Abstract
Here we evaluate the idea that the Golgi is in dynamic equilibrium with the endoplasmic reticulum (ER). In cytoplasts that lack the Golgi apparatus, no regrowth of the Golgi is observed, nor is any transport from the ER to the cell surface detected. However, introduction of the smallest measurable amount of Golgi (equivalent to a few per cent per cell) yields significant exocytic transport. Our results indicate that the steady-state levels of Golgi in the ER are far smaller than the 30% that has been postulated, and that the Golgi may be an independent organelle and not simply an extension of the ER.
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Acknowledgements
We thank J. Shorter, M. Lowe, A. Price, H. Meyer, J. Seemann and D. Sheff for discussion and reading of the manuscript. We also thank D. Shima, M. Lowe, D. Mundy, H-P. Hauri, F. Gorelick, F. Watt, G. M. Waters and T. Suganuma for antibodies, and A. Maniotis for advice on microsurgery. We are grateful to M. Kabaservice for help with preparation of the manuscript. This work was supported by NIH grant no. GM60478-01 (to G.W.).
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Pelletier, L., Jokitalo, E. & Warren, G. The effect of Golgi depletion on exocytic transport. Nat Cell Biol 2, 840–846 (2000). https://doi.org/10.1038/35041089
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DOI: https://doi.org/10.1038/35041089
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