Abstract
An extragenic suppressor mutation, mim2–1, which compensates yeast mitochondrial mutants deficient in splicing of the cytochrome b gene, has been mapped and sequenced. The mutation is due to a single G → A transition in the long open reading frame of the fourth intron of the oxidase subunit one gene. It causes the replacement of a glutamic codon by a lysine codon and the expression of a novel mRNA maturase active in splicing. Evolution and regulatory connections between homologous introns of nonhomologous genes are discussed.
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Dujardin, G., Jacq, C. & Slonimski, P. Single base substitution in an intron of oxidase gene compensates splicing defects of the cytochrome b gene. Nature 298, 628–632 (1982). https://doi.org/10.1038/298628a0
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DOI: https://doi.org/10.1038/298628a0
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