Abstract
Cell-surface markers are useful in identifying and studying different cell types in the nervous system. For example, the major cell types in rat dorsal root ganglion (DRG) cultures have been identified using a combination of tetanus toxin and antibodies to the cell-surface antigens Ran-1 and Thy-1. Neurones bind tetanus toxin and express Thy-1, Schwann cells express only Ran-1 and fibroblasts express only Thy-1 (ref. 1). A natural extension of this approach was to use antibodies to distinguish between different neuronal subpopulations on the basis of their cell-surface antigens. We therefore immunized mice with cells from rat DRG cultures, and then used the hybridoma technique of Köhler and Milstein2 to produce monoclonal antibodies. We report here an antibody that recognizes a surface antigen which is present on all rat peripheral neurones we have studied and absent from neurones derived from the central nervous system. An antigen with reciprocal distribution, expressed only by neurones of the rat central nervous system, has been defined by Cohen and Selvendran3.
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Vulliamy, T., Rattray, S. & Mirsky, R. Cell-surface antigen distinguishes sensory and autonomic peripheral neurones from central neurones. Nature 291, 418–420 (1981). https://doi.org/10.1038/291418a0
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DOI: https://doi.org/10.1038/291418a0
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