Abstract
IN mammalian cells, short peptides derived from intracellular proteins are displayed on the cell membrane associated with class I molecules of the major histocompatibility complex (MHC). The surface presentation of class I-peptide complexes presumably alerts the immune system to intracellular viral protein synthesis. Peptides derived from the cytosol must reach the cisternae of the endoplasmic reticulum where they are required for the assembly of stable class I molecules1–11, and it has been proposed that the products of the two MHC-encoded ATP-binding cassette (ABC) transporter genes12–15 function to deliver the peptides across the membrane of the endoplasmic reticulum. This idea is supported by experiments in which transfection of a human cell line defective in class I expression with a complementary DNA of one of these genes restored cell surface expression levels16. Here we show that the complete phenotype of the mouse mutant cell line RMA-S, in which lack of surface expression of stable class I molecules correlates with an inability to present viral peptides originating in the cytosol6–10,17, is repaired by the cDNA of the other transporter gene. These results are consistent with the possibility that the two transporter polypeptides form a heterodimer.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Townsend, A. R. M., Gotch, F. M. & Davey, J. Cell 42, 457–467 (1985).
Yewdell, J. W. & Bennink, J. R. Science 244, 1072–1075 (1989).
Nuchtern, J. G., Bonifacino, J. S., Biddison, W. E. & Klausner, R. D. Nature 339, 223–226 (1989).
Hsu, V. W. et al. Nature 352, 441–444 (1991).
Sweetser, M. T., Morrison, L. A., Braciale, V. L. & Braciale, T. J. Nature 342, 180–182 (1989).
Townsend, A., Elliott, T., Cerundolo, V., Foster, L., Barber, B. & Tse, A. Cell 62, 285–295 (1990).
Elliott, T., Cerundolo, V., Elvin, J. & Townsend, A. Nature 351, 402–406 (1991).
Ljunggren, H-G. et al. Nature 346, 476–480 (1990).
Schumacher, T. N. M. et al. Cell 62, 563–567 (1990).
Schumacher, T. N. M. et al. Nature 350, 703–706 (1991).
Silver, M. L., Parker, K. C. & Wiley, D. C. Nature 350, 619–622 (1991).
Monaco, J. J., Cho, S. & Attaya, M. Science 250, 1723–1726 (1990).
Deverson, E. V. et al. Nature 348, 738–741 (1990).
Trowsdale, J. et al. Nature 348, 741–744 (1990).
Spies, T. et al. Nature 348, 744–747 (1990).
Spies, T. & DeMars, R. Nature 351, 323–324 (1991).
Townsend, A. et al. Nature 340, 443–448 (1989).
Kärre, K., Ljunggren, H-G., Piontek, G. & Kiessling, R. Nature 319, 675–678 (1986).
Drake, J. W. & Baltz, R. H. A. Rev. Biochem. 45, 11–37 (1976).
Ljunggren, H-G. et al. J. Immun. 142, 2911–2917 (1989).
Townsend, A. et al. Cell 44, 959–968 (1986).
Parham, P. Nature 351, 271–272 (1991).
Eisenberg, D. A. Rev. Biochem. 53, 595–623 (1984).
Powis, S. J., Howard, J. C. & Butcher, G. W. J. exp. Med. 173, 913–921 (1991).
Hämmerling, G. J. et al. Proc. natn. Acad. Sci. U.S.A. 79, 4737–4741 (1982).
Gunning, P. et al. Proc. natn. Acad. Sci. U.S.A. 84, 4831–4835 (1987).
Koch, S., Koch, N., Robinson, P. & Hämmerling, G. Transplantation 36, 177–180 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Powis, S., Townsend, A., Deverson, E. et al. Restoration of antigen presentation to the mutant cell line RMA-S by an MHC-linked transporter. Nature 354, 528–531 (1991). https://doi.org/10.1038/354528a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/354528a0
This article is cited by
-
A survey of the adaptive immune genes of the polka-dot batfish Ogcocephalus cubifrons
BMC Immunology (2023)
-
ALK peptide vaccination restores the immunogenicity of ALK-rearranged non-small cell lung cancer
Nature Cancer (2023)
-
A personal retrospective on the mechanisms of antigen processing
Immunogenetics (2019)
-
A highly conserved sequence of the viral TAP inhibitor ICP47 is required for freezing of the peptide transport cycle
Scientific Reports (2017)
-
Introduction of a point mutation into an HLA class I single-chain trimer induces enhancement of CTL priming and antitumor immunity
Molecular Therapy - Methods & Clinical Development (2014)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
