Abstract
UBIQUITINATION of proteins involves the concerted action of the El ubiquitin-activating enzyme, E2 ubiquitin-conjugating enzymes and E3 ubiquitin–protein 1igases1–3. It has been proposed that E3s function as 'docking proteins', specifically binding substrate proteins and specific E2s, and that ubiquitin is then transferred directly from E2s to substrates1–5. We show here that formation of a ubiquitin thioester on E6–AP, an E3 involved in the human papillomavirus E6-induced ubiquitination of p53 (refs 6–10), is an intermediate step in E6-AP-dependent ubiquitination. The order of ubiquitin transfer is from El to E2, from E2 to E6-AP, and finally from E6-AP to a substrate. This cascade of ubiquitin thioester complexes suggests that E3s have a defined enzymatic activity and do not function simply as docking proteins. The cysteine residue of E6-AP responsible for ubiquitin thioester formation was mapped to a region that is highly conserved among several proteins of unknown function, suggesting that these proteins share the ability to form thioesters with ubiquitin.
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References
Hershko, A. & Ciechanover, A. A. Rev. Biochem. 61, 761–807 (1992).
Jentsch, S. A. Rev. Genet. 26, 177–205 (1992).
Varshavsky, A. Cell 69, 725–735 (1992).
Dohmen, R. J., Madura, K., Bartel, B. & Varshavsky, A. Proc. natn. Acad. Sci. U.S.A. 88, 7351–7355 (1991).
Watkins, J. F., Sung, P., Prakash, S. & Prakash, L. Genes Dev. 7, 250–261 (1993).
Scheffner, M., Werness, B. A., Huibregtse, J. M., Levine, A. J. & Howley, P. M. Cell 63, 1129–1136 (1990).
Huibregtse, J. M., Scheffner, M. & Howley, P. M. EMBO J. 13, 4129–4135 (1991).
Huibregtse, J. M., Scheffner, M. & Howley, P. M. Molec. cell. Biol. 13, 775–784 (1993).
Huibregtse, J. M., Scheffner, M. & Howley, P. M. Molec. cell. Biol. 13, 4918–4927 (1993).
Scheffner, M., Huibregtse, J. M., Vierstra, R. D. & Howley, P. M. Cell 75, 495–505 (1993).
Girod, P.-A., Carpenter, T. P., van Nocker, S., Sullivan, M. L. & Vierstra, R. D. PI. J. 3, 545–552 (1993).
Scheffner, M., Huibregtse, J. M. & Howley, P. M. Proc. natn. Acad. Sci. U.S.A. 91, 8797–8801 (1994).
Haas, A. L., Warms, J. V. B., Hershko, A. & Rose, I. A. J. biol. Chem. 257, 2543–2548 (1982).
Ciechanover, A., Elias, S., Heller, H. & Hershko, A. J. biol. Chem. 257, 2537–2542 (1982).
Hatfield, P. M. & Vierstra, R. D. J. biol. Chem. 267, 14799–14803 (1992).
Pickart, C. M. & Rose, I. A. J. biol. Chem. 260, 1573–1581 (1985).
Haas, A. L. & Bright, P. M. J. biol. Chem. 263, 13258–13267 (1988).
Berleth, E. S. et al. J. biol. Chem. 267, 16403–16411 (1992).
Bartel, B., Wünning, I. & Varshavsky, A. EMBO J. 9, 3179–3189 (1990).
Sung, P., Prakash, S. & Prakash, L. J. molec. Biol. 221, 745–749 (1991).
Müller, D., Rehbein, M., Baumeister, H. & Richter, D. Nucleic Acids Res. 20, 1471–1475 (1992).
Pascolo, S. et al. Yeast 8, 987–995 (1992).
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Scheffner, M., Nuber, U. & Huibregtse, J. Protein ubiquitination involving an E1–E2–E3 enzyme ubiquitin thioester cascade. Nature 373, 81–83 (1995). https://doi.org/10.1038/373081a0
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DOI: https://doi.org/10.1038/373081a0
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