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Simian virus 40 replication in adenovirus-transformed human cells antagonizes gene expression

Nature volume 317, pages 169–171 (1985)Cite this article

Abstract

Simian virus 40 (SV40) replicates efficiently in monkey kidney cells. However, we have now found that SV40-based vectors transfected into most human cells replicate poorly, if at all. In contrast, strong SV40 replication is observed in human embryonic kidney (HEK) cells transformed with the adenovirus early region, but not in untransformed HEK cells. Vector replication in adenovirus-transformed cells is dependent on the presence of the SV40 origin of replication and large-T antigen. However, vigorous replication occurs at levels of large-T antigen that are undetectable by immunofluorescence. These data suggest that the adenovirus oncogenes create a replication-permissive environment to which the SV40 replicon responds. Furthermore, replication and gene expression seem to be antagonistic on our vectors. High levels of large-T antigen are observed only when vector replication is blocked by mutations in the gene for large-T antigen or the origin of replication, or by direct inhibition of DNA polymerase with aphidicolin.

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Authors and Affiliations

  1. Department of Genetics, Stanford University School of Medicine, Stanford, California, 94305, USA

    Jane S. Lebkowski, Suzanne Clancy & Michele P. Calos

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  1. Jane S. Lebkowski
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  2. Suzanne Clancy
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  3. Michele P. Calos
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Lebkowski, J., Clancy, S. & Calos, M. Simian virus 40 replication in adenovirus-transformed human cells antagonizes gene expression. Nature 317, 169–171 (1985). https://doi.org/10.1038/317169a0

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