Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Interaction at a distance between λrepressers disrupts gene activation

Nature volume 336, pages 353–357 (1988)Cite this article

Abstract

The λ represser is an activator as well as a represser of transcription. The activation function is blocked by interaction with another λrepresser molecule bound upstream on the same DNA molecule. This example of negative control at a distance involves formation of a DNA loop

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Ptashne, M. Nature 332, 697–701 (1986).

    Article  ADS  Google Scholar 

  2. Hochschild, A. & Ptashne, M. Cell 44, 925–933 (1986).

    Article  CAS  Google Scholar 

  3. Griffith, J., Hochschild, A. & Ptashne, M. Nature 322, 750–752 (1986).

    Article  ADS  CAS  Google Scholar 

  4. Johnson, A. D., Meyer, B. J. & Ptashne, M. Proc. natn. Acad. Sci. U.S.A. 76, 5061–5065 (1979).

    Article  ADS  CAS  Google Scholar 

  5. Irani, M. H., Orosz, L. & Adhya, S. Cell 32, 783–788 (1983).

    Article  CAS  Google Scholar 

  6. Kuhnke, G. et al. EMBO J. 5, 167–173 (1986).

    Article  CAS  Google Scholar 

  7. Dandanell, G. & Hammer, K. EMBO J. 4, 3333–3338 (1985).

    Article  CAS  Google Scholar 

  8. Valentin-Hansen, P., Albrechtsen, B. & Love Larsen, J. E. EMBO J. 5, 2015–2021 (1986).

    Article  CAS  Google Scholar 

  9. Eismann, E., v. Wilcken-Bergmann, B. & Muller-Hill, B. J. molec. Biol. 195, 949–952 (1987).

    Article  CAS  Google Scholar 

  10. Borowiec, J. A., Zhang, L., Sasse-Dwight, S. & Gralla, J. J. molec. Biol. 196, 101–111 (1987).

    Article  CAS  Google Scholar 

  11. Sasse-Dwight, S. & Gralla, J. D. J. molec. Biol. (in the press).

  12. Dunn, T. M., Hahn, S., Ogden, S. & Schleif, R. F. Proc. natn. Acad. Sci. U.S.A. 81, 5017–5020 (1984).

    Article  ADS  CAS  Google Scholar 

  13. Martin, K., Huo, L. & Schleif, R. Proc. natn. Acad. Sci. U.S.A. 83, 3654–3658 (1986).

    Article  ADS  CAS  Google Scholar 

  14. Johnson, A. D. et al. Nature 294, 217–223 (1981).

    Article  ADS  CAS  Google Scholar 

  15. Ptashne, M. A Genetic Switch (Cell Press, Palo Alto and Blackwell Publishing, 1986).

    Google Scholar 

  16. Meyer, B. J., Maurer, R. & Ptashne, M. J. Molec. Biol. 139, 163–194 (1980).

    Article  CAS  Google Scholar 

  17. Guarente, L., Nye, J. S., Hochschild, A. & Ptashne, M. Proc. natn. Acad. Sci. U.S.A. 79, 2236–2239 (1982).

    Article  ADS  CAS  Google Scholar 

  18. Hochschild, A., Irwin, N. & Ptashne, M. Cell 32, 319–325 (1983).

    Article  CAS  Google Scholar 

  19. Bushman, F. D. & Ptashne, M. Cell 54, 191–197 (1988).

    Article  CAS  Google Scholar 

  20. Chadwick, P., Pirotta, V., Steinberg, R., Hopkins, N. & Ptashne, M. Cold Spring Harbor Symp. quant. Biol. 35, 283–294 (1970).

    Article  CAS  Google Scholar 

  21. Lee, N., Francklyn, C. & Hamilton, R. P. Proc. natn. Acad. Sci. U.S.A. 84, 8814–8818 (1987).

    Article  ADS  CAS  Google Scholar 

  22. Hamilton, E. P. & Lee, N. Proc. natn. Acad. Sci. U.S.A. 85, 1749–1753 (1988).

    Article  ADS  CAS  Google Scholar 

  23. Brand, A. H., Micklem, G. & Nasmyth, K. Cell 51, 709–719 (1987).

    Article  CAS  Google Scholar 

  24. Shore, D. & Nasmyth, K. Cell 51, 721–732 (1987).

    Article  CAS  Google Scholar 

  25. Buchman, A. R., Kimmerly, W. J., Rine, J. & Kornberg, R. D. Molec. cell. Biol. 8, 210–225 (1988).

    Article  CAS  Google Scholar 

  26. Backman, K. & Ptashne, M. Cell 13, 65–71 (1978).

    Article  CAS  Google Scholar 

  27. Pabo, C. O., Sauer, R. T., Surtevant, J. M. & Ptashne M. Proc. natn. Acad. Sci. U.S.A. 76, 1608–1612 (1979).

    Article  ADS  CAS  Google Scholar 

  28. Sauer, R. T., Pabo, C. O., Meyer, B. J. Ptashne, M. & Backman, K. C. Nature 279, 396–400 (1979).

    Article  ADS  CAS  Google Scholar 

  29. Pabo, C. O. & Lewis, M. Nature 298, 443–447 (1982).

    Article  ADS  CAS  Google Scholar 

  30. Dente, L., Cesareni, G. & Cortese, R. Nucleic Acids Res. 11, 1645–1655 (1983).

    Article  CAS  Google Scholar 

  31. Wharton, R. P. & Ptashne, M. Nature 326, 888–891 (1987).

    Article  ADS  CAS  Google Scholar 

  32. Yu, X.-M. & Reznikoff, W. S. Nucleic Acids Res. 12, 5449–5464 (1984).

    Article  CAS  Google Scholar 

  33. Johnson, A. D., Pabo, C. O. & Sauer, R. T. Meth. Enzym. 65, 839–856 (1980).

    Article  CAS  Google Scholar 

  34. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–540 (1980).

    Article  CAS  Google Scholar 

  35. Amann, E., Brosius, J. & Ptashne, M. Gene 25, 167–178 (1983).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts, 02138, USA

    A. Hochschild & M. Ptashne

Authors
  1. A. Hochschild
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Ptashne
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hochschild, A., Ptashne, M. Interaction at a distance between λrepressers disrupts gene activation. Nature 336, 353–357 (1988). https://doi.org/10.1038/336353a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/336353a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing