Abstract
Light stimulates the uptake of polypeptides synthesized in vitro into intact chloroplasts. The light-stimulated uptake is inhibited by uncouplers, but not by the electron transport inhibitor dichlorophenyldimethylurea (DCMU) or the protein synthesis inhibitor chloramphenicol(CAP). Addition of ATP to the uptake mixture in the dark mimics the light stimulation of transport, and it reverses the uncoupler inhibition of transport in the light. These data demonstrate that cytoplasmically synthesized polypeptides are imported into the chloroplast by an energy-dependent process.
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Grossman, A., Bartlett, S. & Chua, NH. Energy-dependent uptake of cytoplasmically synthesized polypeptides by chloroplasts. Nature 285, 625–628 (1980). https://doi.org/10.1038/285625a0
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DOI: https://doi.org/10.1038/285625a0
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