importantly, the deletion of known regulators of yeast apoptosis such as YCA1 [20] and AIF1 [22] did not influence the DOG-induced cellular demise. Mass spectrometric analysis confirmed that administered DOG readily entered the yeast cells, intracellular DG levels were not affected by RIM13 deletion and DOG treatment substantially increased total cellular DG levels (Fig. 5b, c, Supplementary Fig. 2a).
Deletion of RIM13 also significantly reduced ROS accumulation and cell death rates upon DOG treatment (Fig. 5d–f, Supplementary Fig. 2b, c). Deletion of RIM13 in the TKO background led to increased survival (Fig. 5g) and reduced ROS accumulation (Fig. 5h), further suggesting that RIM13 is crucial for triggering DG-induced cell death. Importantly, RIM13 deletion did not affect lipid levels in the TKO strain (Supplementary Fig. 3). To discriminate between apoptosis and necrosis in the DOG treatment setting, we performed an AnnexinV/PI assay. The increase in necrotic cells that is usually observed when DOG was added to wild-type yeast cells was lost upon deletion of RIM13 (Fig. 5i, Supplementary Fig. 4).
DOG-triggered PCD requires Rim13 cysteine protease activity, its carboxy-terminal domain and depends on Rim101 processing
Next we attempted to complement the lack of RIM13 in the knock out strain by expression of RIM13 on a plasmid that is under control of its endogenous promoter. To verify whether cell death depends on the proteolytic activity of Rim13, a point mutation in which cysteine128 of the active site was exchanged for alanine was also generated. As expected, the DOG-resistance of the rim13∆ strain was reverted when RIM13 was reintroduced (Fig. 6a). ROS production was also restored to wild-type levels in the rim13∆ strain upon re-expressing RIM13 (Fig. 6b). This rescue was not observed upon replacement of endogenous RIM13 by the rim13C128A mutant allele, indicating that the cysteine protease function of Rim13 is crucial for cell death (Fig. 6a, b). A similar complementation assay in the rim13∆ strain revealed that its carboxy-terminal domain is also required for cell death (Fig. 6c, d). This domain has previously been linked to the regulation of Rim13 localization and Rim13 mediated cleavage of the transcriptional repressor Rim101 [24]. RIM101 deletion itself was also protective against DOG-mediated cell death induction (Fig. 6e) and ROS accumulation (Fig. 6f). Importantly, expression of a carboxy-terminally truncated Rim101 version (Rim1011-531), which is constitutively active [25], re-established DOG-mediated cell death in the rim13∆ strain (Fig. 6g). Rim101 activation through manipulation of plasma membrane lipids has been reported to depend on the Rim21 sensor complex which includes RIM21, RIM9, DFG16, and RIM8 [26, 27]. This raised the question whether DG-induced cell death would also depend on this sensor complex. We addressed this question in such a way that we subjected all the single knock outs of the sensor complex (rim21∆, rim9∆, dfg16∆, and rim8∆) to DOG treatment and assessed the survival by clonogenic survival assay and PI staining. Our results reveal that the sensor complex is indeed crucial to fully conduct the lipotoxic response (Fig. 6h, i). To further investigate this in our genetic model of DG increase, we knocked out RIM21 in the TKO background and measured the impact on survival. The obtained data (Fig. 6j and Supplementary Fig. 5a) confirm that Rim21 also has a functional role in this setting of DG-induced cell death suggesting that DG-mediated activation of the Rim101 pathway actively involves the Rim21 sensor complex.
In summary, our yeast data provide evidence for the existence of a lipotoxic cell death pathway that can be triggered by excess DG (Supplementary Fig. 5). This pathway is dependent on functional glucose repression and de-repression and requires Rim13-mediated activation of the transcriptional repressor Rim101.
