Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Zebrafish pax[b] is involved in the formation of the midbrain–hindbrain boundary

Abstract

AMONG the genes thought to be involved in patterning the nervous system are a family of developmentally regulated paired box-containing (Pax) genes. Mutations in some of these Pax genes lead to severe developmental abnormalities. Zebrafish pax[b] (pax[zf-b]) is a member of the Pax gene family that is expressed in the presumptive posterior midbrain from the end of gastrulation and, at later stages, in other localized regions of the developing embryo1. Here we show that injection of antibodies raised against the pax[b] protein causes a localized malformation at the mid-brain–hindbrain boundary. In situ hybridizations demonstrate that antibody injection causes downregulation of pax[b] transcripts in the posterior midbrain and alteration of wnt-1 and eng-2 expression in this area. The data demonstrate an involvement of pax[b] in the formation of the midbrain–hindbrain junction.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Krauss, S., Johansen, T., Korzh, V. & Fjose, A. Development 113, 1193–1206 (1991).

    CAS  PubMed  Google Scholar 

  2. Deutsch, U., Dressier, G. R. & Gruss, P. Cell 53, 617–625 (1988).

    Article  CAS  Google Scholar 

  3. Dressier, G. R., Deutsch, U., Chowdhury, K., Nornes, H. O. & Gruss, P. Development 109, 787–795 (1990).

    Google Scholar 

  4. Plachov, D. et al. Development 110, 643–651 (1990).

    CAS  Google Scholar 

  5. Goulding, M. D., Chalepkis, G., Deutsch, U., Erselius, J. R., & Gruss, P. EMBO J. 10, 1135–1147 (1991).

    Article  CAS  Google Scholar 

  6. Jostes, B., Walther, C. & Gruss, P. Mech. Dev. 33, 27–38 (1991).

    Article  Google Scholar 

  7. Krauss, S. et al. EMBO J. 10, 3609–3619 (1991).

    Article  CAS  Google Scholar 

  8. Walther, C. & Gruss, P. Development 113, 1435–1449 (1991).

    CAS  PubMed  Google Scholar 

  9. Walther, C. et al. Genomics 11, 424–434 (1991).

    Article  CAS  Google Scholar 

  10. Chalepakis, G. et al. Cell 66, 873–884 (1991).

    Article  CAS  Google Scholar 

  11. Treisman, J., Harris, E. & Desplan, C. Genes Dev. 5, 594–604 (1991).

    Article  CAS  Google Scholar 

  12. Hill, R. E. et al. Nature 354, 522–525 (1991).

    Article  ADS  CAS  Google Scholar 

  13. Ton, C. C. T. et al. Cell 67, 1059–1074 (1991).

    Article  CAS  Google Scholar 

  14. Baldwin, C. T., Hoth, C. F., Amos, J. A., da-Silva, E. O. & Milunsky, A. Nature 355, 637–638 (1992).

    Article  ADS  CAS  Google Scholar 

  15. Tassabehji, M. et al. Nature 355, 635–636 (1992).

    Article  ADS  CAS  Google Scholar 

  16. Mikkola, J. et al. J. Neurobiol. 23, 933–946 (1992).

    Article  CAS  Google Scholar 

  17. Epstein, D. J., Vekemans, M. & Gros, P. Cell 67, 767–774 (1991).

    Article  CAS  Google Scholar 

  18. McMahon, A. P. & Bradley, A. Cell 62, 1073–1085 (1990).

    Article  CAS  Google Scholar 

  19. Thomas, K. R. & Capecchi, M. R. Nature 346, 847–850 (1990).

    Article  ADS  CAS  Google Scholar 

  20. Joyner, A. L., Herrup, K., Auerbach, B. A., Davis, C. A. & Rossant, J. Science 251, 1239–1243 (1991).

    Article  ADS  CAS  Google Scholar 

  21. Thomas, K. R., Musci, T. S., Neumann, P. E. & Capecchi, M. Cell 67, 969–976 (1991).

    Article  CAS  Google Scholar 

  22. McMahon, A. P., Joyner, A. L., Bradley, A. & McMahon, J. A. Cell 69, 581–595 (1992).

    Article  CAS  Google Scholar 

  23. Nüsse, R. & Varmus, H. E. Cell 31, 99–109 (1982).

    Article  Google Scholar 

  24. Papkoff, J., Brown, A. M. C. & Varmus, H. E. Molec. cell Biol. 7, 3978–3984 (1987).

    Article  CAS  Google Scholar 

  25. Molven, A., Njølstad, P. R. & Fjose, A. EMBO J. 10, 799–807 (1991).

    Article  CAS  Google Scholar 

  26. Joyner, A. L., Kornberg, T., Coleman, K. G., Cox, D. R. & Martin, G. R. Cell 43, 29–37 (1985).

    Article  CAS  Google Scholar 

  27. Hiromi, Y. & Gehring, W. J. Cell 50, 963–974 (1987).

    Article  CAS  Google Scholar 

  28. DiNardo, S. & O'Farrell, P. H. Genes Dev. 1, 1212–1225 (1987).

    Article  CAS  Google Scholar 

  29. Ingham, P. W., Baker, N. E. & Martinez-Arias, A. Nature 331, 73–75 (1988).

    Article  ADS  CAS  Google Scholar 

  30. Hidalgo, A. & Ingham, P. Development 110, 291–301 (1990).

    CAS  Google Scholar 

  31. Bopp, D., Burri, M., Baumgartner, S., Frigerio, G. & Noll, M. Cell 47, 1033–1049 (1986).

    Article  CAS  Google Scholar 

  32. Grunwald, D. J., Kimmel, C. B., Westerfield, M., Walker, C. & Streisinger, G. Devl Biol. 126, 115–128 (1988).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Krauss, S., Maden, M., Holder, N. et al. Zebrafish pax[b] is involved in the formation of the midbrain–hindbrain boundary. Nature 360, 87–89 (1992). https://doi.org/10.1038/360087a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/360087a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing