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Interaction between the keratin cytoskeleton and eEF1Bγ affects protein synthesis in epithelial cells

Nature Structural & Molecular Biology volume 14, pages 982–983 (2007)Cite this article

Abstract

Eukaryotic elongation factor-1 (eEF1) is essential for peptide-chain elongation during translation. We report that its γ subunit (eEF1Bγ) specifically binds, and bundles, keratin intermediate filaments. Disrupting this interaction depresses translation by ∼20% and selectively increases 80S ribosomes in epithelial cells, an outcome recapitulated by RNA interference–mediated silencing of eEF1Bγ. These findings extend the emerging relationship between keratin proteins and the translational machinery.

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Figure 1: eEF1Bγ bundles keratin IFs.
Figure 2: Disrupting the keratin-eEF1Bγ185–223 interaction and silencing eEF1Bγ depress bulk protein translation similarly.

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Acknowledgements

We thank J. Lorsch, C. Parent, T. Kinzy, J. Dijk, D. Robinson and Y. Zheng for help. This work was supported by US National Institutes of Health grants AR42047 and AR44232 to P.A.C.

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Authors and Affiliations

  1. Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, 21205, Maryland, USA

    Seyun Kim, Juliane Kellner, Chang-Hun Lee & Pierre A Coulombe

  2. Department of Dermatology, The Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, 21205, Maryland, USA

    Pierre A Coulombe

Authors
  1. Seyun Kim
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  2. Juliane Kellner
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  3. Chang-Hun Lee
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  4. Pierre A Coulombe
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Correspondence to Pierre A Coulombe.

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Kim, S., Kellner, J., Lee, CH. et al. Interaction between the keratin cytoskeleton and eEF1Bγ affects protein synthesis in epithelial cells. Nat Struct Mol Biol 14, 982–983 (2007). https://doi.org/10.1038/nsmb1301

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