The calpain-dependent lipotoxic cell death pathway is evolutionary conserved
To investigate whether the lipotoxic cell death pathway that we identified in yeast is evolutionarily conserved across metazoa, we decided to test two well-established models of lipotoxicity in higher organisms: palmitic acid stress applied to mammalian cell culture and coconut oil rich high-fat diet (HFD) fed Drosophila melanogaster. Importantly the central regulator of lipotoxic cell death that we have identified in yeast, Rim13, is phylogenetically conserved (Fig. 7a). Palmitic acid increases cytosolic Ca2+ levels and induces programmed necrosis in endothelial cells [28]. Small interfering RNA (siRNA)-mediated knockdown of m-calpain-1 (CAPN1), the human orthologue of RIM13 (Fig. S6a) rendered cells resistant to palmitic acid stress (Fig. 7b, c), as evidenced by increased viability (Fig. 7b) and reduction of cell death markers (Fig. 7c). Importantly the levels of basal cytosolic Ca2+ were not affected upon CAPN1 knockdown (Fig. 7d). Palmitic acid stress has been described to increase DG levels in H9C2 cardiomyoblasts [29] which we could confirm in a lipidomic approach using our endothelial cell line (Fig. 7e, Supplementary Fig. 6b). Further analysis of the DG species revealed that the total DG increase is mostly due to palmitate incorporation into DG as documented by the 32:0 species, which harbors two palmitate residues (Fig. 7f). Interestingly, DG accumulation was not dependent on calpain 1 as no reversion of this phenomenon was observed with CAPN1 knockdown cells (Fig. 7e, f). We thus suggest that palmitic acid-induced cell death is mediated through DG accumulation and that calpain acts downstream of this event.
Feeding a coconut-based HFD to Drosophila leads to adverse effects such as body fat increase, reduced activity, cardiomyopathy and a reduced lifespan [30, 31]. Drosophila has four Calp genes, two of which (CalpA and CalpB) encode proteolytically active calpains [32]. We thus generated a ubiquitous CalpA+B double gene knockdown (CalpA+B DKD) and compared the lifespans on HFD with the RNAi turned on or off. Importantly, reduction of CalpA and CalpB expression to 25% of the normal level (Fig. 7g) increased the median lifespan on HFD by 21% and the maximum lifespan by 33% (Fig. 7h). The bodyweight of DKD flies was not reduced with respect to controls, excluding a potential dietary restriction-like effect (Supplementary Fig. 6c). In a nutshell, these findings suggest that calpains act as key regulators for organismal lipotoxicity.
Discussion
In fungi the Rim101 pathway constitutes a well conserved signal transduction route that is primarily known for sensing and reacting to pH alteration [33, 34]. Importantly, it is required for the pathogenicity of Candida albicans, Cryptococcus species and other noxious fungi [35]. However, the Rim101 pathway not only senses alkaline conditions, but also recognizes lipid alterations in the plasma membrane such as changes in the asymmetrical lipid distribution among the two leaflets of the bilayer [26, 36, 37]. The Rim101 pathway includes a sophisticated sensor complex consisting of Rim8 and the three transmembrane proteins Dfg16, Rim9 and Rim21. The carboxy-terminal cytosolic domain of Rim21 localizes to the plasma membrane under normal conditions, whereas lipid perturbation of the plasma membrane triggers its dissociation [38]. The sensor complex then induces a downstream proteolytical complex, which consists of Rim13, Rim20, Ygr122w, and Rim101 [33]. The cysteine protease Rim13 is the sole yeast orthologue of mammalian calpains, explaining its alternative denomination as Cpl1 (Calpain-like protease) [39]. Rim13 proteolytically cleaves and thereby activates the transcriptional repressor Rim101 [39]. Most processes of the Rim101 cascade have been studied as a response to pH stress. Induction of the Rim101 pathway as a result of lipid stress at the plasma membrane has been investigated by means of genetic deletion of flippases and floppases or their upstream regulators [26, 38, 40], by addition of palmitoleic acid [41] and by expressing phospholipase A2 [42]. In most of these studies, it appears that Rim21 senses changes in the plasma membrane´s lipid asymmetry in a similar way as under alkaline conditions, since both conditions interfere with the charge gradient present at the plasma membrane. Hence, disturbance of specific physicochemical properties of the plasma membrane could be perceived by the sensing complex involving Rim21 under both conditions of stress. Carboxy-terminal cleavage of Rim101 by Rim13 uncovers its gene repressing activity. A number of Rim101-repressed genes have been identified by the Mitchell lab so far and include NRG1, PRB1, RIM8, SMP1, YJR061W, YOR389W, and YPL277C, the promoters of which directly interact with Rim101 [43]. Nrg1 and Smp1 themselves represent transcription factors on a second level and contribute to the large complexity of Rim101 controlled gene regulation.
