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Crystal structure of SIV matrix antigen and implications for virus assembly

Abstract

SIMIAN immunodeficiency virus (SIV) is closely related to human immunodeficiency virus (HIV)1, their matrix antigens (MAs) sharing some 50% sequence identity. MA is a component of PrSSGag, the sole protein required for assembly of the virion shell2. MA targets Pr55 to the plasma membrane3, and facilitates incorporation of the virus envelope protein4 and assembly of the PrSSGag shell5. Cleavage of PrSS by the viral protease produces the mature protein of relative molecular mass 17–18K, which underlies the host-derived membrane and is important in both virus entry6 and nuclear localization of the virion core7. Here we report the crystal structure of SIV MA. The molecule forms a trimer consistent with oligomerization in vitro8, the observed virion architecture9, and various biological properties of MA.

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References

  1. Daniel, M. D. et al. Science 228, 1201–1204 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Delchambre, M. et al. EMBO J. 8, 2653–2660 (1989).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Yuan, X., Yu, X., Lee, T.-H. & Essex, M. J. Virol. 67, 6387–6394 (1993).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Freed, E. O. & Martin, M. A. J. Virol. 69, 1984–1989 (1995).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Freed, E. O., Orenstein, J. M., Buckler-White, A. J. & Martin, M. A. J. Virol. 68, 5311–5320 (1994).

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Yu, X., Yu, Q.-C., Lee, T.-H. & Essex, M. J. Virol. 66, 5667–5670 (1992).

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Bukrinsky, M. I. et al. Nature 365, 666–669 (1993).

    Article  ADS  CAS  PubMed  Google Scholar 

  8. Morikawa, Y. et al. J. Virol. 69, 4519–4523 (1995).

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Nermut, M. V., Grief, C., Hashmi, S. & Hockley, D. J. AIDS. Res. hum. Retroviruses. 9, 929–938 (1993).

    Article  CAS  PubMed  Google Scholar 

  10. Massiah, M. A. et al. J. molec. Biol. 244, 194–223 (1994).

    Article  Google Scholar 

  11. Matthews, S. et al. Nature 370, 666–668 (1994).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Levine, M., Stuart, D. & Williams, J. Acta crystallogr. A40, 600–610 (1984).

    Article  Google Scholar 

  13. Chothia, C. & Lesk, A. M. EMBO J. 5, 823–826 (1986).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Grimes, J., Basak, A. K., Roy, P. & Stuart, D. Nature 373, 167–170 (1995).

    Article  ADS  CAS  PubMed  Google Scholar 

  15. Nermut, M. V. et al. Virology 198, 288–296 (1994).

    Article  CAS  PubMed  Google Scholar 

  16. Fry, E. et al. Sem. Virology 1, 439–451 (1990).

    Google Scholar 

  17. Ehrlich, L. S., Agresta, B. E., Gelfand, C. A., Jentoft, J. & Carter, C. A. Virology 204, 515–525 (1994).

    Article  CAS  PubMed  Google Scholar 

  18. Smith, T. J. et al. Science 233, 1233–1356 (1986).

    Google Scholar 

  19. Prongay, A. J. Proc. natn. Acad. Sci. U.S.A. 87, 9980–9984 (1990).

    Article  ADS  CAS  Google Scholar 

  20. Belyaev, A. S., Stuart, D., Sutton, G. & Roy, P. J. molec. Biol. 241, 744–746 (1994).

    Article  CAS  PubMed  Google Scholar 

  21. Otwinowski, Z. in Proceedings CCP4 Study Weekend (eds. Sawyer. L., Isaacs, N. & Bailey, S.) 1-56-62 (SERC, Daresbury Laboratory, UK, 1993).

    Google Scholar 

  22. CCP4 Acta crystallogr. D50, 760–763 (1994).

  23. Jones, A. Meth. Enzym. 115, 157–171 (1985).

    Article  CAS  PubMed  Google Scholar 

  24. Brunger, A. T. X-PLOR, Version 3.1. A System for X-Ray Crystallography and NMR (Yale University Press, New Haven, CT, 1992).

    Google Scholar 

  25. Engh, A. E. & Huber, R. Acta crystallogr. A47, 392–400 (1991).

    Article  Google Scholar 

  26. Kraulis, P. J. J. appl. Crystallogr. 24, 946–950 (1991).

    Article  Google Scholar 

  27. Merritt, E. A. & Murphy, M. E. P. Acta crystallogr. D50, 869–873 (1994).

    CAS  Google Scholar 

  28. Nicholls, A., Sharp, K. A. & Honig, B. Proteins 11, 281–296 (1991).

    Article  CAS  PubMed  Google Scholar 

  29. Kabsch, W. & Sander, C. Biopolymers 22, 2577–2637 (1983).

    Article  CAS  PubMed  Google Scholar 

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Rao, Z., Belyaev, A., Fry, E. et al. Crystal structure of SIV matrix antigen and implications for virus assembly. Nature 378, 743–747 (1995). https://doi.org/10.1038/378743a0

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