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Functional expression of a transfected murine class II MHC gene

Nature volume 306, pages 190–194 (1983)Cite this article

Abstract

The activation of T helper lymphocytes involves the recognition of class U major histocompatibility complex antigens, which are dimeric glycoproteins (of subunit composition AαAβ or EαEβ) expressed on the surfaces of macrophages and B lymphocytes1–5. One approach to understanding the relationship between the structure of these antigens and their functions in the immune response6,7 is to clone the genes that encode them8–13, to obtain functional expression of the cloned genes transfected into an appropriate cell line, and then to see how those functions are affected in variant genes generated in vitro. We report here the expression in Iad-bearing B cells of an Akβ gene, which confers on the transfected cells the capacity for both allostimulation and antigen-dependent activation of an I–Ak-restricted T-cell clone.

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Author notes

  1. David H. Margulies

    Present address: Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 2020S, USA

Authors and Affiliations

  1. Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20205, USA

    Ronald N. Germain & Michael A. Norcross

  2. Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20205, USA

    David H. Margulies

Authors
  1. Ronald N. Germain
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  2. Michael A. Norcross
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  3. David H. Margulies
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Germain, R., Norcross, M. & Margulies, D. Functional expression of a transfected murine class II MHC gene. Nature 306, 190–194 (1983). https://doi.org/10.1038/306190a0

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