Activation of the Rim101 pathway is the natural response to alkaline or lipid stress in yeast that is presumably activated with the scope of rearranging the lipid composition of the plasma membrane and hence to facilitate adaptation to environmental changes. Here we have shown that, in the case of non-physiological elevation of DG, this particular stress response is triggering a necrotic type of lipotoxic cell death rather than an adaptive response (Figs. 2, 5). Our results reveal that two transcriptional regulatory pathways (i) glucose repression and (ii) the Rim101 pathway including its Rim21-dependent sensing complex are involved in DG-mediated lipotoxicity. This raised the question whether these two pathways interfere with cell death regulation on the same level, culminating in a similar outcome or whether they represent distinct routes. Interestingly, the transcriptional repressor Nrg1 lies at the crossroads of glucose repression and the Rim101 pathway and could thus represent such a downstream regulator integrating signals from these different routes. Nrg1 controls repression of the SUC2 and GAL genes, which are needed for growth on alternative carbon sources, as well as for haploid invasive growth and diploid pseudohyphal differentiation [44]. Nrg1 further represses the expression of the sphingoid long-chain base efflux transporter Rsb1 [45, 46]. Rsb1 in turn upregulates Lem3 which controls the flippases Dnf1 and Dnf2 and negatively regulates the floppase Yor1, possibly explaining the lipid stress-induced changes of the plasma membrane [41]. Smp1, the other transcriptional repressor which is regulated by Rim101, controls rough colony morphology, sporulation, and haploid invasive growth [43]. Altogether, this suggests that excess DG activates the Rim101 pathway that integrates its signal with the glucose repression pathway on the level of transcriptional repressors and thereby regulates cell fate decisions. We hypothesize that these decisions can include adaptation to the initial stress or the initiation of regulated cell death [47].
The production of ROS could potentially be regulated by glucose repression and Rim101 signaling, both pathways of which merge at the level of NRG1. Nrg1 further regulates the repression of genes involved in alternative carbon source usage as mentioned above [16]. This might actually affect respiration and electron transport efficiency within respiratory complexes and thus impact ROS production. However, since the effects of ROS scavenging cannot fully account for an essential role in DG-induced necrosis (Supplementary Fig. 1a, b) we rather think that ROS accumulate as a matter of altered lipid metabolism or as part of a failed cellular attempt to prevent cell death. The fact that DG-induced cell death depends on glucose repression and de-repression highlights that the metabolic state of cells is important to make them susceptible to cell death induction. After the diauxic shift, when toxic effects are starting to become visible, the cells have an increased demand for lipid mobilization for example to feed into mitochondrial membrane expansion to allow for efficient respiration. This could explain for the sudden toxicity of DG arising after the metabolic shift. Further research will need to be conducted to fully answer these interesting questions.
Intriguingly, calpains have been identified as crucial regulators of necrotic cell death in diverse models of neurodegeneration [48, 49]. The lipotoxic models we used in Fig. 7 suggest that calpain-mediated lipotoxic cell death might have developed rather early during evolution and that its core function as a regulator of lipotoxic cell death is conserved across species. Calpain-mediated lipotoxicity could further be important for lipid-associated disease or during aging and neurodegeneration, where metabolic changes are also prevalent. Calpain might have evolved as a regulator of lipid metabolism to sense and react to changes in the lipid environment. Our lipotoxic models are suitable to further investigate the underlying mechanism which will be addressed in further studies.
