Abstract
Using previously isolated mouse L-cell transferees for the human T-cell differentiation antigen Leu-2, we now report the first example of spontaneous gene amplification for membrane antigens. The Leu-2 (or T8)1,2 antigen is normally expressed on T lymphocytes that have cytotoxic or suppressor functions. Cells of a Leu-2 transfected clone were stained with fluorescein-tagged monoclonal anti-Leu-2, and the brightest 0.1–0.3% of cells were viably separated using a fluorescence activated cell sorter (FACS). After growth of these selected cells, sorting and regrowth was repeated six times, resulting in a population of cells that, compared with the starting population, stains 40 times brighter for Leu-2 and whose DNA transforms 20 times more efficiently for Leu-2. In addition, these cells have 10- to 50-fold amplified human DNA sequences and numerous double minute chromosome fragments, a common indicator of gene amplication in mouse cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ledbetter, J. A. et al. J. exp. Med. 153, 310–323 (1981).
Snow, P., Spits, H., deVries, J. & Terhorst, C. Hybridoma (in the press).
Kavathas, P. & Herzenberg, L. A. Proc. natn. Acad. Sci. U.S.A. 80, 524–528 (1983).
Kaufman, R. J., Brown, P. C. & Schimke, R. T. Proc. natn. Acad. Sci. U.S.A. 76, 5669–5673 (1979).
Brown, P. C., Beverley, S. M. & Schimke, R. T. Molec. cell. Biol. 1, 1077–1083 (1983).
Perucho, M., Hanahan, D. & Wigler, M. Cell 22, 309–317 (1980).
Johnston, R. N., Beverley, S. M. & Schimke, R. T. Proc. natn. Acad. Sci. U.S.A. (in the press).
Brennand, J., Chinault, A. C., Konecki, D. S., Melton, D. W. & Caskey, C. T. Proc. natn. Acad. Sci. U.S.A. 79, 1950–1954 (1982).
George, D. L. & Powers, V. E. Cell 24, 117–123 (1981).
Shih, C. & Weinberg, R. A. Cell 29, 161–169 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kavathas, P., Herzenberg, L. Amplification of a gene coding for human T-cell differentiation antigen. Nature 306, 385–387 (1983). https://doi.org/10.1038/306385a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/306385a0
This article is cited by
-
Calcium-dependent phospholipid scrambling by TMEM16F
Nature (2010)
-
Isolation of the CD7 gene from the DNA of transfected L cells
Immunogenetics (1987)
-
Gene transfection of the HuLy-m2 (Leu-9) antigen into mouse L cells
Immunogenetics (1987)
-
Isolation of complementary DNA clones encoding the human lymphocyte glycoprotein T1/Leu-1
Nature (1986)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.