Abstract
Strategies for altering constitutional or somatic genotype in mice are well established, but approaches to generate mosaic genotypes in mouse tissues are limited. We showed that a functionally inactive Cre recombinase transgene with a long mononucleotide tract altering the reading frame was stochastically activated in the mouse intestinal tract. We demonstrated the utility of this approach by inducing colonic polyposis after Cre-mediated bi-allelic inactivation of the Apc gene.
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Acknowledgements
We thank T. Saunders and the University of Michigan Transgenic Core for outstanding support of the transgenic studies, the University of Michigan Microscopy and Image Analysis Laboratory for assistance with microscopy, S. Camper (University of Michigan) for providing R26R mice and the caggB plasmid, S. Tarle for technical assistance, and K.R. Cho for helpful discussions. This work was supported by US National Institutes of Health grants CA082223, CA085463 and GM067840.
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A.A., T.H., Y.F. and G.T.B. performed experiments. T.M.G. provided key reagents. E.R.F. supervised the work and wrote the manuscript.
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Akyol, A., Hinoi, T., Feng, Y. et al. Generating somatic mosaicism with a Cre recombinase–microsatellite sequence transgene. Nat Methods 5, 231–233 (2008). https://doi.org/10.1038/nmeth.1182
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DOI: https://doi.org/10.1038/nmeth.1182
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