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Cell-autonomous Fas (CD95)/Fas-ligand interaction mediates activation-induced apoptosis in T-cell hybridomas

Nature volume 373, pages 441–444 (1995)Cite this article

Abstract

A NUMBER of murine T-cell hybridomas undergo apoptosis within a few hours of activation by specific antigens, mitogens, antibodies against the T-cell antigen receptor, or a combination of phorbol ester and calcium ionophore1–3. This phenomenon has been extensively studied as a model for clonal deletion in the immune system, in which potentially autoreactive T cells eliminate themselves by apoptosis after activation, either in the thymus4 or in the periphery5. Here we show that the Fas/CD95 receptor, which can transduce a potent apoptotic signal when ligated6,7, is rapidly expressed following activation of T-cell hybridomas, as is its functional, membrane-bound ligand8. Interference with the ensuing Fas/Fas-ligand interaction inhibits activation-induced apoptosis. Because T-cell receptor ligation can induce apoptosis in a single T hybridoma cell, we suggest that the Fas/Fas-ligand interaction can induce cell death in a cell-autonomous manner.

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Authors and Affiliations

  1. Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 11149 North Torrey Pines Road, La Jolla, California, 92037, USA

    Thomas Brunner, Rona J. Mogil, Drake LaFace, Nam Jin Yoo, Artin Mahboubi, Fernando Echeverri, Seamus J. Martin & Douglas R. Green

  2. Division of Biomedical Sciences, University of California, Riverside, California, 92521, USA

    Walker R. Force & Carl F. Ware

  3. Department of Immunobiology, Immunex Research and Development Corp., Seattle, Washington, 98101, USA

    David H. Lynch

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Brunner, T., Mogil, R., LaFace, D. et al. Cell-autonomous Fas (CD95)/Fas-ligand interaction mediates activation-induced apoptosis in T-cell hybridomas. Nature 373, 441–444 (1995). https://doi.org/10.1038/373441a0

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