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Molecular cloning of a cDNA encoding human antihaemophilic factor

Nature volume 312, pages 342–347 (1984)Cite this article

Abstract

A complete copy of the mRNA sequences encoding human coagulation factor VIII:C has been cloned and expressed. The DNA sequence predicts a single chain precursor of 2,351 amino acids with a relative molecular mass (Mr) 267,039. The protein has an obvious domain structure, contains sequence repeats and is structurally related to factor V and ceruloplasmin.

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References

  1. Hoyer, L. W. Blood 58, 1–12 (1981).

    CAS  Google Scholar 

  2. Rapaport, S. I., Schiffman, S. & Patch, M. J. Blood 21, 221–235 (1963).

    CAS  PubMed  Google Scholar 

  3. Vehar, G. A. & Davie, E. W. Biochemistry 19, 410–416 (1980).

    Article  Google Scholar 

  4. Fass, D. N., Knutson, G. J. & Katzman, J. A. Blood 59, 594–600 (1982).

    CAS  PubMed  Google Scholar 

  5. Fulcher, C. A., Gardiner, J. E., Griffin, J. H. & Zimmerman, T. A. Blood 63, 486–489 (1984).

    CAS  PubMed  Google Scholar 

  6. Fass, D. N., Hewick, R. M., Knutson, G. J., Nesheim, M. E. & Mann, K. G. Proc. natn. Acad. Sci. U.S.A. (in the press).

  7. Owen, C. A., Jr Bowie, W. E. J. & Fass, D. N. Br. J. Haemat. 43, 307–315 (1979).

    Article  CAS  Google Scholar 

  8. Libre, E. P., Cowen, D. H., Watkins, S. P. & Shulman, N. R. Blood 31, 358–368 (1968).

    CAS  PubMed  Google Scholar 

  9. Marchioro, T. L., Houghie, C., Ragde, H., Epstein, R. B. & Thomas, E. D. Science 163, 188–190 (1969).

    Article  ADS  CAS  Google Scholar 

  10. Knutson, G. J. & Fass, D. N. Blood 59, 615–624 (1982).

    CAS  PubMed  Google Scholar 

  11. Hewick, R. M., Hunkapiller, M. E., Hood, L. E. & Dreyer, W. J. J. biol. Chem. 256, 7990–7997 (1982).

    Google Scholar 

  12. Strehler, B. & North, D. Mech. Ageing Dev. 18, 285–313 (1982).

    Article  CAS  Google Scholar 

  13. Agarwal, K. L., Brunstedt, J. & Noyes, B. E. J. biol. Chem. 256, 1023–1028 (1981).

    CAS  PubMed  Google Scholar 

  14. Jaye, M. et al. Nucleic Acids Res. 11, 2325–2335 (1983).

    Article  CAS  Google Scholar 

  15. Lawn, R. M., Fritsch, E. F., Parker, R. C., Blake, G. & Maniatis, T. Cell 15, 1157–1174 (1978).

    Article  CAS  Google Scholar 

  16. Stel, H. V., vanderKwast, Th. H. & Veerman, E. C. I. Nature 303, 530–532 (1983).

    Article  ADS  CAS  Google Scholar 

  17. Kaufman, R. J. & Sharp, P. A. Molec. Cell Biol. 2, 1304–1319 (1982).

    Article  CAS  Google Scholar 

  18. Clark, S. C. et al. Proc. natn. Acad. Sci. U.S.A. 81, 2541–2547 (1984).

    ADS  Google Scholar 

  19. Hopp, T. P. & Woods, K. R. Proc. natn. Acad. Sci. U.S.A. 78, 3824–3828 (1981).

    Article  ADS  CAS  Google Scholar 

  20. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).

    Article  CAS  Google Scholar 

  21. Church, W. R. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).

  22. Takahashi, N., Ortel, T. L. & Putnam, F. W. Proc. natn. Acad. Sci. U.S.A. 81, 390–394 (1984).

    Article  ADS  CAS  Google Scholar 

  23. Gluzman, Y. Cell 23, 175–182 (1981).

    Article  CAS  Google Scholar 

  24. Sompayrac, L. M. & Dana, K. J. Proc. natn. Acad. Sci. U.S.A. 78, 7575–7578 (1981).

    Article  ADS  CAS  Google Scholar 

  25. Luthman, H. & Magnusson, G. Nucleic Acids Res. 11, 1295–1308 (1983).

    Article  CAS  Google Scholar 

  26. Lee, M. L., Maglalang, E. A. & Kingdon, H. S. Thromb. Res. 30, 511–519 (1983).

    Article  CAS  Google Scholar 

  27. Kasper, C. K. Thromb. Diath. Haemorrh 34, 869–872 (1975).

    Google Scholar 

  28. Hunkapillar, M. W., Lujan, E., Ostrander, F. & Hood, L. E. Meth. Enzym. 91, 227–236 (1983).

    Article  Google Scholar 

  29. Woo, S. L. C. Meth. Enzym. 68, 389–395 (1979).

    Article  CAS  Google Scholar 

  30. Hastie, N. D., Held, W. & Toole, J. J. Cell 17, 449–457 (1979).

    Article  CAS  Google Scholar 

  31. Derman, E. et al. Cell 23, 731–739 (1981).

    Article  CAS  Google Scholar 

  32. O'Hare, K., Levis, R. & Rubin, G. M. Proc. natn. Acad. Sci. U.S.A. 80, 6917–6921 (1983).

    Article  ADS  CAS  Google Scholar 

  33. Shaw, P. H., Held, W. A. & Hastie, N. D. Cell 32, 755–761 (1983).

    Article  CAS  Google Scholar 

  34. Gubler, U. & Hoffman, B. J. Gene 25, 163–269 (1983).

    Article  Google Scholar 

Download references

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Author notes

  1. Gaylord J. Knutson and David N. Fass: Hematology Research Section, Mayo Clinic/Foundation, Rochester, Minnesota 55905, USA

Authors and Affiliations

  1. Genetics Institute, Inc., 225 Longwood Avenue, Boston, Massachusetts, 02115, USA

    John J. Toole, John L. Knopf, John M. Wozney, Lisa A. Sultzman, Janet L. Buecker, Debra D. Pittman, Randal J. Kaufman, Eugene Brown, Charles Shoemaker, Elizabeth C. Orr, Godfrey W. Amphlett, W. Barry Foster, Mary Lou Coe, Gaylord J. Knutson, David N. Fass & Rodney M. Hewick

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  1. John J. Toole
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  2. John L. Knopf
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  6. Debra D. Pittman
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  7. Randal J. Kaufman
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  14. Gaylord J. Knutson
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  15. David N. Fass
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  16. Rodney M. Hewick
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Toole, J., Knopf, J., Wozney, J. et al. Molecular cloning of a cDNA encoding human antihaemophilic factor. Nature 312, 342–347 (1984). https://doi.org/10.1038/312342a0

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  • DOI: https://doi.org/10.1038/312342a0

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