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Enhanced Y1H assays for Arabidopsis

Nature Methods volume 8, pages 1053–1055 (2011)Cite this article

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Abstract

We present an Arabidopsis thaliana full-length transcription factor resource of 92% of root stele–expressed transcription factors and 74.5% of root-expressed transcription factors. We demonstrate its use with enhanced yeast one-hybrid (eY1H) screening for rapid, systematic mapping of plant transcription factor–promoter interactions. We identified 158 interactions with 13 stele-expressed promoters, many of which occur physically or are regulatory in planta.

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Figure 1: An Arabidopsis eY1H transcription factor resource.
Figure 2: Using the transcription factor resource in an eY1H assay.

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Acknowledgements

This work was supported by University of California Davis startup funds to S.M.B., US National Institutes of Health grant GM082971 to A.J.M.W., US Department of Agriculture 1907-21000-030 to D.W., GM092412 and GM056006 to S.A.K., We thank K. Cavanaugh, D. Lavelle, L. Williams, P. Nittler, R. Michelmore, J. Pelletier, K. Vo, J. Foret, S. Winte and J. Harada for assistance.

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Authors and Affiliations

  1. Department of Plant Biology and Genome Center, University of California Davis, Davis, California, USA

    Allison Gaudinier, Mallorie Taylor-Teeples, Li Pu, Dahae Kim & Siobhan M Brady

  2. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA

    Lifang Zhang, Zhijie Liu & Doreen Ware

  3. Program in Gene Function and Expression and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA

    John S Reece-Hoyes & Albertha J M Walhout

  4. Division of Biological Sciences, Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California, USA

    Ghislain Breton, Jose L Pruneda-Paz & Steve A Kay

  5. US Department of Agriculture, Agricultural Research Service, Ithaca, New York, USA

    Doreen Ware

Authors
  1. Allison Gaudinier
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  11. Albertha J M Walhout
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  13. Siobhan M Brady
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Contributions

S.M.B., D.W., A.J.M.W. and J.S.R.-H. conceived the project; J.S.R.-H. and A.J.M.W. provided the eY1H mating protocol; A.G. and D.K. performed the yeast experiments; A.G. and L.Z. cloned the de novo transcription factors; L.Z., M.T.-T., A.G., L.P. and Z.L. recombined the transcription factors; L.Z., G.B., J.L.P.-P. and S.A.K. accumulated and curated transcription factor entry clones; and S.M.B., A.G., D.W., L.Z., A.J.M.W. and J.S.R.-H. wrote the manuscript.

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Correspondence to Siobhan M Brady.

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The authors declare no competing financial interests.

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Supplementary Figures 1–3, Supplementary Tables 1–3 and Supplementary Notes (PDF 888 kb)

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Gaudinier, A., Zhang, L., Reece-Hoyes, J. et al. Enhanced Y1H assays for Arabidopsis. Nat Methods 8, 1053–1055 (2011). https://doi.org/10.1038/nmeth.1750

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