Abstract
Actin is found in almost all kinds of non-muscle cells where it is thought to have an important role in cell motility. A proper understanding of that role will only be possible when reliable in vitro systems are available for investigating the interaction of cellular actin and myosin. A start has been made on several systems1–4, most recently by Sheetz and Spudich who demonstrated unidirectional movement of HMM-coated beads along F-actin cables on arrays of chloroplasts exposed by dissection of a Nitella cell5. As an alternative approach, we report here the direct observation by fluorescence microscopy of the movements of single F-actin filaments interacting with soluble myosin fragments energized by Mg2+-ATP.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Oplatka, A. & Tirosh, R. Biochim. biophys. Acta 305, 683–688 (1973).
Yano, M., Yamamoto & Shimizu, H. Nature 299, 557–559 (1982).
Kuroda, K. & Kamiya, N. Proc. Japan. Acad. 51, 774–777 (1975).
Higashi-Fujime, S. J. Cell Biol. 87, 569–578 (1980).
Sheetz, M. P. & Spudich, J. A. Nature 303, 31–35 (1983).
Nagashima, H. & Asakura, S. J. molec. Biol. 136, 169–182 (1980).
Wulf, E., Deboben, A., Bautz, F. A., Faulstich, H. & Wieland, Th. Proc. natn. Acad. Sci. U.S.A. 76, 4498–4502 (1979).
Pròchniewicz-Nakayama, E., Yanagida, T. & Oosawa, F. J. Cell Biol. (in the press).
Fujime, S. J. Phys. Soc. Japan 29, 751–759 (1970).
Yanagida, T. & Oosawa, F. J. molec. Biol. 126, 509–524 (1978).
Yoshino, S., Umazume, Y., Natori, R., Fujime, S. & Chiba, S. Biophys. Chem. 8, 317–326 (1978).
Wieland, Th. & Faulstich, H. CRC crit. Rev. Biochem. 5, 185–260 (1978).
Eisenberg, E. & Moos, C. J. biol. Chem. 242, 2945–2951 (1967).
Knight, P. & Offer, G. Biochemistry 19, 4682–4687 (1980).
Yanagida, T. in Cross-bridge Mechanism in Muscle Contraction (eds Pollack, G. H. & Sugi, H.) (Plenum, New York, in the press).
Landau, L. D. & Lifshitz, E. M. Statistical Physics, 478–482 (Pergamon, London, (1958).
Harris, R. A. & Hearst, J. E. J. chem. Phys. 44, 2595–2609 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yanagida, T., Nakase, M., Nishiyama, K. et al. Direct observation of motion of single F-actin filaments in the presence of myosin. Nature 307, 58–60 (1984). https://doi.org/10.1038/307058a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/307058a0
This article is cited by
-
Single molecule turnover of fluorescent ATP by myosin and actomyosin unveil elusive enzymatic mechanisms
Communications Biology (2021)
-
Photoblueing of organic dyes can cause artifacts in super-resolution microscopy
Nature Methods (2021)
-
A myosin II nanomachine mimicking the striated muscle
Nature Communications (2018)
-
Polymerization and depolymerization of actin with nucleotide states at filament ends
Biophysical Reviews (2018)
-
The emergence and transient behaviour of collective motion in active filament systems
Nature Communications (2017)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
