Abstract
Agrobacterium tumefaciens induces tumorous proliferations on dicotyledonous plants by transferring a specific region (T-DNA) of its tumour-inducing (Ti) plasmid into wound-activated plant cells, where it becomes integrated into nuclear DNA1 and expressed2. The vir region of the Ti plasmid is essential for T-DNA transfer3,4 and is induced by specific wound substances from plants5–9. Agrobacterium is therefore a powerful tool for gene transfer to plants, but with some exceptions10–14 this method does not seem to be applicable to the commercially important monocotyledonous crops. We report here transformation of the monocotyledonous crop plant Dioscorea bulbifera (yam) with agrobacteria preincubated by wound substances from dicotyledons, leading to crown gall tumour formation. Moreover, we present direct evidence for the integration of T-DNA into the nuclear genome of this monocotyledonous crop.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
1. Kahl, G. & Schell, J. Molecular Biology of Plant Tumors (Academic, New York, 1982). 2. Willmitzer, L. et al. Cell 32, 1045–1056 (1983). 3. Yanofsky, M. F. et al. Cell 47, 471–477 (1986). 4. Stachel, S. E. & Zambryski, P. C. Cell 47, 155–157 (1986). 5. Okker, R. J. H. et al. Nature 312, 564–566 (1984). 6. Stachel, S. E., Messens, E., van Montagu, M. & Zambryski, P. Nature 318, 624–629 (1985). 7. Stachel, S. E. & Nester, E. W. EM BO J. 5, 1445–1454 (1986). 8. Stachel, S. E., Nester, E. W. & Zambryski, P. Proc. natn. Acad. Sci. U.S.A. 83,379–383 (1986). 9. Stachel, S. E., Timmerman, B. & Zambryski, P. Nature 322, 706–712 (1986). 10. Hooykaas–van Slogteren, G. M. S., Hooykaas, P. J. J. & Schilperoort, R. A. Nature 311, 763–764(1984). 11. Hernalsteens, J. P., Thia–Toong, L., Schell, J. & van Montague, M. EMBO J. 3, 3039–3041 (1984). 12. Graves, A. C. F. & Goldman, S. L. PI. molec. Biol. 7, 43–50 (1986). 13. Grimsley, N., Hohn, B., Hohn, Th. & Walden, R. Proc. natn. Acad. Sci. U.S.A. 83,3282–3286 (1986). 14. Grimsley, N., Hohn, Th., Davies, J. W. & Hohn, B. Nature 325, 177–179 (1987). 15. DeCleene, M. & DeLey, J. Bot. Rev. 42, 389–466 (1976). 16. Rao, S. S., Lippincott, B. B. & Lippincott, J. A. Physiol. Plant. 56, 374–380 (1982). 17. Krens, F. A., Wullems, G. J. & Schilperoort, R. A. in Structure and Function of Plant Genomes (eds Ciferri, O. & Dure, L.) 387–408 (Plenum, New York, 1983). 18. Otten, L. A. B. M. & Schilperoort, R. A. Biochim. biophys. Acta 527, 497–500 (1978). 19. Christou, P., Platt, S. G. & Ackerman, M. C. PI Physiol. 82, 218–221 (1986). 20. Ammirato, P. V. in Handbook of Plant Cell Culture Vol. 3 (eds Ammirato, P. V., Evans, R., Sharp, Y. & Yamada, Y.) 327–354 (Macmillan, London, 1983). 21. Murashige, T. & Skoog, F. Physiologia Plant. 15, 473–497 (1962). 22. Depicker, A. et al Plasmid 3, 193–211 (1980). 23. Inze, D. et al. Molec. gen. Genet. 194, 265–274 (1984). 24. Willmitzer, L. & Wagner, K. Expl. Cell Res. 135, 69–77 (1981). 25. Feinberg, A. P. & Vogelstein, B. Analyt. Biochem. 132, 6–13 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schäfer, W., Görz, A. & Kahl, G. T-DNA integration and expression in a monocot crop plant after induction of Agrobacterium. Nature 327, 529–532 (1987). https://doi.org/10.1038/327529a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/327529a0
This article is cited by
-
Widespread occurrence of natural genetic transformation of plants by Agrobacterium
Plant Molecular Biology (2019)
-
Efficient shoot organogenesis in petioles of yam (Dioscorea spp)
Plant Cell, Tissue and Organ Culture (PCTOC) (2012)
-
Problems and possibilities of monocot transformation
Biologia plantarum (2011)
-
Agrobacterium-mediated transformation of Dioscorea zingiberensis Wright, an important pharmaceutical crop
Plant Cell, Tissue and Organ Culture (PCTOC) (2009)
-
Optimization of Agrobacterial (Agrobacterium tumefaciens) Transformation of Maize Embryogenic Callus
Russian Journal of Plant Physiology (2005)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
