Abstract
Ionic fluxes are thought to he involved in mediating the prolifer-ation of peripheral blood lymphocytes (PBLs) in response to mitogenic substances1,2. Among the earliest events occurring after the addition of mitogen to cultured lympocytes are changes in rates of cation transport3–9. We were interested, therefore, in the possible role of ion channels in mediating the lymphocyte proliferative response. The development of patch clamp tech-niques by Neher and colleagues10–12 has made it possible to study membrane conductances in a variety of small cell types12. We have developed a method which uses monoclonal antibodies to make cells adhere to solid surfaces for two major reasons: (1) it is much easier to patch clamp stationary cells, and (2) the method can be used to selectively adhere a particular cell type from a heterogeneous population. We have used these techniques here to identify whole-cell potassium currents, in lymphocytes recognized by OKT11 monoclonal antibody, which are increased 1.9-fold by mitogenic stimulation.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Leffert, H. Ann. N.Y. Acad. Sci. 339, 74–262 (1980).
Cameron, I. L. & Pool, T. B. (eds) The Transformed Cell, 1–420 (Academic, New York, 1981).
Kaplan, J. G. A. Rev. Physiol. 40, 19–41 (1978).
Kaplan, J. G. & Owen, T. Ann. N.Y. Acad. Sci. 339, 191–200 (1980).
Allwood, G., Asherton, G. L., Davey, M. J. & Goodford, P. J. Immunology 21, 509–516 (1971).
Deutsch, C. & Price, M. Biochim. biophys. Acta 687, 211–218 (1982).
Whitney, R. B. & Sutherland, R. M. Cell. Immun. 5, 137–147 (1972).
Parker, C. W. Biochem. biophys. Res. Commun. 61, 1180–1186 (1974).
Freedman, N. H., Raff, M. C. & Gomperts, B. Nature 255, 378–382 (1975).
Neher, E. & Sakmann, B. Nature 260, 799–802 (1976).
Neher, E., Sakmann, B. & Steinbach, J. H. Pflugers Arch. ges. Physiol 375, 219–228 (1978).
Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Pflugers Arch. ges. Physiol. 391, 85–100 (1981).
Reinherz, E. L. & Schlossman, S. F. Immun. Today 2, 69–75 (1981).
Haynes, B. F. Immun. Rev. 57, 127–161 (1981).
Grinstein, S., Dupre, A. & Rothstein, A. J. gen. Physiol. 79, 849–868 (1982).
Bezanilla, F. & Armstrong, C. M. J. gen. Physiol. 60, 588–608 (1972).
French, R. J. & Adelman, W. J. Jr Curr. Topics Membranes Transport 8, 161–207 (1976).
Segel, G. B., Hollander, M. M., Gordon, B. R., Klemperer, M. R. & Lichtman, M. A. J. cell. Physiol. 86, 327–336 (1975).
Hamilton, L. J. & Kaplan, J. G. Can. J. Biochem. 55, 774–778 (1977).
Segel, G. B., Simon, W. & Lichtman, M. A. J. clin. Invest. 64, 834–841 (1979).
Tsien, R. Y., Pozzan, T. & Rink, T. J. Nature 295, 68–70 (1982).
Hagiwara, S. & Byerly, L. A. Rev. Neurosci. 4, 69–125 (1981).
Fukushima, Y. & Hagiwara, S. Biophys. J. 41, 292a (1983).
Moyer, M. P., Moyer, R. C. & Waite, M. R. F. in Ions, Cell Proliferation and Cancer Symp., 53 (National Cancer Institute and W. Alton Jones Cell Science Center, Lake Placid, New York, 1982).
Deutsch, C., Price, M. & Johansson, C. Expl Cell Res. 136, 359–369 (1981).
Armstrong, C. M. & Bezanilla, F. J. gen. Physiol. 63, 533–552 (1974).
Matteson, D. R. & Armstrong, C. M. J. gen. Physiol. (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Matteson, D., Deutsch, C. K channels in T lymphocytes: a patch clamp study using monoclonal antibody adhesion. Nature 307, 468–471 (1984). https://doi.org/10.1038/307468a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/307468a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
