Abstract
Various inhibitors of histone deacetylase (HDAC) activity can sensitize drug resistant cancer cells to chemotherapeutic agents. However, the mechanisms underlying such effects of distinct HDAC inhibitors (HDACi) remain poorly understood. Here we show that both the HDACi trichostatin A and valproic acid induced a sensitization of multidrug-resistant cancer cells to the topoisomerase II inhibitor etoposide/VP16. This effect was associated with increased acetylation of certain lysines on histones H3 and H4, including lysine 16 on histone H4 (H4K16). Overexpression of the histone acetyltransferase hMOF, known to target H4K16, was sufficient to mimic HDACi treatment on sensitization and H4K16 acetylation, and importantly, small-interfering RNA (siRNA)-mediated knockdown of hMOF abolished the HDACi-mediated sensitizing effects as well as the increase in H4K16 acetylation. Conversely, siRNA-mediated knockdown of the H4K16 deacetylase SIRT1 mimicked HDACi treatment whereas overexpression of SIRT1 abolished H4K16 acetylation and significantly reduced the sensitizing effects of HDACi. Interestingly, the effects of hMOF on H4K16 acetylation and sensitization to the topoisomerase II inhibitor could be directly counteracted by exogenous expression of increasing amounts of SIRT1 and vice versa. Our study results suggest that hMOF and SIRT1 activities are critical parameters in HDACi-mediated sensitization of multidrug-resistant cancer cells to topoisomerase II inhibitor and increased H4K16 acetylation.
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Abbreviations
- EX-527:
-
6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide;
- DAPI:
-
4′,6-diamidino-2-phenylindole
- VP16:
-
etoposide
- GFP:
-
green fluorescent protein
- HAT:
-
histone acetyltransferase
- HDAC:
-
histone deacetylase
- HDACi:
-
HDAC inhibitors
- H4K16:
-
lysine 16 on histone H4
- MOF:
-
males absent on the first
- NSCLC:
-
non-small-cell lung carcinoma
- siRNA:
-
small-interfering RNA
- TSA:
-
trichostatin A
- VPA:
-
valproic acid
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Acknowledgements
We thank RG Roeder (The Rockefeller University), L Guarente (Massachusetts Institute of Technology) and M Henriksson (Karolinska Institutet) for providing us with hMOF DNA construct, SIRT1 DNA construct and SK-N-BE(2) cell line, respectively, and S Orrenius, T Perlmann and members of Hermanson and Joseph's labs for helpful comments. This work was supported by the Swedish Research Council, the Swedish Cancer Society, the Åke Wiberg Foundation, the Swedish Medical Society, the Karolinska Institutet Foundations (KI Cancer) (BJ and OH), SSF (CEDB & OBOE) and the Swedish Children's Cancer Foundation (OH).
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Hajji, N., Wallenborg, K., Vlachos, P. et al. Opposing effects of hMOF and SIRT1 on H4K16 acetylation and the sensitivity to the topoisomerase II inhibitor etoposide. Oncogene 29, 2192–2204 (2010). https://doi.org/10.1038/onc.2009.505
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DOI: https://doi.org/10.1038/onc.2009.505
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