Abstract
It is generally accepted that a globular protein in its native state adopts a single, well-defined conformation. However, there have been several reports that some proteins may exist in more than one distinct folded form in equilibrium. In the case of staphylococcal nuclease, evidence for multiple conformations has come from electrophoretic and NMR studies1–4, although there has been some controversy as to whether these are actually interconvertible forms of the same molecular species5,6. Recently, magnetization transfer(MT)-NMR has been developed as a means of studying the kinetics of conformational transitions in proteins7. In the study reported here, this approach has been extended and used to demonstrate the presence of at least two native forms of nuclease in equilibrium and to study their Interconversion with the unfolded state under the conditions of the thermal unfolding transition. The experiments reveal that two distinct native forms of the protein fold and unfold independently and that these can interconvert directly as well as via the unfolded state. The spectra of the different forms suggest that they are structurally similar but the M T experiments show that the kinetics of folding and unfolding are quite different. Characterization of this behaviour will, therefore, have important implications for our understanding of the relationship between structure and folding kinetics.
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References
Taniuchi, H. & Anfinsen, C. B. J. biol. Chem. 241, 4366–4385 (1966).
Markley, J. L., Williams, M. N. & Jardetzky, O. Proc. natn. Acad. Sci. U.S.A. 65, 645–651 (1970).
Arata, Y., Khalifah, R. & Jardetzky, O. Ann. N.Y. Acad. Sci. 222, 230–239 (1973).
Tucker, P. W., Hazen, E. E. & Cotton, F. A. Molec. cell. Biochem. 22, 67–77 (1978).
Cohen, J. S., Shrager, R. I., McNell, M. & Schechter, A. N. Nature 228, 642–644 (1970).
Marrakechi, N. & Cozzone, P. J. C. r. hebd. Séanc. Acad. Sci., Paris Ser. 3 293, 23–26 (1981).
Dobson, C. M. & Evans, P. A. Biochemistry 23, 4267–4270 (1984).
Anfinsen, C. B., Schechter, A. N. & Taniuchi, H. Cold Spring Harb. Symp. quant. Biol. 36, 243–249 (1971).
Anfinsen, C. B. Science 181, 223–230 (1973).
Tucker, P. W., Hazen, E. E. & Cotton, F. A. Molec. cell. Biochem. 23, 131–141 (1979).
Arnone, A. et al. J. biol. Chem. 246, 2302–2316 (1971).
Cotton, F. A., Hazen, E. E. & Legg, M. J. Proc. natn. Acad. Sci. U.S.A. 76, 2551–2555 (1979).
Forsén, S. & Hoffman, R. A. J. chem. Phys. 39, 2892–2901 (1963).
Campbell, I. D., Dobson, C. M., Ratcliffe, R. G. & Williams, R.J.P. J. magn. Reson. 29, 397–417 (1978).
Brandts, J. F., Halvorson, H. R. & Brennan, M. Biochemistry 14, 4953–4963 (1975).
Cook, K. H., Schmid, F. X. & Baldwin, R. L. Proc. natn. Acad. Sci. U.S.A. 76, 6157–6161 (1979).
Gronenborn, A. et al. Molec. Pharm. 20, 145–153 (1981).
Matthews, C. R., Perry, K. M. & Touchette, N. A. Am. chem. Soc. Abstr. 186, BIOL83 (1983).
Cone, J. L., Cusumano, C. L., Taniuchi, H. & Anfinsen, C. B. J. biol. Chem. 246, 3103–3110 (1971).
Fuchs, S., Cuatrecasas, P. & Anfinsen, C. B. J. biol Chem. 242, 4768–4770 (1967).
Morris, G. A. & Freeman, R. J. magn. Reson. 29, 433–462 (1978).
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Fox, R., Evans, P. & Dobson, C. Multiple conformations of a protein demonstrated by magnetization transfer NMR spectroscopy. Nature 320, 192–194 (1986). https://doi.org/10.1038/320192a0
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DOI: https://doi.org/10.1038/320192a0
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