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A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome

Nature volume 377, pages 309–314 (1995)Cite this article

Abstract

Interaction of the conserved CCA terminus of tRNA with rRNA in the peptidyl transferase P site has been studied by in vitro genetics. A Watson–Crick G–C pair between G2252 in a conserved hairpin loop of 23S rRNA and C74 at the acceptor end of tRNA is required for proper functional interaction of the CCA end of tRNA with the ribosomal P site. These findings establish a direct role for 23S rRNA in protein synthesis.

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References

  1. Cannon, M., Krug, R. & Gilbert, W. J. molec. Biol. 7, 360–378 (1963).

    Article  CAS  Google Scholar 

  2. Nirenberg, M. & Leder, P. Science 145, 1399–1407 (1964).

    Article  ADS  CAS  Google Scholar 

  3. Grajevskaja, R. A., Odinzov, V. B., Saminsky, E. M. & Bresler, S. E. FEBS Lett. 33, 11–14 (1973).

    Article  CAS  Google Scholar 

  4. Noller, H. F. A. Rev. Biochem. 60, 191–227 (1991).

    Article  CAS  Google Scholar 

  5. Barta, A., Steiner, G., Brosius, J., Noller, H. F. & Kuechler, E. Proc. natn. Acad. Sci. U.S.A. 81, 3607–3611 (1984).

    Article  ADS  CAS  Google Scholar 

  6. Prince, J. B., Taylor, B. H., Thurlow, D. L., Ofengand, J. & Zimmermann, R. A. Proc. natn. Acad. Sci. U.S.A. 79, 5450–5454 (1982).

    Article  ADS  CAS  Google Scholar 

  7. Moazed, D. & Noller, H. F. Cell 47, 985–994 (1986).

    Article  CAS  Google Scholar 

  8. Wower, J., Hixson, S. S. & Zimmermann, R. A. Proc. natn. Acad. Sci. U.S.A. 86, 5232–5236 (1989).

    Article  ADS  CAS  Google Scholar 

  9. Moazed, D. & Noller H. F. Cell 57, 585–597 (1989).

    Article  CAS  Google Scholar 

  10. Moazed, D. & Noller, H. F. Proc. natn. Acad. Sci. U.S.A. 88, 3725–3728 (1991).

    Article  ADS  CAS  Google Scholar 

  11. Mitchell, P., Stade, K., Osswald, M. & Brimacombe, R. Nucleic Acids Res. 21, 887–896 (1993).

    Article  CAS  Google Scholar 

  12. Döring, T., Mitchell, P., Osswald, M., Bochkariov, D. & Brimacombe, R. EMB0 J. 13, 2677–2685 (1994).

    Article  Google Scholar 

  13. von Ahsen, U. & Noller, H. F. Science 267, 234–237 (1995).

    Article  ADS  CAS  Google Scholar 

  14. Monro, R. E., Cerná, J. & Marcker, K. A. Proc. natn. Acad. Sci. U.S.A. 61, 1043–1049 (1968).

    Article  ADS  Google Scholar 

  15. Douthwaite, S., Garrett, R. A. & Wagner, R. Eur. J. Biochem. 131, 261–269 (1983).

    Article  CAS  Google Scholar 

  16. Peattie, D. & Herr, W. Proc. natn. Acad. Sci. U.S.A. 78, 2273–2277 (1981).

    Article  ADS  CAS  Google Scholar 

  17. Lieberman, K. R. & Dahlberg, A. E. J. biol. Chem. 269, 16163–16169 (1994).

    CAS  PubMed  Google Scholar 

  18. Stern, S., Moazed, D. & Noller, H. F. Meth. Enzym. 164, 481–489 (1988).

    Article  CAS  Google Scholar 

  19. Aagaard, C., Rosendahl, G., Dam, M., Powers, T. & Douthwaite, S. Biochimie 73, 1439–1444 (1991).

    Article  CAS  Google Scholar 

  20. Powers, T. & Noller, H. F. Gene 123, 75–80 (1993).

    Article  CAS  Google Scholar 

  21. Gregory, S. T., Lieberman, K. R. & Dahlberg, A. E. Nucleic Acids Res. 22, 279–284 (1994).

    Article  CAS  Google Scholar 

  22. Triman, K. et al. J. molec. Biol. 209, 645–653 (1989).

    Article  CAS  Google Scholar 

  23. Noller, H. F. FASEB J. 7, 87–96 (1993).

    Article  CAS  Google Scholar 

  24. Noller, H. F. J. Bact. 175, 5297–5300 (1993).

    Article  CAS  Google Scholar 

  25. Kaji, H., Suzuka, I. & Kaji, A. J. biol. Chem. 241, 1251–1256 (1966).

    CAS  PubMed  Google Scholar 

  26. Monro, R. E., Celma, M. L. & Vasquez, D. Nature 222, 356–358 (1969).

    Article  ADS  CAS  Google Scholar 

  27. Nierhaus, K. H. & Dohme, F. Proc. natn. Acad. Sci. U.S.A. 71, 4713–4717 (1974).

    Article  ADS  CAS  Google Scholar 

  28. Tapprich, W. E. & Hill, W. E. Proc. natn. Acad. Sci. U.S.A. 83, 556–560 (1986).

    Article  ADS  CAS  Google Scholar 

  29. Milligan, J. F., Groebe, D. R., Witherell, G. W. & Uhlenbeck, O. C. Nucleic Acids Res. 15, 8783–8798 (1987).

    Article  CAS  Google Scholar 

  30. Nierhaus, K. in Ribosomes and Protein Synthesis: A Practical Approach (ed. Spedding, G.) 161–189 (IRL, Oxford, 1990).

    Google Scholar 

  31. Noller, H. F. et al. Nucleic Acids Res. 8, 2275–2293 (1981).

    Google Scholar 

  32. Kunkel, T. A. Proc. natn. Acad. Sci. U.S.A. 82, 486–492 (1985).

    ADS  Google Scholar 

  33. Evnin, L. B. & Craik, C. S. Ann. N.Y. Acad. Sci. 542, 61–74 (1988).

    Article  ADS  CAS  Google Scholar 

  34. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  35. Powers, T. & Noller, H. F. EMBO J. 10, 2203–2214 (1991).

    Article  CAS  Google Scholar 

  36. Breitmeyer, J. B. & Noller, H. F. J. molec. Biol. 101, 297–306 (1976).

    Article  CAS  Google Scholar 

  37. Sampson, J. R., DiRenzo, A. B., Behlen, L. & Uhlenbeck, O. C. Science 243, 1363–1366 (1989).

    Article  ADS  CAS  Google Scholar 

  38. Gurevich, V. T., Pokrovskaya, I. D., Obukhova, T. A. & Zozulya, S. A. Analyt. Biochem. 195, 207–213 (1991).

    Article  CAS  Google Scholar 

  39. Noller, H. F., Hoffarth, V. & Zimniak, L. Science 256, 1416–1419 (1992).

    Article  ADS  CAS  Google Scholar 

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Authors and Affiliations

  1. Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz, California, 95064, USA

    Raymond R. Samaha, Rachel Green & Harry F. Noller

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  1. Raymond R. Samaha
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  2. Rachel Green
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  3. Harry F. Noller
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Samaha, R., Green, R. & Noller, H. A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome. Nature 377, 309–314 (1995). https://doi.org/10.1038/377309a0

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