Abstract
IN Snell (dw) and Jackson (dwj) dwarf mice, mutations in the gene encoding Pit-1, a tissue-specific POU-domain transcription factor, lead to the absence of somatotroph, lactotroph and thyrotroph cells1–6. Pre-somatotroph proliferation is stimulated by increased intracellular levels of cyclic AMP, normally induced by growth hormone releasing factor (GRF; refs 7–17). Here we report the cloning of mouse and rat complementary DNAs encoding a new member of the seven-transmembrane-helix, G-protein-coupled receptor family restricted to the pituitary gland, which mediates increases in intracellular cAMP and cAMP-dependent gene transcription in response to GRF. The receptor is expressed in a spatial and temporal pattern corresponding precisely to growth hormone gene expression, and neither is expressed in dw/dw mice. The pituitary hypoplasia in these mice thus appears to be due, at least in part, to the absence of GRF receptor, which is in turn due to the absence of functional Pit-1.
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References
Li, S. et al. Nature 347, 528–530 (1990).
Ingraham, H. A. et al. Cell 55, 519–529 (1988).
Simmons, D. M. et al. Genes Dev. 4, 695–711 (1990)
Wilson, D. B. & Wyatt, D. P. Anal Embryol 174, 277–282 (1986).
Rouse, M., Bartke, A., Dumont, F. & Dubois, M. P. Cell Tissue Res. 223, 415–420 (1982)
Yashiro, T. Arai, M., Miyashita, E., Yamashita, K. & Suzuki, T. Cell Tissue Res. 251, 249–255 (1988)
Landis, C. A. et al. Nature 340, 692–696 (1989).
Rivier, J. Spiess, J., Thorner, M. & Vale, W. Nature 300, 276–278 (1982).
Guillemin, R. et al. Science 218, 585–587 (1982).
Thorner, M. O. et al. J. clin. Invest. 70, 965–977 (1982).
Billestrup, N., Swanson, L. W. & Vale, W. Proc. natn. Acad Sci. U.S.A. 83, 6854–6857 (1986)
Burton, F. H., Hasel, K. W., Bloom, F. E. & Sutcliffe, J. G. Nature 350, 74–77 (1991).
Seifert, H., Perrin, M., Rivier, J. & Vale, W. Nature 313, 487–489 (1985).
Bilezikjian, L. & Vale, W. Endocrinology 113, 1726–1731 (1983).
Gick, G. G. et al. Proc. natn. Acad. Sci. U.S.A. 81, 1553–1555 (1984).
Mayo, K. E. et al. Molec. Endocr. 606–612 (1988).
Struthers, R. S., Vale, W. W., Arias, C., Sawchenko, P. E. & Montminy, M. R. Nature 350, 622–624 (1991).
Collins, S., Caron, M. G. & Lefkowitz, R. J. Trends biochem. sa. 17, 37–39 (1992).
Ishihari, T., Nakamura, S., Kazira, Y., Takahashi, T. & Nagata, S. EMB0 J. 10, 1635–1641 (1991).
Jüppner, H. et al. Science 1022–1025 (1991)
Lin, H. Y. et al. Science 254, 1022–1024 (1991).
Ishihara, T., Sigemoto, R. Mori, K., Takahashi, K. & Nagata, S. Neuron 8, 815–819 (1992).
Montminy, M. R., Sevarino, K. A., Wagner, J. A., Mandel, G. & Goodman, R. H. Proc. natn. Acad. Sci. U.S.A. 83, 6682–6686 (1986).
Neutsch, P. J., Jameson, J. L. & Habener, J. F. J. biol. Chem. 262, 12169–12171 (1987).
Gonzalez, G. A. & Montminy, M. R. Cell 59, 675–680 (1989).
Gonzalez, G. A. et al. Nature 337, 749–752 (1989).
Bodner, M. et al. Cell 55, 505–518 (1988).
Fox, S. R. et al. Molec. Endocr. 4, 1069–1080 (1990).
Ishikawa, K., Katakami, H., Jansson, J.-O. & Frohman, L. A. Neuroendocrinology 43, 537–542 (1986)
Baird, A., Wehrenberg, W. & Ling, N. Regul. Pep. 10, 23–28 (1984).
Eicher, E. M. & Beamer, W. G. J. Hered 67, 87–91 (1976).
Frohman, L. A. & Jansson, J.-O. Endocr. Rev. 7, 236–239 (1986).
Sanger, F., Micklen, J. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5466 (1979).
Brown, B. L. Albano, J. D., Ekins, R. P. & Sgherzi, A. M. Biochem. J. 171, 561–562 (1971).
Mayo, K. Molec. Endocr. 6, 1734–1744 (1992).
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Lin, C., Lin, SC., Chang, CP. et al. Pit-1-dependent expression of the receptor for growth hormone releasing factor mediates pituitary cell growth. Nature 360, 765–768 (1992). https://doi.org/10.1038/360765a0
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DOI: https://doi.org/10.1038/360765a0
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