Methods
Yeast strains and growth conditions
All experiments were carried out in the BY4741 (MATa his3∆1 leu2∆0 met15∆0 ura3∆0) strain background. Single deletion strains were obtained from the EUROSCARF knock out collection except for the self-generated rim13∆ strains in Supplementary Fig. 1. The dga1∆ lro1∆ DKO was generated in a previous study [50]. The dga1∆ lro1∆ dgk1∆ TKO was generated in the DKO background by using the URA3 gene disruption cassette, which was subsequently excised by Cre-recombinase expression to regain the ura3 marker [51]. The same technique was used to generate four independent cpl1∆ strains, which all yielded the same phenotypes as the EUROSCARF rim13∆ strain. Strains were grown in SC medium containing 0.17% yeast nitrogen base (Difco), 0.5% (NH4)2SO4 and 30 mg/l of all amino acids (except 80 mg/l histidine and 200 mg/l leucine), 30 mg/l adenine, and 320 mg/l uracil with 2% glucose as carbon source for SCD medium or 2% galactose for SCG medium, respectively. All yeast cultures were inoculated from a stationary overnight culture to an OD600 = 0.1 and then grown at 28 °C and 145 rpm shaking for indicated time periods. For DOG stress experiments, cultures were stressed with 1.45 mM DOG (1,2-dioctanoyl-sn-glycerol, Cayman) at an OD600 = 0.35. For ROS scavenging experiments N-acetylcysteine (NAC) was added to a final concentration of 10, 30 and 100 mM at 14 h after DOG stress or 14 h after inoculation for TKO experiments.
Cloning and molecular biology
RIM13/CPL1 was cloned into plasmid pRS313 under its endogenous promoter using the NotI and EcoRI restriction sites. The C128A mutation was introduced using two-step PCR assisted site-directed mutagenesis [52]. The oligonucleotides used for cloning are listed in Supplementary Table 1.
Survival plating and test for apoptotic/necrotic markers
For survival plating, the cell concentrations of culture dilutions were determined with a CASY cell counter (Schaerfe Systems) and aliquots containing 500 cells were plated on YPD plates. The number of colonies formed was determined after 2 days at 28 °C. AnnexinV/PI co-staining was performed as previously described [11]. 30,000 cells were evaluated using flow cytometry and analyzed using BD FACSDiva software. For dihydroethidium staining, 5 × 106 cells were harvested by centrifugation, resuspended in 250 μl of 2.5 μg/ml DHE in PBS, and incubated in the dark for 5 min. Relative fluorescence units (RFU) were determined using a fluorescence reader (Tecan, GeniusPRO) and then normalized to an OD600 of 0.2. For ROS analysis based on individual cells flow cytometry was used to count positive cells. The same samples were analyzed by fluorescence microscopy on a Zeiss Axioskop microscope equipped with a rhodamine filter set.
Lipid analysis and mass spectrometry in yeast
After addition of 50 µl of an internal standard mix (Supplementary Table 2) to each sample, total lipids were extracted from exponentially growing yeast cultures (harvested 12 h after inoculation) with chloroform/methanol 2:1 (v/v) according to Folch et al. [53]. The organic phase was dried under a stream of nitrogen, and dissolved in 500 μl of chloroform/methanol (2:1, v/v).
