Abstract
Direct comparison of the cell age response of 9L and KHT tumour cells derived either from tissue culture or solid tumours was achieved. Cells from dissociated KHT and 9L tumours (the latter implanted either subcutaneously or intracerebrally) and cells from tissue culture were separated into homogeneous sized populations by centrifugal elutriation. In both tumour models these homogeneous sized populations correspond to populations enriched at different stages of the cell cycle. The survival of these elutriated cell populations was measured after a single dose of Cs-137 gamma rays. For cells isolated from 9L solid tumours, there was little variation in radiosensitivity throughout the cell cycle, however, a very small but significant increase in resistance was found in late G1 cells. This lack of a large variation in radiosensitivity through the cell cycle for 9L cells from solid tumours also was seen in 9L cells growing in monolayer tissue culture. When similar experiments were performed using the KHT sarcoma tumour model, the results showed that KHT cells in vitro exhibited a fairly conventional increase in radioresistance in both mid G1 and late S. However, the cell age response of KHT cells from solid tumours was different, particularly in the late S and G2 + M phases. These data demonstrate that direct extrapolation of in vitro cell age responses to the in situ situation may not always be valid.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 24 print issues and online access
$259.00 per year
only $10.79 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Rights and permissions
About this article
Cite this article
Keng, P., Siemann, D. & Wheeler, K. Comparison of tumour age response to radiation for cells derived from tissue culture or solid tumours. Br J Cancer 50, 519–526 (1984). https://doi.org/10.1038/bjc.1984.209
Issue Date:
DOI: https://doi.org/10.1038/bjc.1984.209