Abstract
In plants there are three microtubule arrays involved in cellular morphogenesis that have no equivalent in animal cells. In animals, microtubules are decorated by another class of proteins – the structural MAPS – which serve to stabilize microtubules and assist in their organization. The best-studied members of this class in plants are the MAP-65 proteins that can be purified together with plant microtubules after several cycles of polymerization and depolymerization. Here we identify three similar MAP-65 complementary DNAs representing a small gene family named NtMAP65-1, which encode a new set of proteins, collectively called NtMAP65-1. We show that NtMAP65-1 protein localizes to areas of overlapping microtubules, indicating that it may function in the behaviour of antiparallel microtubules in the mitotic spindle and the cytokinetic phragmoplast.
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Acknowledgements
This work was supported by grants from the Biotechnology and Biological Sciences Research Council and from Bayer AG.
Correspondence and requests for materials should be addressed to P.J.H The amino-acid sequences of NtMAP65–1a, NtMAP65–1b and NtMAP65–1c have been deposited at GenBank under accession nos AJ289862, AJ289863 and AJ289864, respectively.
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Correspondence and requests for materials should be addressed to P. J. H. The amino-acid sequences of NtMAP65-1a, NtMAP65-1b and NtMAP65-1c have been deposited at GenBank under accession nos AJ289862, AJ289863 and AJ289864, respectively.
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Smertenko, A., Saleh, N., Igarashi, H. et al. A new class of microtubule-associated proteins in plants. Nat Cell Biol 2, 750–753 (2000). https://doi.org/10.1038/35036390
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DOI: https://doi.org/10.1038/35036390
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