Analysis of lipid extracts was carried out by an ACQUITY-UPLC system (Waters, Manchester, UK) equipped with a BEH C18 column (2.1 × 150 mm, 1.7 µm, Waters) coupled to a SYNAPT G1 qTOF HD mass spectrometer (Waters) [54]. Separation of lipids was achieved with a binary gradient consisting of solvent A water/methanol (1:1, v/v) and solvent B isopropanol. Each solvent contained 8 µM phosphoric acid, 10 mM ammonium acetate and 0.1 Vol% formic acid. The gradient started at 45% solvent B and was increased to 90% B within 30 min. Thereafter, the eluent was raised to 100% B within 2 min and kept for another 10 min. The system was changed back to starting conditions within 1 min, and the column was equilibrated for 7 min prior to the next analysis. The column was kept at 50 °C in a column oven. Ionization was achieved by an ESI source using the following parameters: capillary temperature: 100 °C, desolvatization temperature: 400 °C, N2 as nebulizer gas. The capillary voltage was set to 2.1 kV in negative mode and 2.6 kV in positive mode. Injection of the sample ranged from 5–10 µl depending on the used ionization mode (pos/neg). For positive mode the samples were diluted 1:5 with isopropanol whereas for negative mode samples were dissolved in a mixture of isopropanol and chloroform/methanol 2:1 (9:1, v/v) and injected without prior dilution. Leucine enkephaline (Sigma-Aldrich) at a concentration of 100 pg/µl in water/acetonitrile (1:1, v/v)+0.1 Vol% formic acid as a lock mass reference was supplied by an external pump (L-6200 intelligent pump, Hitachi) at a flow rate of 0.2 ml/min splitted in a 1:13 ratio. For data acquisition MSE scan mode was applied to generate full- and MS/MS scans. Data were analyzed by the MassLynx 4.1 (Waters) and by “Lipid Data Analyzer” software [55].
Neutral lipid separation and analysis was performed by thin layer chromatography (TLC) on silica gel plates (Merck), essentially as described before [56], using chloroform/acetone/acetic acid (90:8:1, per vol) as solvent. TLC plates were dipped into 3.2% H2SO4 and 0.5% MnCl2 followed by carbonization at 120 °C for 30 min and photometric scanning in a CAMAG TLC scanner.
Cell culture, annexinV/PI staining and siRNA-mediated gene silencing
Endothelial cells from the human umbilical vein endothelial cell-derived cell line EA.hy926 were used in this study. Cells were grown in DMEM containing 10% FCS, 1% HAT (5 mM hypoxanthin, 20 μM aminopterin and 0.8 mM thymidine), 50 units/ml penicillin, 50 μg/ml streptomycin at 37 °C in 5% CO2 atmosphere.
For microscopic analysis endothelial cells grown on glass coverslips were transfected with calpain 1 (CAPN1; siRNA from Qiagen (SI02757314)) or scrambled control siRNA at 60 percent confluence and were treated with 0.5 mM palmitic acid complexed to bovine serum albumin (BSA) in a ratio of 6:1 for 16 h or BSA (control) after 48 h of transfection. Cells were stained with AnnexinV-Fluos staining kit from Roche Biodiagnostics (Roche Diagnostics GmbH). According to the manufacturers protocol 20 μl of AnnexinV-Fluos were diluted in 1 ml of incubation buffer and 20 μl of propidium iodide (PI) was added. 100 μl of this mixture were added directly to the culture and cells were analyzed after 20 min of incubation.
High-resolution imaging of AnnexinV/ PI was performed using an array confocal laser scanning microscope (ACLSM) as described previously [28] using a 40× oil objective. Cells were selected randomly on wide field and then excited with 488 and 515 nm simultaneously for AnnexinV and PI fluorescence, respectively. Images were captured by a charged-coupled device (CCD) camera (CoolSNAP-HQ, Photometrics, Tucson, USA). All devices were controlled by VisiView Premier acquisition software (Visitron Systems, Puchheim, Germany). Cytosolic Ca2+ measurement: For cytosolic Ca2+ measurements the Fura-2 technique was used as described previously [28].
Lipid extraction from endothelial cells and quantification by shotgun mass spectrometry
Cell pellets were homogenized with 1 mm zirconia beads in a cooled tissuelyzer for 2 × 5 min at 30 Hz in 300 µl IPA. An aliquot of the homogenate was used for protein determination by BCA assay and 50 µg of total protein were used for lipid extraction [57, 58]. In brief, 700 µl internal standard mix in 10:3 methyl tert-butyl ether/methanol was added to each sample and vortexed for 1 h at 4 °C. After addition of 140 µl H2O samples were vortexed for another 15 min. Phase separation was induced by centrifugation at 13,400 r.p.m. for 15 min. The organic phase was transferred to a glass vial and evaporated. Samples were reconstituted in 300 µl methanol/chloroform (1/2 per vol). For lipidome and PS measurements 5 µl of sample were diluted with 95 µl isopropoanol/ methanol/chloroform (4/2/1 per vol)+7.5 mM ammonium formate and ethanol/chloroform (4/1 per vol)+0.1% trimethylamine, respectively.
