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Gene targeting with nucleases: capped templates, semper fidelis?

Nature Methods volume 11, pages 1029–1030 (2014)Cite this article

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Use of adenoviral vectors to deliver donor templates for genome editing facilitates precise genome modifications in human cells. This has implications for both basic and translational applications of rare-cleaving nuclease technologies.

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References

  1. Segal, D.J. & Meckler, J.F. Annu. Rev. Genomics Hum. Genet. 14, 135–158 (2013).

    Article  CAS  Google Scholar 

  2. Voytas, D.F. Annu. Rev. Plant Biol. 64, 327–350 (2013).

    Article  CAS  Google Scholar 

  3. Kim, H. & Kim, J.-S. Nat. Rev. Genet. 15, 321–334 (2014).

    Article  CAS  Google Scholar 

  4. Esvelt, K.M. & Wang, H.H. Mol. Syst. Biol. 9, 641 (2013).

    Article  Google Scholar 

  5. Holkers, M. et al. Nat. Methods 11, 1051–1057 (2014).

    Article  CAS  Google Scholar 

  6. Anguela, X.M. et al. Blood 122, 3283–3287 (2013).

    Article  CAS  Google Scholar 

  7. Li, H. et al. Nature 475, 217–221 (2011).

    Article  CAS  Google Scholar 

  8. Perez-Pinera, P., Ousterout, D.G., Brown, M.T. & Gersbach, C.A. Nucleic Acids Res. 40, 3741–3752 (2012).

    Article  CAS  Google Scholar 

  9. Beard, B.C., Adair, J.E., Trobridge, G.D. & Kiem, H.-P. Methods Mol. Biol. 1185, 321–344 (2014).

    Article  Google Scholar 

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  1. Andrew Scharenberg is at Seattle Children's Research Institute, Seattle, Washington, USA.,

    Andrew Scharenberg

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  1. Andrew Scharenberg
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Correspondence to Andrew Scharenberg.

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Yes there is potential Competing Interest.

I have equity in Bluebird Bio. I am on the Bluebird Bio Scientific Advisory Board, and consult for Bluebird Bio.

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Scharenberg, A. Gene targeting with nucleases: capped templates, semper fidelis?. Nat Methods 11, 1029–1030 (2014). https://doi.org/10.1038/nmeth.3110

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