Abstract
While next generation sequencing technology can produce sequences covering the entire genome, assembly and annotation are still prohibitive steps for many phylogenomics applications. Here we describe a method of Target Restricted Assembly (TRAM) of a single lane of Illumina sequences for genes of relevance to phylogeny reconstruction, i.e. single copy protein-coding genes. This method has the potential to produce a data set of hundreds of genes using only one Illumina lane per taxon.
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Johnson, K., Walden, K. & Robertson, H. A Target Restricted Assembly Method (TRAM) for Phylogenomics. Nat Prec (2010). https://doi.org/10.1038/npre.2010.4612.1
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DOI: https://doi.org/10.1038/npre.2010.4612.1