Mass spectrometric analysis was performed on a Q Exactive instrument (Thermo Fisher Scientific, Bremen, DE) equipped with a robotic nanoflow ion source TriVersa NanoMate (Advion BioSciences, Ithaca, USA) using nanoelectrospray chips with a diameter of 4.1 μm. The ion source was controlled by the Chipsoft 8.3.1 software (Advion BioSciences). Ionization voltage was +0.96 kV in positive and −0.96 kV in negative mode; back pressure was set at 1.25 psi in both modes. Samples were analyzed by polarity switching [58]. The temperature of the ion transfer capillary was 200 °C; S-lens RF level was set to 50%. Each sample was analyzed for 5.7 min. FT-MS spectra were acquired within the range of m/z 400–1000 from 0 min to 1.5 min in positive and within the range of m/z 350–1000 from 4.2 min to 5.7 min in negative mode at a mass resolution of R m/z 200 = 140,000, automated gain control (AGC) of 3 × 106 and with an maximal injection time of 3000 ms. PS was additionally measured for 1.5 min in neg FT-MS mode with the same parameters as mentioned above. All acquired spectra were filtered by PeakStrainer (https://git.mpi-cbg.de/labShevchenko/PeakStrainer/wikis/home) [59]. Lipids were identified by LipidXplorer software [60]. Molecular Fragmentation Query Language (MFQL) queries were compiled for PC, PCO, LPC, PE, PEO, LPE, PI, LPI, Cer, PA, LPA, PG, PS, TG, DG lipid classes. The identification relied on accurately determined intact lipid masses (mass accuracy better than 5 p.p.m.) and signal to noise threshold higher than 3. Lipids were quantified by comparing the isotopically corrected abundances of their molecular ions with the abundances of internal standards of the same lipid class.
Standards for lipid quantification
Synthetic lipid standards were purchased from Avanti Polar Lipids, Inc. (Alabaster, USA). All used solvents were of at least HPLC grade. Stocks of internal standards were stored in glass ampoules at −20 °C until used for the preparation of internal standard mix in MTBE/methanol (10/3 per vol). 700 µl internal standard mix contained: 1040 pmol cholesteryl ester 16:0 D7, 521 pmol 50:0 TG D5, 145 pmol 34:0 DG D5, 550 pmol 25:0 PC, 435 pmol LPC, 107 pmol 25:0 PS, 295 pmol 25:0 PE, 85 pmol 13:0 LPE, 192 pmol 25:0 PI, 109 pmol 25:0 PG, 73 pmol 30:1 Cer, 123 pmol 25:0 PA, 91 pmol 13:0 LPA, 32 pmol 13:0 LPI.
Fly techniques
The y[1] w*; P{w[+mC] = Act5C-GAL4}25FO1/CyO, y+(short name: Act5C-Gal4) fly stock (BDSC #4414) is available from the Bloomington Drosophila Stock Center. The transgenic RNAi fly lines, which allow conditional gene knockdown of the CalpA (P{KK104532}VIE-260B; short name CalpA KD; VDRC #101294) and CalpB (w[1118]; P{GD16349}v46241/TM3; short name CalpB KD; VDRC #46241) genes, respectively as well as the w[1118] mutant control line (VDRC #60000) were acquired from the Vienna Drosophila RNAi Center. RNAi effector transgene flies, which allow CalpA and CalpB double-knockdown are represented by the fly stock w*; P{KK104532}VIE-260B; P{GD16349}v46241 / TM3 Sb*, P{w[+] Ubx-lacZ} (short name CalpA+B DKD). All flies were propagated at 25 °C with a 12 h:12 h light/dark cycle on a complex malt-soy flour-corn flour-molasses standard food (SD) as described before [41].
