Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Enhancement of calcium current in Aplysia neurones by phorbol ester and protein kinase C

Nature volume 313, pages 313–316 (1985)Cite this article

Abstract

One of the molecular mechanisms capable of regulating the physiological properties of neurones is the phosphorylation of ion channels and other cellular components by cyclic AMP-dependent protein kinase1. Another protein kinase present in high concentrations in the mammalian brain is protein kinase C (a calcium/phosphatidylserine/diacylglycerol-dependent protein kinase)2–5, but there is no direct evidence, as yet, for the involvement of this enzyme in the control of neuronal excitability. We now present evidence that activation of endogenous protein kinase C by the tumour-promoting phorbol ester TPA (12-O-tetradecanoyl-phorbol-13-acetate), or intracellular injection of the purified enzyme, enhances the voltage-sensitive calcium current in bag cell neurones of the mollusc Aplysia.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Nestler, E. J. & Greengard, P. Nature 305, 583–588 (1983).

    Article  ADS  CAS  Google Scholar 

  2. Takai, Y., Kishimoto, A., Inoue, M. & Nishizuka, Y. J. biol. Chem. 252, 7603–7609 (1977).

    CAS  PubMed  Google Scholar 

  3. Kuo, J. F. et al. Proc. natn. Acad. Sci. U.S.A. 77, 7039–7043 (1980).

    Article  ADS  CAS  Google Scholar 

  4. Walaas, S. I., Nairn, A. C. & Greengard, P. J. Neurosci. 3, 302–311 (1983).

    Article  CAS  Google Scholar 

  5. Nishizuka, Y. Nature 308, 693–697 (1984).

    Article  ADS  CAS  Google Scholar 

  6. DeRiemer, S. A., Kaczmarek, L. K., Albert, K. A. & Greengard, P. Soc. Neurosci. Abstr. 9, 77 (1983).

    Google Scholar 

  7. DeRiemer, S. A. thesis, Yale Univ. (1984).

  8. Kaczmarek, L. K. & Strumwasser, F. J. Neurophysiol. 52, 340–349 (1984).

    Article  CAS  Google Scholar 

  9. Tsien, R. Y., Pozzan, T. & Rink, T. J. Nature 295, 68–70 (1982).

    Article  ADS  CAS  Google Scholar 

  10. Strong, J. A. J. Neurosci. 4, 2772–2783 (1984).

    Article  CAS  Google Scholar 

  11. Strong, J. A. & Kaczmarek, L. K. Neurosci. Abstr. 10, 1101 (1984).

    Google Scholar 

  12. Kaczmarek, L. K., Jennings, K. & Strumwasser, F. Proc. natn. Acad. Sci. U.S.A. 77, 5200–5204 (1978).

    Article  ADS  Google Scholar 

  13. Marban, E. & Tsien, R. W. J. Physiol., Lond. 329, 589–614 (1982).

    Article  CAS  Google Scholar 

  14. Kaczmarek, L. K. & Strumwasser, F. J. Neurosci. 1, 626–634 (1981).

    Article  CAS  Google Scholar 

  15. Kikkawa, U., Takai, Y., Minakuchi, R., Inohara, S. & Nishizuka, Y. J. biol. Chem. 257, 13341–13348 (1982).

    CAS  Google Scholar 

  16. Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Pflügers. Arch. ges. Physiol. 391, 85–100 (1981).

    Article  CAS  Google Scholar 

  17. Kostyuk, P. G. Physiol. Rev. 64, 435–454 (1984).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. S. A. DeRiemer

    Present address: Max-Planck-Institut für biophysikalische Chemie, Postfach 968, D-3400, Göttingen, FRG

  2. K. A. Albert and P. Greengard: The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA

Authors and Affiliations

  1. Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut, 06510, USA

    S. A. DeRiemer, J. A. Strong, K. A. Albert, P. Greengard & L. K. Kaczmarek

Authors
  1. S. A. DeRiemer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. A. Strong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. A. Albert
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Greengard
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. K. Kaczmarek
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

DeRiemer, S., Strong, J., Albert, K. et al. Enhancement of calcium current in Aplysia neurones by phorbol ester and protein kinase C. Nature 313, 313–316 (1985). https://doi.org/10.1038/313313a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/313313a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing