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Mutation of protein kinase A causes heterochronic development of Dictyostelium

Abstract

IN heterochronic mutants the relative timing of developmental events is altered compared with the wild type. This generally results in a disordered embryo1,2, though heterochronic mutations may also be an important source of evolutionary variation3. In the rapidly developing (rde) mutants of Dictyostelium, stalk and spore cells differentiate before morphogenesis is complete. We have traced the lesion in one class of these mutants to the regulatory subunit of cyclic AMP-dependent protein kinase (pk-A). Inactivation of this protein results in the unrestrained activity of the catalytic subunit, so prematurely triggering terminal cell differentiation.

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References

  1. Ambros, V. & Horvitz, H. R. Science 226, 409–416 (1984).

    Article  ADS  CAS  Google Scholar 

  2. Ruvkun, G. & Giusto, J. Nature 338, 313–319 (1989).

    Article  ADS  CAS  Google Scholar 

  3. Bonner, J. T. Am. Nat. 119, 530–552 (1982).

    Article  Google Scholar 

  4. Sonneborn, D., White, G. & Sussman, M. Devl Biol. 7, 79–93 (1963).

    Article  Google Scholar 

  5. Abe, K. & Yanagisawa, K. Devl Biol. 95, 200–210 (1983).

    Article  CAS  Google Scholar 

  6. Kessin, R. H. Cell 10, 703–708 (1977).

    Article  CAS  Google Scholar 

  7. Kay, R. R. Development 105, 753–759 (1989).

    CAS  Google Scholar 

  8. de Gunzburg, J., Part, D., Guiso, N. & Veron, M. Biochemistry 23, 3805–3812 (1984).

    Article  CAS  Google Scholar 

  9. de Gunzburg, J. & Veron, M. EMBO J. 1, 1063–1068 (1982).

    Article  Google Scholar 

  10. Town, C. D., Gross, J. D. & Kay, R. R. Nature 262, 717–719 (1976).

    Article  ADS  CAS  Google Scholar 

  11. Mutzel, R., Lacombe, M. L., Simon, M. N., De Gunzburg, J. & Veron, M. Proc. natn. Acad. Sci. U.S.A. 84, 6–10 (1987).

    Article  ADS  CAS  Google Scholar 

  12. Taylor, S. S., Buechler, J. A. & Yonemoto, W. A. Rev. Biochem. 59, 971–1005 (1990).

    Article  CAS  Google Scholar 

  13. Knighton, D. R. et al. Science 253, 414–420 (1991).

    Article  ADS  CAS  Google Scholar 

  14. Rangel-Aldao, R. & Rosen, O. M. J. biol. Chem. 251, 3375–3380 (1976).

    CAS  PubMed  Google Scholar 

  15. Kuret, J., Johson, K. E., Nicolette, C. & Zoller, M. J. J. biol. Chem. 251, 9149–9154 (1988).

    Google Scholar 

  16. Simon, M. N. et al. EMBO J. 8, 2039–2043 (1989).

    Article  CAS  Google Scholar 

  17. Merkle, R. K., Cooper, K. K. & Rutherford, C. L. Cell Differ. 14, 257–266 (1984).

    Article  CAS  Google Scholar 

  18. Newell, P. C., Europe-Finner, G. N., Small, N. V. & Liu, G. J. Cell Sci. 89, 123–127 (1988).

    CAS  PubMed  Google Scholar 

  19. Firtel, R. A., van Haastert, P. J. M., Kimmel, A. R. & Devreotes, P. N. Cell 58, 235–239 (1989).

    Article  CAS  Google Scholar 

  20. Devreotes, P. N. & Steck, T. L. J. Cell Biol. 80, 300–309 (1979).

    Article  CAS  Google Scholar 

  21. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  22. Titani, K. et al. Biochemistry 23, 4193–4199 (1984).

    Article  CAS  Google Scholar 

  23. Takio, K., Smith, S. B., Krebs, E. G., Walsh, K. A. & Titani, K. Biochemistry 23, 4200–4206 (1984).

    Article  CAS  Google Scholar 

  24. Innis, M. A., Gelfand, D. H., Sninsky, J. J. & White, T. J. PCR Protocols (Academic Press, London, 1990).

    Google Scholar 

  25. Reymond, C. D. Nucleic Acids Res. 15, 8118 (1987).

    Article  CAS  Google Scholar 

  26. Mutzel, R., Simon, M. N., Lacombe, M. L. & Veron, M. Biochemistry 27, 481–486 (1988).

    Article  CAS  Google Scholar 

  27. Scott, J. D., Glaccum, M. B., Fischer, E. H. & Krebs, E. G. Proc. natn. Acad. Sci. U.S.A. 83, 1613–1616 (1986).

    Article  ADS  CAS  Google Scholar 

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Simon, MN., Pelegrini, O., Veron, M. et al. Mutation of protein kinase A causes heterochronic development of Dictyostelium. Nature 356, 171–172 (1992). https://doi.org/10.1038/356171a0

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