RNA extraction and assessment of RNAi-mediated Calp gene knockdown
Ten flies per sample were disintegrated and homogenized followed by Trizol extraction. The homogenate was extracted once with chloroform and the RNA was precipitated form the supernatant with isopropanol and washed with 70% ethanol. 500 ng RNA were treated with DNaseI followed by reverse transcription using Superscript III Reverse Transcriptase (Invitrogen Inc.). For quantitative real time PCR Invitrogen´s SYBR Select Master Mix was used. CalpA and CalpB gene expression levels of male flies subject to ubiquitous CalpA+B DKD double knockdown (y1 w*; P{w[+mC] = Act5C-GAL4}25FO1/P{KK104532}VIE-260B; P{GD16349}v46241 /+) were compared to controls (y1 w*; P{w[+mC] = Act5C-GAL4}25FO1/+). qRT-PCR was performed using the primers listed in Table S1 and CalpA and CalpB expression was normalized to RpL32 gene expression levels to assess the efficiency of the knock downs.
Lifespan assay
Lifespan assays were performed as described before [41]. In brief, n = 120 age-matched male flies (12 replicates of cohorts of 10 flies) for each genotype were raised on SD until hatching to adult flies. Animals were kept at 23 °C with a 12 h:12 h light/dark cycle on high-fat diet (HFD) food, which was prepared by adding 30 ml coconut oil (Sigma) to 100 ml of SD. The HFD food was changed every 3–4 days and the dead flies were scored and removed from the experiment at indicated time points. Data plotted in Fig. 7f show a representative of two experiments, which displays age-specific average survivorship rate means calculated from the replicates. Maximal lifespan values correspond to 10% survivorship. A log-rank test was performed to assess statistical significance of the observed differences between the CalpA+B DKD flies and the control flies survivorship data. Average fly wet-weight was determined by weighing cohorts of 10 flies each.
Statistical analysis
Statistical analyses were calculated in Prism7. For assessment of significance one-way, two-way or three-way ANOVA were performed as indicated. Error bars indicate SEM and asterisks in the figures indicate significant differences, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
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Acknowledgements
P.R. is an Erwin Schrödinger research fellow of the Austrian Science Fund FWF (project J3742 B28). F.M. is grateful to the Austrian Science Fund FWF (Austria) for grants P23490-B20, P29262, P24381, P29203 P27893, I1000 and ‘SFB Lipotox’ (F3012), as well as to BMWFW and Karl-Franzens University for grant ‘Unkonventionelle Forschung’ and grant DKplus Metabolic and Cardiovascular Diseases (W1226). S.D.K. acknowledges support by FWF for projects SFB Lipotox (F3005) and DK Molecular Enzymology (project W901-B12). We acknowledge support from NAWI Graz and the BioTechMed-Graz flagship project “EPIAge”. R.P.K was supported by the Max Planck Society. O.K. was a member of the PhD program ‘Molecular Enzymology’, funded by the FWF (project W901-B12) and M.J.K. was a member of the PhD program ‘Metabolic and Cardiovascular Disease’ funded by the FWF (project W1226-B18). The authors thank Regina Krügener for technical assistance. The Bloomington Drosophila Stock Center (BDSC; supported by NIH P40OD018537) and the Vienna Drosophila Resource Center (VDRC) are acknowledged for providing fly stocks. We are grateful to Dr. C.J.S. Edgell (University of North Carolina, Chapel Hill, NC, USA) for the EA.hy926 cells. Plasmids expressing carboxy-terminally truncated Rim13 variants were thankfully obtained from Aaron P. Mitchell, pRS316-Rim1011-531 was a kind gift from Olivier Vincent and the authors thank Fernando Moreno for providing the hexokinase 2 mutants.
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Rockenfeller, P., Smolnig, M., Diessl, J. et al. Diacylglycerol triggers Rim101 pathway–dependent necrosis in yeast: a model for lipotoxicity. Cell Death Differ 25, 767–783 (2018). https://doi.org/10.1038/s41418-017-0014-2
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DOI: https://doi.org/10.1038/s41418-017-0014-2