Increased Activity of Choline Acetyl-transferase in Sympathetic Ganglia after Prolonged Administration of Nerve Growth Factor

Abstract

A prolonged increase in the activity of preganglionic sympathetic nerves produces characteristic changes in the enzyme pattern of the postganglionic adrenergic neurones in adult rats^1–4. In newborn mice the normal development of adrenergic neurones depends materially on the intactness of the preganglionic cholinergic nerves⁵. These two findings contribute to the definition of the so far vague term “trophic response to neuronal activity” and the question arises whether these trophic actions are unidirectional only, or whether there is also a retrograde effect, that is, a dependence of the preganglionic cholinergic nerves on the function of the postjunctional adrenergic neurones. Transection experiments in the brain have shown that after lesioning of particular areas, for example, optic nerve tract, cingulate or visual cortex, there occurs not only orthograde but also retrograde trans-synaptic degeneration⁶. It seemed of interest to study this possible retrograde trophic effect in a less complex system and to investigate whether there are not only negative (degeneration) but also positive effects. The administration of nerve growth factor (NGF) to newborn rats produces a marked growth and enhanced differentiation of the adrenergic neurones⁷. The biochemical correlate to these morphological changes is a selective induction of tyrosine hydroxylase and dopamine β-hydroxylase⁸. We have investigated whether these effects are also reflected by changes in the preganglionic nerves.