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Cloning and characterization of the vasopressin-regulated urea transporter

Nature volume 365, pages 844–847 (1993)Cite this article

Abstract

UREA is the principal end product of nitrogen metabolism in mammals1. Movement of urea across cell membranes was originally thought to occur by lipid-phase permeation, but recent studies have revealed the existence of specialized transporters with a low affinity for urea (Km > 200 mM)2. Here we report the isolation of a complementary DNA from rabbit renal medulla that encodes a 397-amino-acid membrane glycoprotein, UT2, with the functional characteristics of the vasopressin-sensitive urea transporter previously described in in vitro-perfused inner medullary collecting ducts3,4. UT2 is not homologous to any known protein and displays a unique pattern of hydrophobicity. Because of the central role of this transporter in fluid balance1,3–7 and nitrogen metabolism8, the study of this protein will provide important insights into the urinary concentrating mechanism and nitrogen balance.

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Authors and Affiliations

  1. Renal Division, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School,

    Guofeng You, Craig P. Smith, Yoshikatsu Kanai, Wen-Sen Lee, Matthias Stelzner & Matthias A. Hediger

  2. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 75 Francis Street, Boston, Massachusetts, 02115, USA

    Guofeng You, Craig P. Smith, Yoshikatsu Kanai, Wen-Sen Lee, Matthias Stelzner & Matthias A. Hediger

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  1. Guofeng You
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You, G., Smith, C., Kanai, Y. et al. Cloning and characterization of the vasopressin-regulated urea transporter. Nature 365, 844–847 (1993). https://doi.org/10.1038/365844a0

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