Abstract
IN the limb bud of the 5-day-old avian embryo, when primary muscle fibre formation is beginning and before specific muscles appear, differences in the expression of fast and slow myosin heavy chain genes can be detected among primary fibres of the premuscle masses1,2. Myoblasts that form colonies of fibres of specific types can be isolated from these limb buds3. To assess the role of myoblast commitment in specifying fibre types during embryonic development, we cloned myoblasts of specific types from embryonic and adult muscles, transfected them with a reporter gene, and transferred them into developing limb buds. After transfer, cloned myoblastsF formed fibres in the limb with the same patterns of myosin heavy chain gene expression as the fibres they formed in cell culture. These results demonstrate that initial skeletal muscle fibre type diversity during avian limb development can originate, in part, from the commitment of distinct myoblast types to the formation of specific fibre types.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Crow, M. T. & Stockdale, F.E. Devl Biol. 113, 238–254 (1986).
Page, S. et al. Devl Biol. 154, 118–128 (1992).
Miller, J. B. & Stockdale, F. E. J. Cell Biol. 103, 2197–2208 (1986).
Butler, J., Cosmos, E. & Cauwenbergs, P. Development 102, 763–772 (1988).
McLennan, I. S. Devl Biol. 98, 287–294 (1983).
Miller, J. B. & Stockdale, F. E. Proc. natn. Acad. Sci. U.S.A. 83, 3860–3864 (1986)
Stockdale, F. E. & Miller, J. B. Devl Biol. 123, 1–9 (1987).
Stockdale, F. E. Devl Biol. 154, 284–298 (1992).
Miller, J. B. & Stockdale, F. E. Devl Biol. 136, 393–404 (1989).
Ordahl, C. P., Mar, J., Clyne, J. & Simpson, P. Molecular Biology of Muscle Development. UCLA Symp. Molec. cell. Biol. Vol. 29 547–558 (Liss, New York, 1986).
Feldman, J. L. & Stockdale, F. E. Devl Biol. 143, 320–334 (1990).
Pette, D. & Vrbová, G. Muscle Nerve 8, 676–689 (1985).
Harris, A. J., Fitzsimons, R. B. & McEwan, J. C. Development 107, 751–769 (1989).
Gauthier, G. F., Ono, R. D. & Hobbs, A. W. Devl Biol. 105, 144–154 (1984).
Everett, A. W. et al. Biochemistry 23, 1596–1599 (1984).
Izumo, S., Nadal-Ginard, B. & Mahdavi, V. Science 231, 597–600 (1986).
Hughes, S. M. & Blau, H. M. Cell 68, 659–671 (1992).
Sofer, W. & Ursprung, H. J. biol. Chem 243, 3110–3115 (1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
DiMario, J., Fernyak, S. & Stockdale, F. Myoblasts transferred to the limbs of embryos are committed to specific fibre fates. Nature 362, 165–167 (1993). https://doi.org/10.1038/362165a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/362165a0
This article is cited by
-
Control of satellite cell function in muscle regeneration and its disruption in ageing
Nature Reviews Molecular Cell Biology (2022)
-
Lineage-based primary muscle fiber type diversification independent of MEF2 and NFAT in chick embryos
Journal of Muscle Research and Cell Motility (2011)
-
The ups and downs of gene regulation by electrical activity in skeletal muscles
Journal of Muscle Research and Cell Motility (2009)
-
Controlled differentiation of myoblast cells into fast and slow muscle fibers
Cell and Tissue Research (2008)
-
Hedgehog can drive terminal differentiation of amniote slow skeletal muscle
BMC Developmental Biology (2004)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
