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Largest subunit of Drosophila transcription factor IID directs assembly of a complex containing TBP and a coactivator

Nature volume 362, pages 511–517 (1993)Cite this article

Abstract

The TFIID complex consists of the TATA-binding protein (TBP) and associated factors (TAFs) serving to mediate transcriptional activation by promoter-specific regulators. Here we report the cloning of Drosophila TAFII250 and the assembly of a partial complex containing recombinant TBP, TAFII110 and the C-terminal domain of TAFII250. This triple complex supports Sp1 activation and reveals specific interactions between TAFII250, TBP and TAFII110.

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Author notes

  1. Brian David Dynlacht

    Present address: Massachusetts General Hospital, Cancer Center, Charlestown, Massachusetts, 02129, USA

Authors and Affiliations

  1. Howard Hughes Medical Institute, Department of Molecular and Cell Biology, 401 Barker Hall, University of California at Berkeley, Berkeley, California, 94720, USA

    Robert O. J. Weinzierl, Brian David Dynlacht & Robert Tjian

Authors
  1. Robert O. J. Weinzierl
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  2. Brian David Dynlacht
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  3. Robert Tjian
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Weinzierl, R., Dynlacht, B. & Tjian, R. Largest subunit of Drosophila transcription factor IID directs assembly of a complex containing TBP and a coactivator. Nature 362, 511–517 (1993). https://doi.org/10.1038/362511a0

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