Abstract
Mouse L cells transformed with the cloned class I genes of the major histocompatibility complex of the mouse express transplantation antigens with serological determinants of the donor haplotype. However, transformation with the truncated subclones of a BALB/c H-2Ld gene containing the exons encoding the external domains also leads to the production of cells which express complete cell-surface molecules. Moreover, full-length products of the foreign haplotype, as judged by serological and biochemical criteria, are generated independently of the use of carrier DNA in transformation. However, the frequency of productive transformation is substantially less than that obtained with a complete gene. The most plausible explanation for these phenomena involves homologous recombination between host chromosomal and donor class I sequences.
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References
Gorer, P. A. J. Path. Bact. 47, 231–252 (1938).
Gorer, P. A., Lyman, S. & Snell, G. D. Proc. R. Soc. B135, 499–505 (1948).
Zinkernagel, R. M. & Doherty, P. C. Adv. Immun. 27, 51–177 (1980).
Shearer, G. M. & Schmitt-Verhulst, A. M. Adv. Immun. 25, 55–91 (1977).
Vitetta, E. S., Poulik, N. D., Klein, J. & Uhr, J. W. J. exp. Med. 144, 179–192 (1976).
Krange, M. S., Orr, H. T. & Strominger, J. L. Cell 18, 979–991 (1979).
Lopez de Castro, J. A. et al. Biochemistry 18, 5704–5711 (1979).
Coligan, J. E., Kindt, T. J., Uehara, H., Martinko, J. & Nathenson, S. G. Nature 291, 35–39 (1981).
Steinmetz, M., Winoto, A., Minard, K. & Hood, L. Cell 28, 489–498 (1982).
Steinmetz, M. et al. Cell 24, 125–134 (1981).
Steinmetz, M. S. et al. Cell 25, 683–692 (1981).
Goodenow, R. S. et al. Science 215, 677–679 (1982).
Goodenow, R. S. et al. Nature 300, 231–237 (1982).
Moore, K. W., Sher, B. T., Sun, H. Y., Eakle, K. A. & Hood, L. Science 215, 697–682 (1982).
Evans, G. A., Margulies, D. H., Camerini-Otero, R. E., Ozato, K. & Seidmann, J. G. Proc. natn. Acad. Sci. U.S.A. 79, 1994–1998 (1982).
Mellor, A. L. et al. Nature 298, 529–533 (1982).
Martinko, J. M. et al. Biochemistry 19, 6188–6193 (1980).
Uehara, H., Coligan, J. E. & Nathenson, S. G. Biochemistry 20, 5940–5945 (1981).
Pellicer, A. et al. Science 209, 1414–1422 (1980).
Perucho, M., Hanahan, D. & Wigler, M. Cell 22, 309–317 (1980).
Robins, D. M., Ripley, S., Henderson, A. S. & Axel, R. Cell 23, 29–39 (1981).
Anderson, R. A., Krakauer, T. & Camerini-Otero, D. R. Proc. natn. Acad. Sci. U.S.A. 79, 2748–2752 (1982).
Roberts, J. M. & Axel, R. Cell 29, 109–119 (1982).
Radding, C. M. A. Rev. Biochem. 47, 847–880 (1978).
Stahl, F. W. Genetic Recombination (Freeman, San Francisco, 1979).
Winocour, E. & Keshet, I. Proc. natn. Acad. Sci. U.S.A. 77, 4861–4865 (1980).
Varmus, H. E., Quintrell, N. & Ortiz, S. Cell 25, 23–36 (1981).
Radding, C. M. Cell 25, 3–4 (1981).
Hinnen, A. H., Hicks, J. B. & Fink, G. R. Proc. natn. Acad. Sci. U.S.A. 75, 1929–1933 (1978).
Szybalska, E. H. & Szybalski, W. Proc. natn. Acad. Sci. U.S.A. 48, 2026–2034 (1962).
Ozato, K., Hansen, T. H. & Sachs, D. H. J. Immun. 125, 2473–2477 (1980).
Chang, A. C. Y. & Cohen, S. J. J. Bact. 134, 1141–1156 (1978).
Bolivar, F. et al. Gene 2, 95–133 (1977).
Stroynowski, I., van Cleemput, M. & Yanofsky, C. Nature 298, 38–41 (1982).
Ozato, K. & Sachs, D. H. J. Immun. 126, 317–321 (1981).
Oi, V. T., Jones, P. P., Goding, J. W., Herzenberg, L. A. & Herzenberg, L. A. Curr. Topics Microbiol. Immun. 81, 115–129 (1978).
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Goodenow, R., Stroynowski, I., McMillan, M. et al. Expression of complete transplantation antigens by mammalian cells transformed with truncated class I genes. Nature 301, 388–394 (1983). https://doi.org/10.1038/301388a0
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DOI: https://doi.org/10.1038/301388a0
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